PHT01 - Revision history

Bzhu at 11:34, 28 August 2017

2017-08-28T11:34:29Z

2017-08-28T09:21:13Z

2017-08-21T13:00:58Z

2017-08-21T12:38:18Z

2017-08-21T12:35:30Z

2017-08-21T12:32:46Z

2017-08-21T12:32:17Z

2017-08-21T12:30:41Z

2017-08-21T12:29:30Z

‎Features

2017-08-21T12:22:15Z

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 * ''E. coli / B. subtilis'' shuttle vector
-pHT01 and further pHT-vector variants (e.g., with His, Strep or Myc tag)  are available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)]
+pHT01 and further pHT-vector variants (e.g., with His, Strep or Myc tag)  are available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)][[File:Mobiteclogo.png|100px]]
 == References ==
 <pubmed>17624574, 16005967, 16125412</pubmed>

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 [[File:PHT01.PNG|600px]]
-[[File:Legend.png|230px]]
+[[File:PHT01-Legende.jpg|300px]]
 The pHT01 is a high-level intracellular expression vector for recombinant protein production with <i>B. subtilis</i>. The vector is based on the strong σ<sup>A</sup>-dependent promoter preceding the ''[[groES]]-[[groEL]]'' operon of <i>B. subtilis</i> which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. pHT01 is an <i>E. coli/ B. subtilis</i> shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis.

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 [[File:Legend.png|230px]]
-The pHT01 is a high-level intracellular expression vector for recombinant protein production with <i>B. subtilis</i>. The vector is based on the strong σ<sup>A</sup>-dependent promoter preceding the groE operon of <i>B. subtilis</i> which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. pHT01 is an <i>E. coli/ B. subtilis</i> shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis.
+The pHT01 is a high-level intracellular expression vector for recombinant protein production with <i>B. subtilis</i>. The vector is based on the strong σ<sup>A</sup>-dependent promoter preceding the ''[[groES]]-[[groEL]]'' operon of <i>B. subtilis</i> which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. pHT01 is an <i>E. coli/ B. subtilis</i> shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis.
 === Features ===

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 {{ContentDisclamer}}
-[[File:PHT01.PNG|500px]]
+[[File:PHT01.PNG|600px]]
 The pHT01 is a high-level intracellular expression vector for recombinant protein production with <i>B. subtilis</i>. The vector is based on the strong σ<sup>A</sup>-dependent promoter preceding the groE operon of <i>B. subtilis</i> which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. pHT01 is an <i>E. coli/ B. subtilis</i> shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis.

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 {{ContentDisclamer}}
-[[File:PHT01.PNG|300px|thumb|left]]
+[[File:PHT01.PNG|500px]]
 The pHT01 is a high-level intracellular expression vector for recombinant protein production with <i>B. subtilis</i>. The vector is based on the strong σ<sup>A</sup>-dependent promoter preceding the groE operon of <i>B. subtilis</i> which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. pHT01 is an <i>E. coli/ B. subtilis</i> shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis.

← Older revision		Revision as of 12:30, 21 August 2017
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	{{ContentDisclamer}}		{{ContentDisclamer}}
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		+	[[File:PHT01.PNG]]

	The pHT01 is a high-level intracellular expression vector for recombinant protein production with <i>B. subtilis</i>. The vector is based on the strong σ<sup>A</sup>-dependent promoter preceding the groE operon of <i>B. subtilis</i> which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. pHT01 is an <i>E. coli/ B. subtilis</i> shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis.		The pHT01 is a high-level intracellular expression vector for recombinant protein production with <i>B. subtilis</i>. The vector is based on the strong σ<sup>A</sup>-dependent promoter preceding the groE operon of <i>B. subtilis</i> which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. pHT01 is an <i>E. coli/ B. subtilis</i> shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis.

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 The pHT01 is a high-level intracellular expression vector for recombinant protein production with <i>B. subtilis</i>. The vector is based on the strong σ<sup>A</sup>-dependent promoter preceding the groE operon of <i>B. subtilis</i> which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. pHT01 is an <i>E. coli/ B. subtilis</i> shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis.
-<h2>Features</h2>
+== Features ==
 * Efficiently controllable gene expression (IPTG inducible)
 * High level production of recombinant proteins
 * ''E. coli / B. subtilis'' shuttle vector