PGP1460 - Revision history

Jstuelk at 10:11, 28 May 2013

2013-05-28T10:11:51Z

Kgunka at 13:46, 2 May 2013

2013-05-02T13:46:30Z

Jstuelk at 14:27, 16 August 2012

2012-08-16T14:27:37Z

Fmehne at 12:29, 16 May 2012

2012-05-16T12:29:03Z

Fmehne at 12:26, 16 May 2012

2012-05-16T12:26:15Z

Jstuelk at 07:37, 9 May 2012

2012-05-09T07:37:06Z

Jstuelk at 16:13, 28 September 2010

2010-09-28T16:13:51Z

Lflorez at 13:25, 28 September 2010

2010-09-28T13:25:31Z

Lflorez at 13:25, 28 September 2010

2010-09-28T13:25:00Z

Heilers: Created page with "The vector was constructed in the Stülke lab and it is suitable for constitutive expression of C-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers res..."

2010-09-28T11:18:41Z

Created page with "The vector was constructed in the Stülke lab and it is suitable for constitutive expression of C-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers res..."

New page

The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive expression of C-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI. Just like pGP382 it can be used for the [[SPINE]] method. Detailed information about the multiple cloning site can be found here: [[pGP382]]. Gene cloned into this vector require a Shine-Dalgarno sequence.

Sequencing primers:
*'''M13_puc_for:''' 5‘-GTAAAACGACGGCCAGTG-3‘
*'''M13_puc_rev:''' 5‘-GGAAACAGCTATGACCATG-3‘

<pubmed> </pubmed>

← Older revision		Revision as of 10:11, 28 May 2013
Line 15:		Line 15:
	Similar plasmid: [[pGP1459]] (for N-terminal Strep-tags)		Similar plasmid: [[pGP1459]] (for N-terminal Strep-tags)

−	<pubmed> </pubmed>	+	<pubmed> 23192352 </pubmed>
		+
		+	== Back to [[Plasmids]] ==

@@ Line 2: / Line 2: @@
 [[File:PGP382_cloning_region.jpeg|250px|thumb|right|'''pGP382 cloning region: click to enlarge''']]
-The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive expression of C-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI. Just like [[pGP382]] it can be used for the [[SPINE]] method.
+The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive expression of C-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI. The plasmid will integrate into the ''[[ganA]]'' gene. Just like [[pGP382]] it can be used for the [[SPINE]] method.
 The cloning region of [[pGP382]] is shown for detailed information.

@@ Line 2: / Line 2: @@
 [[File:PGP382_cloning_region.jpeg|250px|thumb|right|'''pGP382 cloning region: click to enlarge''']]
 The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive expression of C-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI. Just like [[pGP382]] it can be used for the [[SPINE]] method.
 '''Genes cloned into this vector require a Shine-Dalgarno sequence.'''

← Older revision		Revision as of 12:26, 16 May 2012
Line 1:		Line 1:
	[[File:PGP1460.png\|250px\|thumb\|right\|'''pGP1460: click to enlarge''']]		[[File:PGP1460.png\|250px\|thumb\|right\|'''pGP1460: click to enlarge''']]
		+	[[File:PGP382_cloning_region.jpeg\|250px\|thumb\|right\|'''pGP382 cloning region: click to enlarge''']]

	The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive expression of C-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI. Just like [[pGP382]] it can be used for the [[SPINE]] method.		The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive expression of C-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI. Just like [[pGP382]] it can be used for the [[SPINE]] method.

← Older revision		Revision as of 07:37, 9 May 2012
Line 11:		Line 11:
	*'''M13_puc_rev:''' 5‘-GGAAACAGCTATGACCATG-3‘		*'''M13_puc_rev:''' 5‘-GGAAACAGCTATGACCATG-3‘

		+	Similar plasmid: [[pGP1459]] (for N-terminal Strep-tags)

	<pubmed> </pubmed>		<pubmed> </pubmed>

@@ Line 10: / Line 10: @@
 Sequencing primers:
-*'''M13_puc_for:''' 5‘-GTAAAACGACGGCCAGTG-3‘
+*'''KG64:''' 5‘-TATCAGGGCCTCGACTACA-3‘
-*'''M13_puc_rev:''' 5‘-GGAAACAGCTATGACCATG-3‘
+*'''ML107:''' 5‘-GCTTCATAGAGTAATTCTGTAAAGG-3‘
 Similar plasmid: [[pGP1459]] (for N-terminal Strep-tags)
 <pubmed>  </pubmed>

@@ Line 1: / Line 1: @@
 [[File:PGP1460.png|250px|thumb|right|'''pGP1460: click to enlarge''']]
-The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive expression of C-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI. Just like pGP382 it can be used for the [[SPINE]] method. Detailed information about the multiple cloning site can be found here: [[pGP382]]. Gene cloned into this vector require a Shine-Dalgarno sequence.
+The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive expression of C-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI. Just like [[pGP382]] it can be used for the [[SPINE]] method.
 Sequencing primers:

← Older revision		Revision as of 13:25, 28 September 2010
Line 1:		Line 1:
−	[[File:PGP1460.~~jpeg~~\|250px\|thumb\|right\|'''pGP1460: click to enlarge''']]	+	[[File:PGP1460.png\|250px\|thumb\|right\|'''pGP1460: click to enlarge''']]

	The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive expression of C-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI. Just like pGP382 it can be used for the [[SPINE]] method. Detailed information about the multiple cloning site can be found here: [[pGP382]]. Gene cloned into this vector require a Shine-Dalgarno sequence.		The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive expression of C-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI. Just like pGP382 it can be used for the [[SPINE]] method. Detailed information about the multiple cloning site can be found here: [[pGP382]]. Gene cloned into this vector require a Shine-Dalgarno sequence.