PGP172 - Revision history

Jstuelk at 14:04, 3 June 2013

2013-06-03T14:04:11Z

2013-04-16T06:56:18Z

2013-04-16T06:55:57Z

2010-07-30T07:13:12Z

2010-04-14T10:19:08Z

2010-04-14T10:18:27Z

Undo revision 121171 by Fabian Commichau (Talk)

2010-04-14T10:17:55Z

Undo revision 121172 by Fabian Commichau (Talk)

2010-02-25T14:58:52Z

2010-02-25T14:57:23Z

2009-09-07T18:37:46Z

← Older revision		Revision as of 14:04, 3 June 2013
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	* important: ''Eco''RI, although mentioned in the MCS, is not unique!		* important: ''Eco''RI, although mentioned in the MCS, is not unique!
−

	<pubmed> 14717704 </pubmed>		<pubmed> 14717704 </pubmed>
		+	== Return to [[Plasmids]] ==

@@ Line 19: / Line 19: @@
 ** '''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
-* important: EcoRI, although mentioned in the MCS, is not unique!
+* important: ''Eco''RI, although mentioned in the MCS, is not unique!
 <pubmed> 14717704 </pubmed>

@@ Line 18: / Line 18: @@
 **'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
 ** '''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
 <pubmed> 14717704 </pubmed>

@@ Line 3: / Line 3: @@
-* vector for the expression of proteins in E. coli, the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein
+* vector for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein
-* host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
+* host: ''E. coli'' BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
 * constructed in the [[Stülke]] lab
-* in E. coli: ampicillin resistance
+* in ''E. coli'': ampicillin resistance
 * based on pET3C

@@ Line 3: / Line 3: @@
-vector for the expression of proteins in E. coli, the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein
+* vector for the expression of proteins in E. coli, the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein
-host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
+* host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
-constructed in the [[Stülke]] lab
+* constructed in the [[Stülke]] lab
-in E. coli: ampicillin resistance
+* in E. coli: ampicillin resistance
-based on pET3C
+* based on pET3C
-similar [[plasmid]]: [[pGP574]]
+* similar [[plasmid]]: [[pGP574]]
-sequencing primer:
+* sequencing primer:
-*'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
+**'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
-*'''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
+** '''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
 <pubmed> 14717704 </pubmed>

@@ Line 4: / Line 4: @@
 '''Applications:'''
-Vector pGP172 was designed for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein. The ''E. coli'' strain BL21(DE3)/pLysS serves as a host strain and the expression of the desired protein is induced by the addition of 1 mM IPTG to the culture. pGP172 confers resistance to ampicillin in ''E. coli''. The plasmid was constructed in the [[Stülke]] lab and it is based on the vector pET3C.  pGP172 is similar to [[plasmid]] [[pGP574]].
+Vector pGP172 was designed for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein. The ''E. coli'' strain BL21(DE3)/pLysS serves as a host strain and the expression of the desired protein is induced by the addition of 1 mM IPTG to the culture. pGP172 confers resistance to ampicillin in ''E. coli''. The plasmid was constructed in the [[Stülke]] lab and it is based on the vector pET3C. pGP172 is similar [[plasmid]] to [[pGP574]].
 Sequencing primers:

← Older revision		Revision as of 18:37, 7 September 2009
Line 19:		Line 19:
	*'''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’		*'''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’

−		+	<pubmed> 14717704 </pubmed>
−	Merzbacher, M., Detsch, C., Hillen, W. & Stülke, J. (2004) ''Mycoplasma pneumoniae'' HPr kinase/ phosphorylase: Assigning functional roles to the P-loop and HPr kinase/ phosphorylase signature sequence motif. Eur. J. Biochem. 271: 367-374. [http://www.ncbi.nlm.nih.gov/sites/entrez/~~14717704 PubMed]~~