PrsA - Revision history

Jstuelk: /* Original publications */

2014-12-23T17:30:38Z

‎Original publications

Jstuelk: /* References */

2014-09-19T10:37:50Z

‎References

134.76.70.252 at 09:43, 17 April 2014

2014-04-17T09:43:30Z

134.76.70.252 at 11:44, 8 April 2014

2014-04-08T11:44:45Z

134.76.38.147 at 13:19, 2 April 2014

2014-04-02T13:19:37Z

Jstuelk at 12:30, 31 January 2014

2014-01-31T12:30:53Z

Jstuelk: /* Expression and regulation */

2014-01-31T12:28:15Z

‎Expression and regulation

Jstuelk at 16:28, 16 January 2014

2014-01-16T16:28:00Z

134.76.70.252 at 15:55, 11 November 2013

2013-11-11T15:55:07Z

134.76.70.252 at 12:53, 16 May 2013

2013-05-16T12:53:47Z

← Older revision		Revision as of 17:30, 23 December 2014
Line 1:		Line 1:
−	* '''Description:''' lipoprotein, post-translocational folding of exported proteins (post-translocation molecular chaperone) <br/><br/>	+	{{#bank: PrsA}}
−	{~~\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''prsA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| yes~~ {~~{PubMed\|20487272,12682299}}~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| post-translocation molecular chaperone~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| protein folding~~
−	\|-
−	~~\|colspan="2" style="background:~~#~~FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]'''~~: ~~[http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU09950 prsA]~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PrsA PrsA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=PrsA PrsA]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 32 kDa, 9.122~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 876 bp, 292 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[yhaL]]'', ''[[sscA]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU09950 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU09950 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU09950 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:prsA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	~~\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=prsA_1070364_1071242_-1 Expression at a glance]'''   {{PubMed\|22383849}~~}~~<br/>[[Image:prsA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU09950]]~~
−	\|-
−	\|}

−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[protein secretion]]}},~~
−	~~{{SubtiWiki category\|[[essential genes]]}},~~
−	~~{{SubtiWiki category\|[[membrane proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU09950
−
−	~~===Phenotypes of a mutant ===~~
−	* essential {{PubMed\|20487272,12682299}}
−	* the mutant is viable in the presence of elevated concentrations of Mg<sup>2+</sup> {{PubMed\|20487272}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU09950&redirect=T BSU09950]
−
−	* '''DBTBS entry:''' no entry
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10464]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−	** catalyses the post-translocational folding of exported proteins {{PubMed\|20487272}}, required for folding of [[penicillin-binding proteins]] ([[PbpA]], [[PbpB]], [[PbpC]], [[PbpD]]) {{PubMed\|20487272}}
−
−	* '''Protein family:''' PpiC domain (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−	** PrsA is a lipoprotein with a N-acetyl-S-diacyl-glyceryl-cysteine structure {{PubMed\|22303020}}
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** dimeric or oligomeric protein {{PubMed\|20487272}}
−
−	* '''[[Localization]]:'''
−	** membrane associated (lipoprotein) {{PubMed\|22303020}}
−	** distinct spots organized in a helical pattern along the cell membrane {{PubMed\|20487272}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU09950&redirect=T BSU09950]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1ZK6 1ZK6]
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P24327 P24327]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU09950]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/5.2.1.8 5.2.1.8]
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[prsA]]'' {{PubMed\|22383849}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=prsA_1070364_1071242_-1 prsA] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** [[PrsA]] is subject to degradation by [[WprA]] and other extracellular proteases {{PubMed\|24362423}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 1845 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 5530 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~== Reviews ==~~
−	~~<pubmed> 25212246 </pubmed>~~
−	~~== Original publications ==~~
−	~~<pubmed>12634326,14976191,11807061,10096076,18763711, 10871614 20487272 22303020 24362423 </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 147: / Line 147: @@
 =References=
+== Reviews ==
 <pubmed>12634326,14976191,11807061,10096076,18763711, 10871614 20487272 22303020 24362423 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 125: / Line 125: @@
 * '''Additional information:'''
 ** [[PrsA]] is subject to degradation by [[WprA]] and other extracellular proteases {{PubMed|24362423}}
 =Biological materials =

@@ Line 17: / Line 17: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PrsA PrsA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/wiki/index.php/Protein_secretion Protein secretion]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in  [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=PrsA PrsA]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 9.122

@@ Line 59: / Line 59: @@
 === Database entries ===
 * '''DBTBS entry:''' no entry
@@ Line 98: / Line 99: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1ZK6 1ZK6]

@@ Line 7: / Line 7: @@
 |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 |-
-|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
+|style="background:#ABCDEF;" align="center"| '''Essential''' || yes {{PubMed|20487272,12682299}}
 |-
 |style="background:#ABCDEF;" align="center"| '''Product''' || post-translocation molecular chaperone
@@ Line 55: / Line 55: @@
 ===Phenotypes of a mutant ===
+* essential {{PubMed|20487272,12682299}}
-essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
+* the mutant is viable in the presence of elevated concentrations of Mg<sup>2+</sup> {{PubMed|20487272}}
 === Database entries ===

@@ Line 111: / Line 111: @@
 =Expression and regulation=
-* '''Operon:'''
+* '''Operon:''' ''[[prsA]]'' {{PubMed|22383849}}
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=prsA_1070364_1071242_-1 prsA] {{PubMed|22383849}}

@@ Line 1: / Line 1: @@
-* '''Description:''' post-translocational folding of exported proteins (post-translocation molecular chaperone) <br/><br/>
+* '''Description:''' lipoprotein, post-translocational folding of exported proteins (post-translocation molecular chaperone) <br/><br/>
 {| align="right" border="1" cellpadding="2"
 |-
@@ Line 38: / Line 38: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/>
@@ Line 74: / Line 70: @@
 === Basic information/ Evolution ===
-* '''Catalyzed reaction/ biological activity:''' catalyses the post-translocational folding of exported proteins {{PubMed|20487272}}, required for folding of [[penicillin-binding proteins]] ([[PbpA]], [[PbpB]], [[PbpC]], [[PbpD]]) {{PubMed|20487272}}
+* '''Catalyzed reaction/ biological activity:'''
 * '''Protein family:''' PpiC domain (according to Swiss-Prot)
@@ Line 84: / Line 81: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
 ** PrsA is a lipoprotein with a N-acetyl-S-diacyl-glyceryl-cysteine structure {{PubMed|22303020}}
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 118: / Line 115: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=prsA_1070364_1071242_-1 prsA] {{PubMed|22383849}}
-* '''Sigma factor:'''
+* '''[[Sigma factor]]:'''
 * '''Regulation:'''
@@ Line 125: / Line 122: @@
 * '''Additional information:'''
 =Biological materials =
@@ Line 146: / Line 144: @@
 =References=
-<pubmed>12634326,14976191,11807061,10096076,18763711, 10871614 20487272 22303020</pubmed>
+<pubmed>12634326,14976191,11807061,10096076,18763711, 10871614 20487272 22303020 24362423 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 15: / Line 15: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU09950 prsA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PrsA PrsA]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PrsA PrsA]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/wiki/index.php/Protein_secretion Protein secretion]'''

@@ Line 30: / Line 30: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=prsA_1070364_1071242_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:prsA_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=prsA_1070364_1071242_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:prsA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU09950]]
 |-
 |}