PGP888 - Revision history

Jstuelk at 13:59, 3 June 2013

2013-06-03T13:59:38Z

Jstuelk at 14:27, 16 August 2012

2012-08-16T14:27:04Z

Jstuelk: /* The reference */

2011-10-31T15:40:06Z

‎The reference

Jstuelk at 07:04, 28 August 2011

2011-08-28T07:04:38Z

Kgunka at 11:44, 26 August 2011

2011-08-26T11:44:44Z

Kgunka at 11:44, 26 August 2011

2011-08-26T11:44:11Z

Kgunka: Created page with "'''pGP888: click to enlarge''' The vector was constructed in the Stülke lab and it is suitable for the xylose-inducible expression of ..."

2011-08-26T11:43:09Z

Created page with "250px|thumb|right|'''pGP888: click to enlarge''' The vector was constructed in the Stülke lab and it is suitable for the xylose-inducible expression of ..."

New page

[[File:PGP888.png|250px|thumb|right|'''pGP888: click to enlarge''']]

The vector was constructed in the [[Stülke]] lab and it is suitable for the xylose-inducible expression of proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI or NotI

''' Hints for usage'''
*An additional base has to be inserted in the forward primer to restore the frame.
*Don't forget a stop codon in the reverse primer.

<pubmed>21856853</pubmed>

@@ Line 7: / Line 7: @@
 *An additional base has to be inserted in the forward primer to restore the frame.
 *Don't forget a stop codon in the reverse primer.
 == The reference ==
- <big>''Diethmaier C, Pietack N, Gunka K, Wrede C, Lehnik-Habrink M, Herzberg C, Hübner S, Stülke J''  </big>
+<pubmed>21856853 </pubmed>
- <big>'''A Novel Factor Controlling Bistability in ''Bacillus subtilis'': The YmdB Protein Affects</big>
+==  Return to [[Plasmids]] ==

← Older revision		Revision as of 14:27, 16 August 2012
Line 1:		Line 1:
	[[File:pGP888.png\|250px\|thumb\|right\|'''pGP888: click to enlarge''']]		[[File:pGP888.png\|250px\|thumb\|right\|'''pGP888: click to enlarge''']]

−	The vector was constructed in ~~the~~ [[Stülke]] lab and it is suitable for the xylose-inducible expression of proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI or NotI	+	The vector was constructed in [[Jörg Stülke]]'s lab and it is suitable for the xylose-inducible expression of proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI or NotI. The plasmid will integrate into the ''[[ganA]]'' gene.

@@ Line 14: / Line 14: @@
   <big>'''A Novel Factor Controlling Bistability in ''Bacillus subtilis'': The YmdB Protein Affects</big>
   <big>Flagellin Expression and Biofilm Formation.''' </big>
-  J Bacteriol. 2011 Aug 19. [Epub ahead of print]
+  <big>J Bacteriol.: 2011, 193(21):5997-6007. </big>
   [http://www.ncbi.nlm.nih.gov/pubmed/21856853 PubMed:21856853]

← Older revision		Revision as of 07:04, 28 August 2011
Line 10:		Line 10:


−	'''~~The reference~~'''	+	== The reference ==
−	<~~pubmed~~> ~~21856853~~</pubmed>	+	<big>''Diethmaier C, Pietack N, Gunka K, Wrede C, Lehnik-Habrink M, Herzberg C, Hübner S, Stülke J'' </big>
		+	<big>'''A Novel Factor Controlling Bistability in ''Bacillus subtilis'': The YmdB Protein Affects</big>
		+	<big>Flagellin Expression and Biofilm Formation.''' </big>
		+	J Bacteriol. 2011 Aug 19. [Epub ahead of print]
		+	[http://www.ncbi.nlm.nih.gov/pubmed/21856853 PubMed:21856853]

← Older revision		Revision as of 11:44, 26 August 2011
Line 1:		Line 1:
−	[[File:~~PGP888~~.png\|250px\|thumb\|right\|'''pGP888: click to enlarge''']]	+	[[File:pGP888.png\|250px\|thumb\|right\|'''pGP888: click to enlarge''']]

	The vector was constructed in the [[Stülke]] lab and it is suitable for the xylose-inducible expression of proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI or NotI		The vector was constructed in the [[Stülke]] lab and it is suitable for the xylose-inducible expression of proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI or NotI