PGP380 - Revision history

Fabian Commichau at 08:13, 29 July 2014

2014-07-29T08:13:17Z

2013-06-03T13:58:54Z

2013-05-28T10:12:02Z

2010-02-25T11:53:10Z

2010-02-25T11:52:40Z

2010-02-25T11:49:03Z

2010-02-25T11:47:51Z

2009-09-07T18:39:06Z

2009-05-08T13:41:33Z

2009-04-16T11:23:50Z

← Older revision		Revision as of 08:13, 29 July 2014
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	*'''M13_puc_for:''' 5‘-GTAAAACGACGGCCAGTG-3‘		*'''M13_puc_for:''' 5‘-GTAAAACGACGGCCAGTG-3‘
	*'''M13_puc_rev:''' 5‘-GGAAACAGCTATGACCATG-3‘		*'''M13_puc_rev:''' 5‘-GGAAACAGCTATGACCATG-3‘
		+	*'''FX125 (rev; primes -73bp of MCS):''' 5‘-GGCTCGTATGTTGTGTGG-3‘
		+	*'''JN54 (fwd; primes +148bp of MCS):''' 5‘-GTGAAAAATGAGCCGAAAGCAG-3‘

@@ Line 3: / Line 3: @@
 '''Application:'''
-The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive overexpression of N-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and erythromycin in ''E. coli'' and ''B. subtilis'', respectively. pGP380 can be used for the [[SPINE]] method. [[pGP382]] is similar to the vector pGP380 which is derived from vectors [[pBQ200]] and [[pHT315]]. A Shine-Dalgarno sequence has to be fused to the open reading frame during PCR.
+The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive overexpression of N-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and erythromycin in ''E. coli'' and ''B. subtilis'', respectively. pGP380 can be used for the [[SPINE]] method. [[pGP382]] is similar to the vector pGP380 which is derived from vectors [[pBQ200]] and [[pHT315]].
 Sequencing primers:

@@ Line 3: / Line 3: @@
 '''Application:'''
-The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive overexpression of N-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and erythromycin in ''E. coli'' and ''B. subtilis'', respectively. pGP380 can be used for the [[SPINE]] method. [[pGP382]] is similar to the vector pGP380 which is derived from vectors [[pBQ200]] and [[pHT315]].
+The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive overexpression of N-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and erythromycin in ''E. coli'' and ''B. subtilis'', respectively. pGP380 can be used for the [[SPINE]] method. [[pGP382]] is similar to the vector pGP380 which is derived from vectors [[pBQ200]] and [[pHT315]]. A Shine-Dalgarno sequence has to be fused to the open reading frame during PCR.
 Sequencing primers:

@@ Line 1: / Line 1: @@
 [[File:PGP380.jpeg|250px|thumb|right|'''pGP380: click to enlarge''']]
 [[File:PGP380_cloning_region.jpeg|250px|thumb|right|'''pGP380 cloning region: click to enlarge''']]
-The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive overexpression of N-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and erythromycin in ''E. coli'' and ''B. subtilis'', respectively. pGP380 can be used for the [[SPINE]] method. [[pGP382]] is similar to the vector pGP380 which is based on the vectors [[pBQ200]] and [[pHT315]].
+The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive overexpression of N-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and erythromycin in ''E. coli'' and ''B. subtilis'', respectively. pGP380 can be used for the [[SPINE]] method. [[pGP382]] is similar to the vector pGP380 which is derived from vectors [[pBQ200]] and [[pHT315]].
-sequencing primer:
+Sequencing primers:
 *'''M13_puc_for:''' 5‘-GTAAAACGACGGCCAGTG-3‘
 *'''M13_puc_rev:''' 5‘-GGAAACAGCTATGACCATG-3‘

← Older revision		Revision as of 18:39, 7 September 2009
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−	Herzberg, C., Flórez Weidinger, L. A., Dörrbecker, B., Hübner, S., Stülke, J. & Commichau, F. M. (2007) SPINE: A method for the rapid detection and analysis of protein-protein interactions in vivo. Proteomics 7: 4032-4035. [http:/~~/www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17994626 PubMed]~~	+	<pubmed> 17994626 </pubmed>

@@ Line 2: / Line 2: @@
 [[File:PGP380_cloning_region.jpeg|250px|thumb|right|'''pGP380 cloning region: click to enlarge''']]
-Vector for the overexpression of N-terminally Strep-tagged proteins in ''B. subtilis'', used for the SPINE technology
+Vector for the overexpression of N-terminally Strep-tagged proteins in ''B. subtilis'', used for the [[SPINE]] technology
 constructed in [[Stülke]] lab

@@ Line 8: / Line 8: @@
 in ''E. coli'': ampicillin resistance
-in B''. subtilis'': erythromycin resistance
+in ''B. subtilis'': erythromycin resistance
 based on [[pBQ200]]