PGP1871 - Revision history

Dtoedter at 13:23, 2 April 2015

2015-04-02T13:23:47Z

Jstuelk at 17:05, 3 June 2013

2013-06-03T17:05:12Z

Jstuelk at 13:54, 3 June 2013

2013-06-03T13:54:12Z

Jstuelk: moved Pgp1871 to PGP1871

2012-08-01T12:26:30Z

moved Pgp1871 to PGP1871

MLehnik: Created page with "'''pGP1871: click to enlarge''' '''General comments''' Integrative plasmid for the fusion of GFP tag to the C-terminus of a protein, ..."

2011-11-03T12:33:05Z

Created page with "250px|thumb|right|'''pGP1871: click to enlarge''' '''General comments''' Integrative plasmid for the fusion of GFP tag to the C-terminus of a protein, ..."

New page

[[File:PGP1871.png|250px|thumb|right|'''pGP1871: click to enlarge''']]

'''General comments'''

Integrative [[plasmid]] for the fusion of GFP tag to the C-terminus of a protein, keeping the control of the expression under its natural promotor
* ''E.coli'': multicopy plasmid, select for ampicillin
* ''B. subtilis'': no ori, select for spectinomycin

'''Hints for the usage'''
* amplify the last 600bp of your gene of interest,
* skip the Stopp-Codon of the gene in the reverse primer
* take care that the integration is in-frame
* do not use the HindIII site for cloning, as GFP is inserted via this restriction site

← Older revision		Revision as of 17:05, 3 June 2013
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	* do not use the HindIII site for cloning, as GFP is inserted via this restriction site		* do not use the HindIII site for cloning, as GFP is inserted via this restriction site

		+	'''The reference'''
		+	<pubmed> 23475962 </pubmed>
	== Return to [[Plasmids]] ==		== Return to [[Plasmids]] ==

← Older revision		Revision as of 13:54, 3 June 2013
Line 12:		Line 12:
	* take care that the integration is in-frame		* take care that the integration is in-frame
	* do not use the HindIII site for cloning, as GFP is inserted via this restriction site		* do not use the HindIII site for cloning, as GFP is inserted via this restriction site
		+
		+	== Return to [[Plasmids]] ==

@@ Line 3: / Line 3: @@
 '''General comments'''
-Integrative [[plasmid]] for the fusion of GFP tag to the C-terminus of a protein, keeping the control of the expression under its natural promotor
+Integrative [[plasmid]] for the fusion of YFP tag to the C-terminus of a protein, keeping the control of the expression under its natural promotor
 * ''E.coli'': multicopy plasmid, select for ampicillin
 * ''B. subtilis'': no ori, select for spectinomycin

← Older revision	Revision as of 12:26, 1 August 2012
(No difference)