ParB - Revision history

Jstuelk at 08:59, 24 August 2015

2015-08-24T08:59:13Z

Jstuelk: /* Original publications */

2015-08-24T08:53:28Z

‎Original publications

Jstuelk: /* Database entries */

2015-08-14T13:57:50Z

‎Database entries

Jstuelk: /* Expression and regulation */

2015-08-13T08:10:36Z

‎Expression and regulation

Jstuelk: /* Original publications */

2015-08-10T08:32:17Z

‎Original publications

Jstuelk at 08:27, 10 August 2015

2015-08-10T08:27:02Z

Jstuelk at 08:03, 10 August 2015

2015-08-10T08:03:35Z

Jstuelk at 08:03, 10 August 2015

2015-08-10T08:03:15Z

Jstuelk: Jstuelk moved page Spo0J to ParB: 16677298

2015-08-10T08:00:27Z

Jstuelk moved page Spo0J to ParB: 16677298

Jstuelk: /* Original publications */

2015-05-08T07:57:18Z

‎Original publications

← Older revision		Revision as of 08:59, 24 August 2015
Line 1:		Line 1:
−	* '''Description:''' chromosome positioning near the pole and transport through the polar septum / antagonist of [[ParA]]-dependent inhibition of [[sporulation]] initiation, traps DNA loops, required for the recruitment of the [[condensin\|SMC complex]] to the origin of replication <br/><br/>	+	{{#bank: ParB}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''parB''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''spo0J, spo0JB ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| site-specific DNA-binding protein~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| chromosome positioning before asymmetric septation,~~
−
−	~~specifying its orientation, and imposing directionality on~~
−
−	~~its subsequent transport through the septum; required for sporulation~~
−
−	~~(antagonist of [[Soj]]-dependent inhibition of sporulation initiation)~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40960 parB]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=ParB ParB]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 32 kDa, 8.417~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 846 bp, 282 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[yyaC]]'', ''[[parA]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU40960 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU40960 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU40960 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:spo0J_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=parB_4205556_4206404_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:spo0J_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40960]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[DNA condensation/ segregation]]}},~~
−	~~{{SubtiWiki category\|[[sporulation/ other]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[Spo0A regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU40960
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU40960&redirect=T BSU40960]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/soj-spo0J.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10054]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−	** inhibits [[ParA]] dimerization and concomitant DNA-binding activity by stimulating [[ParA]] ATPase activity {{PubMed\|21235642}}
−	** recruits the condensin complex to the DNA origin region {{PubMed\|24440393}}
−	** the dimer binds specifcally to the centromere-like ''parS'' sequence {{PubMed\|25572315}}
−
−	* '''Protein family:''' parB family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):''' [[Noc]]
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** forms dimers {{PubMed\|24829297}}
−	** [[ParA]]-[[ParB]] {{PubMed\|21235642}}
−
−	* '''[[Localization]]:'''
−	** chromosome centromer {{PubMed\|23475963}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU40960&redirect=T BSU40960]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=4UMK 4UMK] (complex with ''parS'' DNA)
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P26497 P26497]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU40960]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−	* '''Operon:''' ''[[parA]]-[[parB]]'' {{PubMed\|22383849}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=parB_4205556_4206404_-1 parB] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−
−	* '''Regulation:'''
−	** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
−
−	* '''Regulatory mechanism:'''
−	** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 341 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 362 {{PubMed\|24696501}}
−	** number of protein molecules per cell: 880 (equivalent to 440 dimers) {{PubMed\|24829297}}
−
−	~~=Biological materials =~~
−	* '''Mutant:'''
−	** 1S135 ( ''spo0J''::''spec''), {{PubMed\| }}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S135&Search=1S135 BGSC]
−	** 1S136 ( ''spo0J''::''spec''), {{PubMed\| }}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S136&Search=1S136 BGSC]
−	** 1S137 ( ''spo0J''::''spec''), {{PubMed\| }}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S137&Search=1S137 BGSC]
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−	* [[Heath Murray]], Centre for Bacterial Cell Biology, Newcastle, UK [http://www.ncl.ac.uk/camb/staff/profile/heath.murray homepage]
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~== Reviews ==~~
−	~~<pubmed> 22934648 </pubmed>~~
−	~~== Original publications ==~~
−	<pubmed>17462018 16677298 12562803, 10852876, 10482533, 9506522, 8071208, 17932079 19450517 21235642 26253537 9663676, 11532141, 19450516,15659156, 18854156,9159397,8866474,9778525,16925562,1900505,9364919,9701805,12950914,,14651647, 19450517, 19450516, 14563866 23475963 21911367 24440393 24829297 24696501 25071173 25572315 25951515 26295962</pubmed>
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 157: / Line 157: @@
 <pubmed> 22934648 </pubmed>
 == Original publications ==
-<pubmed>17462018 16677298 12562803, 10852876, 10482533, 9506522, 8071208, 17932079 19450517 21235642 26253537 9663676, 11532141, 19450516,15659156, 18854156,9159397,8866474,9778525,16925562,1900505,9364919,9701805,12950914,,14651647, 19450517, 19450516, 14563866 23475963 21911367 24440393 24829297 24696501 25071173 25572315 25951515</pubmed>
+<pubmed>17462018 16677298 12562803, 10852876, 10482533, 9506522, 8071208, 17932079 19450517 21235642 26253537 9663676, 11532141, 19450516,15659156, 18854156,9159397,8866474,9778525,16925562,1900505,9364919,9701805,12950914,,14651647, 19450517, 19450516, 14563866 23475963 21911367 24440393 24829297 24696501 25071173 25572315 25951515 26295962</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 104: / Line 104: @@
 * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU40960&redirect=T BSU40960]
-* '''Structure:'''
+* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=4UMK 4UMK] (complex with ''parS'' DNA)
 * '''UniProt:''' [http://www.uniprot.org/uniprot/P26497 P26497]

