PhoR - Revision history

Jstuelk at 16:55, 11 February 2015

2015-02-11T16:55:45Z

Jstuelk: /* References */

2014-10-31T12:20:11Z

‎References

Jstuelk: /* The protein */

2014-10-27T15:40:01Z

‎The protein

Jstuelk: /* The protein */

2014-10-27T15:03:00Z

‎The protein

Jstuelk: /* Extended information on the protein */

2014-10-27T14:59:30Z

‎Extended information on the protein

Jstuelk at 14:55, 27 October 2014

2014-10-27T14:55:27Z

Jstuelk: /* References */

2014-10-27T14:19:31Z

‎References

134.76.70.252 at 10:09, 17 April 2014

2014-04-17T10:09:13Z

134.76.38.147 at 14:29, 2 April 2014

2014-04-02T14:29:28Z

134.76.70.252 at 09:06, 12 November 2013

2013-11-12T09:06:28Z

← Older revision		Revision as of 16:55, 11 February 2015
Line 1:		Line 1:
−	* '''Description:~~''' [[two-component systems\|two-component]] sensor kinase, regulation of phosphate metabolism <br/><br/>~~	+	{{#bank: PhoR}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''phoR''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| [[two-component systems\|two-component]] sensor kinase~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| regulation of [[phosphate metabolism]]~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29100 phoR]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PhoR PhoR]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 64 kDa, 5.957~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1737 bp, 579 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[polA]]'', ''[[phoP]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29100 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29100 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29100 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:phoR_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=phoR_2976068_2977807_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:phoR_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29100]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[phosphate metabolism]]}},~~
−	~~{{SubtiWiki category\|[[protein modification]]}},~~
−	~~{{SubtiWiki category\|[[transcription factors and their control]]}},~~
−	~~{{SubtiWiki category\|[[sporulation proteins]]}},~~
−	~~{{SubtiWiki category\|[[general stress proteins (controlled by SigB)]]}},~~
−	~~{{SubtiWiki category\|[[membrane proteins]]}},~~
−	~~{{SubtiWiki category\|[[phosphoproteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[CcpA regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[PhoP regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[SigB regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[SigE regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU29100
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29100&redirect=T BSU29100]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/phoPR.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10364]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' autophosphorylation, phosphorylation of [[PhoP]]
−
−	* '''Protein family:'''
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−	** two transmembrane segments
−	** [[PAS domain]], for binding of an intermediate of wall teichoic acid biosynthesis {{PubMed\|25315493}}
−	** C-terminal histidine phosphotransferase domain
−
−	* '''Modification:'''
−	** autophosphorylation on a His residue in response to the the availability of an intermediate of wall teichoic acid bioynthesis, autophosphorylation is prevented by binding of this intermediate to the intracellular [[PAS domain]] of [[PhoR]] {{PubMed\|25315493}}
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−	** activity is inhibited by binding of an intermediate of wall teichoic acid biosynthesis to the intracellular [[PAS domain]] of [[PhoR]] {{PubMed\|25315493}}
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[PhoP]]-[[PhoR]]
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29100&redirect=T BSU29100]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3CWF 3CWF] (extracellular domain) {{PubMed\|20008068}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P23545 P23545]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29100]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−	* '''Operon:''' ''[[phoP]]-[[phoR]]'' {{PubMed\|14762014}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=phoR_2976068_2977807_-1 phoR] {{PubMed\|22383849}}
−
−	* '''Sigma factor:''' [[SigA]] {{PubMed\|16452408}}, [[SigB]] {{PubMed\|15205429}}, [[SigE]] {{PubMed\|15699190,15205429}}
−
−	* '''Regulation:'''
−	** carbon catabolite repression ([[CcpA]]) {{PubMed\|16452408}}
−	** expressed under conditions of phosphate limitation ([[PhoP]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/15205429,14762014 PubMed]
−	** expressed in post-exponential phase ([[ScoC]]) {{PubMed\|20382764}}
−
−	* '''Regulatory mechanism:'''
−	** [[CcpA]]: transcription repression {{PubMed\|16452408,12850135}}
−	** [[PhoP]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/15205429,14762014 PubMed]
−	** [[ScoC]]: transcription repression {{PubMed\|20382764}}
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 453 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** 1A966 ( ''phoR''::''tet''), {{PubMed\|14973033}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A966&Search=1A966 BGSC]
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−	~~[[Marion Hulett]], University of Illinois at Chicago, USA [http://www.uic.edu/depts/bios/faculty/hulett/hulettm.shtml Homepage]~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~== Reviews ==~~
−	~~<pubmed> 10094672, 25355628 </pubmed>~~
−	~~== Original publications ==~~
−	~~<pubmed>20008068 10433720,15205429,9084179,17085571,9987123,10913081,16452408 20167622 ,15205429,14762014, 20382764 25315493</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 157: / Line 157: @@
 =References=
+== Reviews ==
-<pubmed>20008068 10433720,15205429,9084179,17085571,9987123,10913081,10094672,16452408 20167622 ,15205429,14762014, 20382764 25315493</pubmed>
+<pubmed> 10094672, 25355628 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 85: / Line 85: @@
 * '''Kinetic information:'''
-* '''Domains:''' two transmembrane segments, C-terminal histidine phosphotransferase domain
+* '''[[Domains]]:'''
 * '''Modification:'''
@@ Line 93: / Line 96: @@
 * '''Effectors of protein activity:'''
 * '''[[SubtInteract|Interactions]]:'''

