PGP1389 - Revision history

Jstuelk at 14:03, 3 June 2013

2013-06-03T14:03:16Z

Fmehne at 11:33, 30 October 2012

2012-10-30T11:33:49Z

Jstuelk at 12:54, 16 August 2011

2011-08-16T12:54:59Z

Fmehne at 12:45, 24 March 2011

2011-03-24T12:45:29Z

Jstuelk: moved Pgp1389 to PGP1389

2010-10-18T19:06:10Z

moved Pgp1389 to PGP1389

Jstuelk at 19:05, 18 October 2010

2010-10-18T19:05:32Z

MLehnik at 06:41, 18 October 2010

2010-10-18T06:41:51Z

MLehnik: Created page with "File:PGP1389 Subtiwiki.png '''General comments''' Integrative plasmid for the fusion of Streptag tag to the C-terminus of a protein, keeping the control of the expression u..."

2010-10-18T06:40:38Z

Created page with "File:PGP1389 Subtiwiki.png '''General comments''' Integrative plasmid for the fusion of Streptag tag to the C-terminus of a protein, keeping the control of the expression u..."

New page

[[File:PGP1389 Subtiwiki.png]]

'''General comments'''

Integrative plasmid for the fusion of Streptag tag to the C-terminus of a protein, keeping the control of the expression under its natural promotor
* ''E.coli'': multicopy plasmid, select for ampicillin
* ''B. subtilis'': no ori, select for spectinomycin

'''Hints for the usage'''
* amplify the last 600bp of your gene of interest,
* skip the Stopp-Codon of the gene in the reverse primer
* take care that the integration is in-frame

← Older revision		Revision as of 14:03, 3 June 2013
Line 15:		Line 15:
	'''The reference'''		'''The reference'''
	<pubmed>21803996</pubmed>		<pubmed>21803996</pubmed>
		+	== Return to [[Plasmids]] ==

@@ Line 3: / Line 3: @@
 '''General comments'''
-Integrative [[plasmid]] for the fusion of the C-terminus of a protein to a Streptag tag, keeping the control of expression under control of the natural promotor of the target gene.
+Integrative [[plasmid]] for the fusion of the C-terminus of a protein to a Strep tag, keeping the control of expression under control of the natural promotor of the target gene.
 * ''E.coli'': multicopy plasmid, select for ampicillin

← Older revision		Revision as of 12:54, 16 August 2011
Line 12:		Line 12:
	* skip the Stopp-Codon of the gene in the reverse primer		* skip the Stopp-Codon of the gene in the reverse primer
	* take care that the integration is in-frame		* take care that the integration is in-frame
		+
		+	'''The reference'''
		+	<pubmed>21803996</pubmed>

← Older revision		Revision as of 12:45, 24 March 2011
Line 1:		Line 1:
−	[[File:PGP1389 Subtiwiki.png\|250px\|thumb\|right\|'''~~pGP1331~~: click to enlarge''']]	+	[[File:PGP1389 Subtiwiki.png\|250px\|thumb\|right\|'''pGP1389: click to enlarge''']]

	'''General comments'''		'''General comments'''

← Older revision		Revision as of 06:41, 18 October 2010
Line 1:		Line 1:
−	[[File:PGP1389 Subtiwiki.png]]	+	[[File:PGP1389 Subtiwiki.png\|250px\|thumb\|right\|'''pGP1331: click to enlarge''']]

	'''General comments'''		'''General comments'''

← Older revision	Revision as of 19:06, 18 October 2010
(No difference)

@@ Line 3: / Line 3: @@
 '''General comments'''
-Integrative plasmid for the fusion of Streptag tag to the C-terminus of a protein, keeping the control of the expression under its natural promotor
+Integrative [[plasmid]] for the fusion of the C-terminus of a protein to a Streptag tag, keeping the control of expression under control of the natural promotor of the target gene.
 * ''E.coli'': multicopy plasmid, select for ampicillin
 * ''B. subtilis'': no ori, select for spectinomycin
-'''Hints for the usage'''
+'''Hints for the utilisation of the plasmid'''
 * amplify the last 600bp of your gene of interest,
 * skip the Stopp-Codon of the gene in the reverse primer
 * take care that the integration is in-frame