MscL - Revision history

134.76.38.147 at 15:00, 2 April 2014

2014-04-02T15:00:13Z

Jstuelk: /* Reviews */

2014-03-06T12:25:39Z

‎Reviews

134.76.70.252 at 11:50, 7 January 2014

2014-01-07T11:50:41Z

134.76.70.252 at 14:46, 16 May 2013

2013-05-16T14:46:52Z

134.76.70.252 at 11:28, 14 May 2013

2013-05-14T11:28:26Z

134.76.70.252 at 14:03, 13 May 2013

2013-05-13T14:03:02Z

134.76.70.252 at 12:21, 19 September 2012

2012-09-19T12:21:21Z

Jstuelk at 07:58, 29 August 2012

2012-08-29T07:58:03Z

134.76.70.252 at 16:46, 7 August 2012

2012-08-07T16:46:45Z

134.76.70.252 at 10:51, 23 April 2012

2012-04-23T10:51:53Z

← Older revision		Revision as of 15:00, 2 April 2014
Line 1:		Line 1:
−	* '''Description:~~''' large conductance [[transport proteins\|mechanosensitive channel]] protein, prevents selective release of cytoplasmic proteins in a hypotonic environment <br/><br/>~~	+	{{#bank: MscL}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''mscL''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''ywpC ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| large conductance [[transport proteins\|mechanosensitive channel]] protein~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| resistance to osmotic downshockglycine betaine export~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36360 mscL]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=mscL mscL]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 14 kDa, 4.649~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 390 bp, 130 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[ywpD]]'', ''[[ywpB]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36360 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36360 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36360 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:mscL_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mscL_3743267_3743659_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:mscL_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36360]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/><br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[coping with hypo-osmotic stress]]}},~~
−	~~{{SubtiWiki category\|[[membrane proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU36360
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~sensitive to osmotic downshock (> 0.5 M) during logarithmic growth [http://www.ncbi.nlm.nih.gov/sites/entrez/19252899 PubMed]~~
−
−	~~=== Database entries ===~~
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mscL.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12497]
−
−	~~=== Additional information===~~
−
−
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−
−	* '''Protein family:''' mscL family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:''' cell membrane [http://www.ncbi.nlm.nih.gov/sites/entrez/19252899 PubMed]
−
−	~~=== Database entries ===~~
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=3HZQ 3HZQ] (MscL of ''Staphylococcus aureus'') {{PubMed\|19701184}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P94585 P94585]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU36360]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''mscL'' {{PubMed\|9353933}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mscL_3743267_3743659_-1 mscL] {{PubMed\|22383849}}
−
−	* '''Sigma factor:'''
−
−	* '''Regulation:''' expressed in logarithmic phase [http://www.ncbi.nlm.nih.gov/sites/entrez/19252899 PubMed]
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** 1A957 ( ''mscL''::''spec''), {{PubMed\|18310427}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A957&Search=1A957 BGSC]
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~[[Jan Maarten van Dijl]], Groningen, Netherlands~~
−
−	~~[[Erhard Bremer]], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed>12626684 ,19727188 , 20825352 22685280 24258154 </pubmed>~~
−
−	~~==Original Publications==~~
−	~~<pubmed>17665170,18310427,19160392,16897034,19252899, 19701184 9632260 19738010 9353933 </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 144: / Line 144: @@
 =References=
 ==Reviews==
-<pubmed>12626684 ,19727188 , 20825352 22685280 </pubmed>
+<pubmed>12626684 ,19727188 , 20825352 22685280 24258154 </pubmed>
 ==Original Publications==

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36360 mscL]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=mscL mscL]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 4.649

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mscL_3743267_3743659_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:mscL_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mscL_3743267_3743659_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:mscL_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36360]]
 |-
 |}

@@ Line 24: / Line 24: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywpD]]'', ''[[ywpB]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36360 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36360 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36360 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36360 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36360 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36360 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:mscL_context.gif]]

← Older revision		Revision as of 12:21, 19 September 2012
Line 122:		Line 122:

	* '''Mutant:'''		* '''Mutant:'''
		+	** 1A957 ( ''mscL''::''spec''), {{PubMed\|18310427}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A957&Search=1A957 BGSC]

	* '''Expression vector:'''		* '''Expression vector:'''

@@ Line 37: / Line 37: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/><br/><br/><br/><br/>
@@ Line 147: / Line 143: @@
 =References=
 ==Reviews==
-<pubmed>12626684 ,19727188 , 20825352 </pubmed>
+<pubmed>12626684 ,19727188 , 20825352 22685280 </pubmed>
 ==Original Publications==

@@ Line 13: / Line 13: @@
 |-
 |style="background:#ABCDEF;" align="center"|'''Function''' || resistance to osmotic downshockglycine betaine export
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''