PTTB1 - Revision history

Bzhu at 11:36, 28 August 2017

2017-08-28T11:36:17Z

Bzhu at 09:26, 28 August 2017

2017-08-28T09:26:53Z

Bzhu: Created page with " {{ContentDisclamer}} 600px 230px The vector '''pTTB1''' is a low copy '''food grade''' expression vector for constitutive expression...."

2017-08-21T13:54:55Z

Created page with " {{ContentDisclamer}} 600px 230px The vector '''pTTB1''' is a low copy '''food grade''' expression vector for constitutive expression...."

New page

{{ContentDisclamer}}

[[File:PTTB1.PNG|600px]]
[[File:Legend.png|230px]]

The vector '''pTTB1''' is a low copy '''food grade''' expression vector for constitutive expression. Selection in ''B. subtilis'' is based on the interplay of the endogenous ''Bacillus'' toxin EndoA (encoded within the genome of host strains ''B. subtilis'' TEA or ''B. subtilis'' WEA) and its antitoxin EndoB (vector encoded).
For easier handling pTTB1 is designed as ''B. subtilis / E. coli'' shuttle vector. The parts of the vector used for cloning with ''E. coli'' (''E. coli'' origin and ampicillin resistance cassette) can be eliminated afterwards by restriction enzyme cleavage and religation of the vector. This technique connects the advantage of easy cloning with ''E. coli'' with the food grade property of ''B. subtilis''.

=== Features ===
* Food grade protein production
* Stable low level expression without addition of any antibiotics
* All DNA contained in the final expression system is derived from ''B. subtilis''
* No endotoxins are produced
* Corresponding protease-deficient strain for secretory protein production is available

The '''pTTB1''' vector and corresponding strains B. subtilis TEA and B. subtilis WEA are available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)]

==Reference==

<pubmed>26721182</pubmed>

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 * Corresponding protease-deficient strain for secretory protein production is available
-The '''pTTB1''' vector and corresponding strains B. subtilis TEA and B. subtilis WEA are available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)]
+The '''pTTB1''' vector and corresponding strains B. subtilis TEA and B. subtilis WEA are available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)][[File:Mobiteclogo.png|100px]]
 ==Reference==
 <pubmed>26721182</pubmed>

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 [[File:PTTB1.PNG|600px]]
-[[File:Legend.png|230px]]
+[[File:PTTB1-Legende.jpg|300px]]
 The vector '''pTTB1''' is a low copy '''food grade''' expression vector for constitutive expression. Selection in ''B. subtilis'' is based on the interplay of the endogenous ''Bacillus'' toxin EndoA (encoded within the genome of host strains ''B. subtilis'' TEA or ''B. subtilis'' WEA) and its antitoxin EndoB (vector encoded).