MutL - Revision history

Jstuelk: /* Original publications */

2014-10-27T13:41:20Z

‎Original publications

134.76.38.147 at 13:47, 2 April 2014

2014-04-02T13:47:27Z

134.76.70.252 at 08:28, 12 November 2013

2013-11-12T08:28:45Z

Jstuelk: /* References */

2013-09-13T17:24:21Z

‎References

Jstuelk at 08:48, 4 September 2013

2013-09-04T08:48:52Z

Jstuelk at 16:01, 25 July 2013

2013-07-25T16:01:14Z

Jstuelk at 13:36, 27 May 2013

2013-05-27T13:36:50Z

Jstuelk: /* References */

2013-05-27T13:35:46Z

‎References

134.76.70.252 at 13:25, 16 May 2013

2013-05-16T13:25:58Z

134.76.70.252 at 10:17, 14 May 2013

2013-05-14T10:17:56Z

← Older revision		Revision as of 13:41, 27 October 2014
Line 1:		Line 1:
−	* '''Description:~~''' DNA mismatch repair <br/><br/>~~	+	{{#bank: MutL}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''mutL''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| DNA mismatch repair~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| DNA repair~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17050 mutL]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MutL MutL]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 70 kDa, 5.583~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1881 bp, 627 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[mutS]]'', ''[[ymzD]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU17050 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU17050 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU17050 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:mutL_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mutL_1778337_1780220_1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:mutL_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17050]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[DNA repair/ recombination]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU17050
−
−	~~===Phenotypes of a mutant ===~~
−	* 60-fold increased mutation rate {{PubMed\|23882084}}
−	* a ''[[rnhB]] [[mutS]]-[[mutL]]'' mutant is not viable {{PubMed\|23882084}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU17050&redirect=T BSU17050]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mutSL.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11402]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−
−	* '''Protein family:''' DNA mismatch repair mutL/hexB family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[MutL]] (C-terminal domain)-[[DnaN]] {{PubMed\|21050827}}
−	** [[DnaE]]-[[MutL]] {{PubMed\|21958350}}
−	** [[MutL]]-[[MutS]] {{PubMed\|23998896}}
−
−	* '''[[Localization]]:'''
−	** forms foci at midcell position, the frequency of foci increases upon mismatch formation {{PubMed\|21958350}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU17050&redirect=T BSU17050]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3KDK 3KDK] (C-terminal domain) {{PubMed\|20603082}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P49850 P49850]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU17050]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[mutS]]-[[mutL]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/8760914 PubMed]
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mutL_1778337_1780220_1 mutL] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** A [[ncRNA]] is predicted between ''[[mutL]]'' and ''[[ymzD]]'' {{PubMed\|20525796}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** GP1190 (del ''[[mutS]]-[[mutL]]''::''aphA3'') {{PubMed\|22178973}}, available in [[Jörg Stülke]]'s lab
−	** 1A833 ( ''mutL''::''spec''), {{PubMed\|11779496}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A833&Search=1A833 BGSC]
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed> 22933559 </pubmed>~~
−	~~== Original publications ==~~
−	~~<pubmed>8760914, 15375129, 20603082 20525796 22843852 21050827,21958350 22178973 23882084 23998896 </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 56: / Line 56: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mutSL.html]
@@ Line 94: / Line 95: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3KDK 3KDK] (C-terminal domain) {{PubMed|20603082}}

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17050 mutL]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/MutL MutL]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MutL MutL]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 70 kDa, 5.583

@@ Line 141: / Line 141: @@
 =References=
 <pubmed>8760914, 15375129, 20603082 20525796 22843852 21050827,21958350 22178973 23882084 23998896 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 87: / Line 87: @@
 * '''[[SubtInteract|Interactions]]:'''
 ** [[MutL]] (C-terminal domain)-[[DnaN]] {{PubMed|21050827}}
-** [[DnaE]]-[[MutL]]  {{PubMed|21958350}}
+** [[DnaE]]-[[MutL]] {{PubMed|21958350}}
 * '''[[Localization]]:'''
@@ Line 140: / Line 141: @@
 =References=
-<pubmed>8760914, 15375129, 20603082 20525796 22843852 21050827,21958350 22178973 23882084</pubmed>
+<pubmed>8760914, 15375129, 20603082 20525796 22843852 21050827,21958350 22178973 23882084 23998896 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 52: / Line 52: @@
 ===Phenotypes of a mutant ===
 === Database entries ===
@@ Line 119: / Line 121: @@
 =Biological materials =
-* '''Mutant:''' GP1190 (del ''mutSL''::''aphA3'') available in the [[Stülke]] lab
+* '''Mutant:'''
 ** 1A833 ( ''mutL''::''spec''), {{PubMed|11779496}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A833&Search=1A833 BGSC]
@@ Line 137: / Line 140: @@
 =References=
-<pubmed>8760914, 15375129, 20603082 20525796 22843852 21050827,21958350</pubmed>
+<pubmed>8760914, 15375129, 20603082 20525796 22843852 21050827,21958350 22178973 23882084</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 37: / Line 37: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/>
@@ Line 112: / Line 108: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mutL_1778337_1780220_1 mutL] {{PubMed|22383849}}
-* '''Sigma factor:'''
+* '''[[Sigma factor]]:'''
 * '''Regulation:'''

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mutL_1778337_1780220_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:mutL_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mutL_1778337_1780220_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:mutL_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17050]]
 |-
 |}

@@ Line 24: / Line 24: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mutS]]'', ''[[ymzD]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU17050 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU17050 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU17050 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU17050 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU17050 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU17050 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:mutL_context.gif]]