@@ Line 115: / Line 115: @@
 =Expression and regulation=
-* '''Operon:''' ''[[soj]]-[[spo0J]]'' {{PubMed|22383849}}
+* '''Operon:''' ''[[parA]]-[[parB]]'' {{PubMed|22383849}}
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=parB_4205556_4206404_-1 parB] {{PubMed|22383849}}

@@ Line 157: / Line 157: @@
 <pubmed> 22934648 </pubmed>
 == Original publications ==
-<pubmed>17462018 16677298 12562803, 10852876, 10482533, 9506522, 8071208, 17932079 19450517 21235642 9663676,11532141,19450516,15659156,18854156,9159397,8866474,9778525,16925562,1900505,9364919,9701805,12950914,,14651647, 19450517, 19450516, 14563866 23475963 21911367 24440393 24829297 24696501 25071173 25572315 25951515</pubmed>
+<pubmed>17462018 16677298 12562803, 10852876, 10482533, 9506522, 8071208, 17932079 19450517 21235642 26253537 9663676, 11532141, 19450516,15659156, 18854156,9159397,8866474,9778525,16925562,1900505,9364919,9701805,12950914,,14651647, 19450517, 19450516, 14563866 23475963 21911367 24440393 24829297 24696501 25071173 25572315 25951515</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 22: / Line 22: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40960 parB]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=Spo0J Spo0J]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=ParB ParB]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 8.417

← Older revision		Revision as of 08:03, 10 August 2015
Line 1:		Line 1:
−	* '''Description:''' chromosome positioning near the pole and transport through the polar septum / antagonist of [[~~Soj~~]]-dependent inhibition of [[sporulation]] initiation, traps DNA loops, required for the recruitment of the [[condensin\|SMC complex]] to the origin of replication <br/><br/>	+	* '''Description:''' chromosome positioning near the pole and transport through the polar septum / antagonist of [[ParA]]-dependent inhibition of [[sporulation]] initiation, traps DNA loops, required for the recruitment of the [[condensin\|SMC complex]] to the origin of replication <br/><br/>

	{\| align="right" border="1" cellpadding="2"		{\| align="right" border="1" cellpadding="2"

@@ Line 4: / Line 4: @@
 |-
 |style="background:#ABCDEF;" align="center"|'''Gene name'''
-|''spo0J''
+|''parB''
 |-
-|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''parB, spo0JB ''
+|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''spo0J, spo0JB ''
 |-
 |style="background:#ABCDEF;" align="center"| '''Essential''' || no
@@ Line 20: / Line 20: @@
 (antagonist of [[Soj]]-dependent inhibition of sporulation initiation)
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40960 spo0J]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40960 parB]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=Spo0J Spo0J]
@@ Line 28: / Line 28: @@
 |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 846 bp, 282 aa
 |-
-|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yyaC]]'', ''[[soj]]''
+|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yyaC]]'', ''[[parA]]''
 |-
 |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU40960 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU40960 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU40960 DNA_with_flanks]
@@ Line 74: / Line 74: @@
 * '''Catalyzed reaction/ biological activity:'''
-** inhibits [[Soj]] dimerization and concomitant DNA-binding activity by stimulating Soj ATPase activity {{PubMed|21235642}}
+** inhibits [[ParA]] dimerization and concomitant DNA-binding activity by stimulating [[ParA]] ATPase activity {{PubMed|21235642}}
 ** recruits the condensin complex to the DNA origin region {{PubMed|24440393}}
 ** the dimer binds specifcally to the centromere-like ''parS'' sequence {{PubMed|25572315}}
@@ Line 96: / Line 96: @@
 * '''[[SubtInteract|Interactions]]:'''
 ** forms dimers {{PubMed|24829297}}
-** [[Spo0J]]-[[Soj]] {{PubMed|21235642}}
+** [[ParA]]-[[ParB]] {{PubMed|21235642}}
 * '''[[Localization]]:'''
@@ Line 117: / Line 117: @@
 * '''Operon:''' ''[[soj]]-[[spo0J]]'' {{PubMed|22383849}}
-* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=parB_4205556_4206404_-1 spo0J] {{PubMed|22383849}}
+* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=parB_4205556_4206404_-1 parB] {{PubMed|22383849}}
 * '''[[Sigma factor]]:'''
@@ Line 131: / Line 131: @@
 ** number of protein molecules per cell (complex medium with amino acids, without glucose): 362 {{PubMed|24696501}}
 ** number of protein molecules per cell: 880 (equivalent to 440 dimers) {{PubMed|24829297}}
 =Biological materials =
 * '''Mutant:'''

← Older revision	Revision as of 08:00, 10 August 2015
(No difference)