PlsX - Revision history

134.76.70.252 at 14:11, 17 April 2014

2014-04-17T14:11:56Z

134.76.70.252 at 09:44, 17 April 2014

2014-04-17T09:44:36Z

134.76.38.147 at 13:42, 2 April 2014

2014-04-02T13:42:12Z

Jstuelk at 19:21, 12 January 2014

2014-01-12T19:21:23Z

Jstuelk at 19:18, 12 January 2014

2014-01-12T19:18:53Z

134.76.70.252 at 10:46, 7 January 2014

2014-01-07T10:46:03Z

134.76.70.252 at 16:15, 11 November 2013

2013-11-11T16:15:55Z

134.76.70.252 at 13:20, 16 May 2013

2013-05-16T13:20:01Z

134.76.70.252 at 10:12, 14 May 2013

2013-05-14T10:12:26Z

134.76.70.252 at 12:48, 13 May 2013

2013-05-13T12:48:46Z

← Older revision		Revision as of 14:11, 17 April 2014
Line 1:		Line 1:
−	* '''Description:''' acyl-acyl carrier protein (ACP):phosphate acyltransferase, catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4), coordinates membrane synthesis with [[cell division]] <br/><br/>	+	{{#bank: PlsX}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''plsX''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''ylpD ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| yes {{PubMed\|24224907,12682299}}~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| acyl-acyl carrier protein (ACP):phosphate acyltransferase~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| biosynthesis of phospholipids~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15890 plsX]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PlsX PlsX]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=plsX plsX]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 35 kDa, 5.492~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 999 bp, 333 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[fapR]]'', ''[[fabD]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15890 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15890 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15890 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:plsX_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=plsX_1662547_1663548_1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:plsX_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15890]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[cell division]]}},~~
−	~~{{SubtiWiki category\|[[biosynthesis of lipids]]}},~~
−	~~{{SubtiWiki category\|[[essential genes]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[ComA regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[FapR regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU15890
−
−	~~===Phenotypes of a mutant ===~~
−	* essential {{PubMed\|24224907,12682299}}
−	* depletion results in sporulation deficiency (via [[SpoIIR]]) {{PubMed\|22111992}}
−	* a ts mutant forms an abberant Z-ring and has a [[cell division]] defect {{PubMed\|24224907}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15890&redirect=T BSU15890]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fapR-plsX-fabDG.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11843]
−
−	~~=== Additional information===~~
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4)
−
−	* '''Protein family:''' plsX family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[PlsX]]-[[PlsY]] {{PubMed\|19282621}}
−	** [[PlsX]]-[[FtsA]] {{PubMed\|24224907}}
−
−	* '''[[Localization]]:'''
−	** localizes to the cell division sites, colocalizes with the Z-ring {{PubMed\|24224907}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15890&redirect=T BSU15890]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1VI1 1VI1]
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P71018 P71018]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15890]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[fapR]]-[[plsX]]-[[fabD]]-[[fabG]]-[[acpA]]'' {{PubMed\|12737802,19850612}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=plsX_1662547_1663548_1 plsX] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigA]] {{PubMed\|16932747}}
−
−	* '''Regulation:'''
−	** induced if the cells experience an accumulation of malonyl-CoA ([[FapR]]) {{PubMed\|12737802}}
−	** expressed at high cell density ([[ComA]]) {{PubMed\|16091051}}
−	** induced upon fatty acid biosynthesis inhibition {{PubMed\|21383089}}
−
−	* '''Regulatory mechanism:'''
−	** [[ComA]]: transcription activation ([[ComA]]) {{PubMed\|16091051}}
−	** [[FapR]]: transcription repression {{PubMed\|12737802}}
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 237 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 743 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in Matsumoto lab {{PubMed\|19282621}}
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~<pubmed>12737802, 19282621, 17645809, 17557823, 8759840 16932747 19850612 16091051 21383089 22111992 24224907</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

← Older revision		Revision as of 09:44, 17 April 2014
Line 131:		Line 131:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium): 237 {{PubMed\|24696501}}
		+	** number of protein molecules per cell (complex medium with amino acids, without glucose): 743 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

@@ Line 62: / Line 62: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fapR-plsX-fabDG.html]
@@ Line 100: / Line 101: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1VI1 1VI1]

@@ Line 57: / Line 57: @@
 ===Phenotypes of a mutant ===
-** essential {{PubMed|24224907,12682299}}
+* essential {{PubMed|24224907,12682299}}
-** depletion results in sporulation deficiency (via [[SpoIIR]]) {{PubMed|22111992}}
+* depletion results in sporulation deficiency (via [[SpoIIR]]) {{PubMed|22111992}}
-** a ts mutant forms an abberant Z-ring and has a [[cell division]] defect {{PubMed|24224907}}
+* a ts mutant forms an abberant Z-ring and has a [[cell division]] defect {{PubMed|24224907}}
 === Database entries ===

@@ Line 1: / Line 1: @@
-* '''Description:''' acyl-acyl carrier protein (ACP):phosphate acyltransferase, catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4) <br/><br/>
+* '''Description:''' acyl-acyl carrier protein (ACP):phosphate acyltransferase, catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4), coordinates membrane synthesis with [[cell division]] <br/><br/>
 {| align="right" border="1" cellpadding="2"
@@ Line 8: / Line 8: @@
 |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylpD ''
 |-
-|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
+|style="background:#ABCDEF;" align="center"| '''Essential''' || yes {{PubMed|24224907,12682299}}
 |-
 |style="background:#ABCDEF;" align="center"| '''Product''' || acyl-acyl carrier protein (ACP):phosphate acyltransferase
@@ Line 39: / Line 39: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
-<br/><br/><br/><br/>
+<br/><br/>
 = [[Categories]] containing this gene/protein =
 {{SubtiWiki category|[[biosynthesis of lipids]]}},
 {{SubtiWiki category|[[essential genes]]}}
@@ Line 60: / Line 57: @@
 ===Phenotypes of a mutant ===
-** essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
+** essential {{PubMed|24224907,12682299}}
 ** depletion results in sporulation deficiency (via [[SpoIIR]]) {{PubMed|22111992}}
 === Database entries ===
@@ Line 86: / Line 85: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
 * '''[[SubtInteract|Interactions]]:'''
-** [[PlsX]]-[[PlsY]]  {{PubMed|19282621}}
+** [[PlsX]]-[[PlsY]] {{PubMed|19282621}}
 * '''[[Localization]]:'''
 === Database entries ===
@@ Line 117: / Line 118: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=plsX_1662547_1663548_1 plsX] {{PubMed|22383849}}
-* '''Sigma factor:''' [[SigA]] {{PubMed|16932747}}
+* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|16932747}}
 * '''Regulation:'''
@@ Line 149: / Line 150: @@
 =References=
-'''Additional publications:''' {{PubMed|22111992}}
+<pubmed>12737802, 19282621, 17645809, 17557823, 8759840 16932747 19850612 16091051 21383089 22111992 24224907</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 18: / Line 18: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PlsX PlsX]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=plsX plsX]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 5.492

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15890 plsX]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PlsX PlsX]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PlsX PlsX]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''

@@ Line 31: / Line 31: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=plsX_1662547_1663548_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:plsX_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=plsX_1662547_1663548_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:plsX_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15890]]
 |-
 |}

@@ Line 26: / Line 26: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fapR]]'', ''[[fabD]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15890 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15890 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15890 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15890 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15890 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15890 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:plsX_context.gif]]