MurAA - Revision history

134.76.70.252 at 09:55, 17 April 2014

2014-04-17T09:55:50Z

134.76.38.147 at 15:02, 2 April 2014

2014-04-02T15:02:02Z

Jkampf: /* Biological materials */

2014-03-14T09:26:43Z

‎Biological materials

134.76.70.252 at 11:52, 7 January 2014

2014-01-07T11:52:06Z

Jstuelk: /* References */

2013-09-09T16:51:45Z

‎References

Jstuelk: /* Biological materials */

2013-07-22T08:53:00Z

‎Biological materials

134.76.70.252 at 14:48, 16 May 2013

2013-05-16T14:48:16Z

134.76.70.252 at 11:30, 14 May 2013

2013-05-14T11:30:01Z

134.76.70.252 at 14:04, 13 May 2013

2013-05-13T14:04:39Z

Jstuelk at 16:01, 28 August 2012

2012-08-28T16:01:13Z

← Older revision		Revision as of 09:55, 17 April 2014
Line 1:		Line 1:
−	* '''Description:~~''' UDP-N-acetylglucosamine 1-carboxyvinyltransferase <br/><br/>~~	+	{{#bank: MurAA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''murAA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''murA ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| UDP-N-acetylglucosamine 1-carboxyvinyltransferase~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| peptidoglycan precursor biosynthesis~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36760 murAA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=murAA murAA]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 46 kDa, 5.45~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1308 bp, 436 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[spoIID]]'', ''[[ywmB]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36760 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36760 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36760 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:murAA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=murAA_3777949_3779259_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:murAA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36760]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/><br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[cell wall synthesis]]}},~~
−	~~{{SubtiWiki category\|[[biosynthesis of cell wall components]]}},~~
−	~~{{SubtiWiki category\|[[essential genes]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU36760
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU36760&redirect=T BSU36760]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/murAA.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11955]
−
−	~~=== Additional information===~~
−
−
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine (according to Swiss-Prot)
−
−	* '''Protein family:''' MurA subfamily (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):''' [[MurAB]]
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU36760&redirect=T BSU36760]
−
−	* '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=3SG1 3SG1] (from ''B. anthracis'', 79% identity, 94% similarity)
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P70965 P70965]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU36760]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/2.5.1.7 2.5.1.7]
−
−	~~=== Additional information===~~
−
−	~~:* [[MurAA]] is subject to [[ClpC]]-[[ClpP]]-dependent degradation upon glucose starvation {{PubMed\|14763982,17981983}}~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[murAA]]'' {{PubMed\|9353933}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=murAA_3777949_3779259_-1 murAA] {{PubMed\|22383849}}
−
−	* '''Sigma factor:'''
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:''' subject to Clp-dependent proteolysis upon glucose starvation {{PubMed\|17981983}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−	** pRSETA-murAA, Expression of His(6)-MurAA, avalable in [[Ulf Gerth]]'s lab {{PubMed\|14763982}}
−	**pGP2597: (IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]]), available in [[Jörg Stülke]]'s lab
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:''' avalable in [[Ulf Gerth]]'s lab {{PubMed\|14763982}}
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~== Reviews ==~~
−	~~<pubmed> 21822642 </pubmed>~~
−	~~== Original publications ==~~
−	~~<pubmed>14763982,18063720, 19899805,14763982, 8994972 9485407 </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 58: / Line 58: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/murAA.html]
@@ Line 95: / Line 96: @@
 === Database entries ===
 * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=3SG1 3SG1] (from ''B. anthracis'', 79% identity, 94% similarity)

@@ Line 128: / Line 128: @@
 * '''Expression vector:'''
 ** pRSETA-murAA, Expression of His(6)-MurAA, avalable in [[Ulf Gerth]]'s lab {{PubMed|14763982}}
 * '''lacZ fusion:'''

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36760 murAA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/cellwall.html Cell wall]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=murAA murAA]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 46 kDa, 5.45

@@ Line 142: / Line 142: @@
 =References=
+== Reviews ==
-<pubmed>14763982,18063720,,14763982, </pubmed>
+<pubmed> 21822642 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 127: / Line 127: @@
 * '''Expression vector:'''
 * '''lacZ fusion:'''
@@ Line 134: / Line 135: @@
 * '''two-hybrid system:'''
-* '''Antibody:'''
+* '''Antibody:''' avalable in [[Ulf Gerth]]'s lab {{PubMed|14763982}}
 =Labs working on this gene/protein=

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=murAA_3777949_3779259_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:murAA_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=murAA_3777949_3779259_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:murAA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36760]]
 |-
 |}

@@ Line 24: / Line 24: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spoIID]]'', ''[[ywmB]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36760 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36760 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36760 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36760 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36760 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36760 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:murAA_context.gif]]

@@ Line 37: / Line 37: @@
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