PbpB - Revision history

Jstuelk at 12:12, 11 December 2014

2014-12-11T12:12:13Z

Jgundlach at 15:54, 15 August 2014

2014-08-15T15:54:34Z

134.76.38.147 at 13:38, 2 April 2014

2014-04-02T13:38:50Z

Jstuelk: /* References */

2014-03-25T08:03:11Z

‎References

Jstuelk at 08:54, 5 March 2014

2014-03-05T08:54:59Z

Jstuelk: /* Database entries */

2014-01-29T15:48:03Z

‎Database entries

Jstuelk at 15:47, 29 January 2014

2014-01-29T15:47:45Z

Jstuelk: /* References */

2014-01-29T15:17:17Z

‎References

Jstuelk: /* Extended information on the protein */

2014-01-29T14:41:46Z

‎Extended information on the protein

Jstuelk at 14:33, 29 January 2014

2014-01-29T14:33:36Z

← Older revision		Revision as of 12:12, 11 December 2014
Line 1:		Line 1:
−	* '''Description:~~''' class B [[penicillin-binding protein]] 2B, Pbp2B <br/><br/>~~	+	{{#bank: PbpB}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''pbpB''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| [[penicillin-binding protein]] 2B~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| septation, [[cell division]]~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15160 pbpB]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PbpB PbpB]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 79 kDa, 9.234~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 2148 bp, 716 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[ftsL]]'', ''[[spoVD]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15160 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15160 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15160 DNA_with_flanks]
−	\|-
−	\|-
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:pbpB_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pbpB_1581947_1584097_1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:pbpB_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15160]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[cell wall synthesis]]}},~~
−	~~{{SubtiWiki category\|[[cell division]]}},~~
−	~~{{SubtiWiki category\|[[essential genes]]}},~~
−	~~{{SubtiWiki category\|[[membrane proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU15160
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15160&redirect=T BSU15160]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yllB-ylxA-ftsL-pbpB.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10221]
−
−	~~=== Additional information===~~
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−
−	* '''Protein family:''' transpeptidase family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):''' [[SpoVD]]
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** folding requires [[PrsA]] {{PubMed\|20487272}}
−	** [[PbpB]]-[[DivIB]]-([[FtsL]]-[[DivIC]])2 {{PubMed\|20870765}}, as part of the [[divisome]]
−	** [[WalK]]-[[PbpB]] {{PubMed\|21219466}}
−
−	* '''[[Localization]]:'''
−	** cell membrane {{PubMed\|23869552,20870765}}
−	** division sites {{PubMed\|23895585}}
−	** extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
−	** localizes to the sporulation septum during sporulation [http://www.ncbi.nlm.nih.gov/sites/entrez/15758244 PubMed]
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15160&redirect=T BSU15160]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1rp5 1RP5] (PBP 2x from ''Streptococcus pneumoniae'', 32% identity) {{PubMed\|14734544}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/Q07868 Q07868]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15160]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[mraZ]]-[[mraW]]-[[ftsL]]-[[pbpB]]'' {{PubMed\|8636036,8244929}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pbpB_1581947_1584097_1 pbpB] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−
−	* '''Regulation:'''
−	** constitutively expressed [http://www.ncbi.nlm.nih.gov/sites/entrez/15758244 PubMed]
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jeff Errington]] lab
−
−	* '''FLAG-tag construct:'''
−	** GP2003 ''pbpB-3xFLAG spec'' (based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~[[Jeff Errington]], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~<pubmed>8636036,14731276,8244929,20487272,10652091, 19429628, 18957862 21219466 20870765 14734544 23895585 24655324 </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

← Older revision		Revision as of 15:54, 15 August 2014
Line 142:		Line 142:

	* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jeff Errington]] lab		* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jeff Errington]] lab
		+
		+	* '''FLAG-tag construct:'''
		+	** GP2003 ''pbpB-3xFLAG spec'' (based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab

	* '''Antibody:'''		* '''Antibody:'''

@@ Line 61: / Line 61: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yllB-ylxA-ftsL-pbpB.html]
@@ Line 103: / Line 104: @@
 === Database entries ===
 * '''Structure:'''	[http://www.rcsb.org/pdb/explore/explore.do?structureId=1rp5 1RP5] (PBP 2x from ''Streptococcus pneumoniae'', 32% identity) {{PubMed|14734544}}

@@ Line 150: / Line 150: @@
 =References=
-<pubmed>8636036,14731276,8244929,20487272,10652091, 19429628, 18957862 21219466 20870765 14734544 23895585 </pubmed>
+<pubmed>8636036,14731276,8244929,20487272,10652091, 19429628, 18957862 21219466 20870765 14734544 23895585 24655324 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 1: / Line 1: @@
-* '''Description:''' [[penicillin-binding protein]]  2B, Pbp2B <br/><br/>
+* '''Description:''' class B [[penicillin-binding protein]]  2B, Pbp2B <br/><br/>
 {| align="right" border="1" cellpadding="2"
@@ Line 12: / Line 12: @@
 |style="background:#ABCDEF;" align="center"| '''Product''' || [[penicillin-binding protein]]  2B
 |-
-|style="background:#ABCDEF;" align="center"|'''Function''' || septation
+|style="background:#ABCDEF;" align="center"|'''Function''' || septation, [[cell division]]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15160 pbpB]
@@ Line 43: / Line 43: @@
 = [[Categories]] containing this gene/protein =
 {{SubtiWiki category|[[cell wall synthesis]]}},
 {{SubtiWiki category|[[essential genes]]}},
 {{SubtiWiki category|[[membrane proteins]]}}
@@ Line 97: / Line 98: @@
 * '''[[Localization]]:'''
 ** cell membrane {{PubMed|23869552,20870765}}
-**  extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
+** division sites {{PubMed|23895585}}
 ** localizes to the sporulation septum during sporulation [http://www.ncbi.nlm.nih.gov/sites/entrez/15758244 PubMed]
@@ Line 148: / Line 150: @@
 =References=
-<pubmed>8636036,14731276,8244929,20487272,10652091, 19429628, 18957862 21219466 20870765 14734544 </pubmed>
+<pubmed>8636036,14731276,8244929,20487272,10652091, 19429628, 18957862 21219466 20870765 14734544 23895585 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 102: / Line 102: @@
 === Database entries ===
-* '''Structure:'''	[http://www.rcsb.org/pdb/explore/explore.do?structureId=1rp5 1RP5] (PBP 2x from [[Streptococcus pneumoniae]], 32% identity) {{PubMed|14734544}}
+* '''Structure:'''	[http://www.rcsb.org/pdb/explore/explore.do?structureId=1rp5 1RP5] (PBP 2x from ''Streptococcus pneumoniae'', 32% identity) {{PubMed|14734544}}
 * '''UniProt:''' [http://www.uniprot.org/uniprot/Q07868 Q07868]

@@ Line 148: / Line 148: @@
 =References=
-<pubmed>8636036,14731276,8244929,20487272,10652091, 19429628, 18957862 21219466 16950129 20870765</pubmed>
+<pubmed>8636036,14731276,8244929,20487272,10652091, 19429628, 18957862 21219466 20870765</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 94: / Line 94: @@
 ** [[PbpB]]-[[DivIB]]-([[FtsL]]-[[DivIC]])2 {{PubMed|20870765}}, as part of the [[divisome]]
 ** [[WalK]]-[[PbpB]] {{PubMed|21219466}}
 * '''[[Localization]]:'''