@@ Line 87: / Line 87: @@
 * '''Domains:''' two transmembrane segments, C-terminal histidine phosphotransferase domain
-* '''Modification:''' autophosphorylation on a His residue in response to the the availability of an intermediate of wall teichoic acid bioynthesis, autophosphorylation is prevented by binding of this intermediate to the intracellular [[PAS domain]] of [[PhoR]] {{PubMed|25315493}}
+* '''Modification:'''
 * '''Cofactor(s):'''

@@ Line 87: / Line 87: @@
 * '''Domains:''' two transmembrane segments, C-terminal histidine phosphotransferase domain
-* '''Modification:''' autophosphorylation on a His residue in response to the the availability of an intermediate of wall teichoic acid bioynthesis, autophosphorylation is prevented by binding of this intermediate to the intracellular [[PAS domain]] of [[PhoR]] {{PubMed 25315493}}
+* '''Modification:''' autophosphorylation on a His residue in response to the the availability of an intermediate of wall teichoic acid bioynthesis, autophosphorylation is prevented by binding of this intermediate to the intracellular [[PAS domain]] of [[PhoR]] {{PubMed|25315493}}
 * '''Cofactor(s):'''

@@ Line 37: / Line 37: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/>
@@ Line 74: / Line 70: @@
 === Additional information===
 =The protein=
@@ Line 94: / Line 87: @@
 * '''Domains:''' two transmembrane segments, C-terminal histidine phosphotransferase domain
-* '''Modification:''' autophosphorylation on a His residue
+* '''Modification:''' autophosphorylation on a His residue in response to the the availability of an intermediate of wall teichoic acid bioynthesis, autophosphorylation is prevented by binding of this intermediate to the intracellular [[PAS domain]] of [[PhoR]] {{PubMed 25315493}}
 * '''Cofactor(s):'''
@@ Line 100: / Line 93: @@
 * '''Effectors of protein activity:'''
-* '''[[SubtInteract|Interactions]]:''' [[PhoP]]-[[PhoR]]
+* '''[[SubtInteract|Interactions]]:'''
 * '''[[Localization]]:'''
@@ Line 107: / Line 101: @@
 * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29100&redirect=T BSU29100]
-* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3CWF 3CWF]	(extracellular PAS domain)  {{PubMed|20008068}}
+* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3CWF 3CWF] (extracellular domain)  {{PubMed|20008068}}
 * '''UniProt:''' [http://www.uniprot.org/uniprot/P23545 P23545]
@@ Line 159: / Line 153: @@
 =References=
-<pubmed>20008068 10433720,15205429,9084179,17085571,9987123,10913081,10094672,16452408 20167622 ,15205429,14762014, 20382764 25315493 </pubmed>
+<pubmed>20008068 10433720,15205429,9084179,17085571,9987123,10913081,10094672,16452408 20167622 ,15205429,14762014, 20382764 25315493</pubmed>
 [[Category:Protein-coding genes]]

← Older revision		Revision as of 10:09, 17 April 2014
Line 135:		Line 135:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (complex medium with amino acids, without glucose): 453 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

@@ Line 67: / Line 67: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/phoPR.html]
@@ Line 104: / Line 105: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3CWF 3CWF]	(extracellular PAS domain)  {{PubMed|20008068}}

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29100 phoR]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PhoR PhoR]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PhoR PhoR]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 64 kDa, 5.957