MotB - Revision history

Jstuelk: /* References */

2014-10-04T15:24:46Z

‎References

Jstuelk: /* Basic information/ Evolution */

2014-05-05T08:18:36Z

‎Basic information/ Evolution

134.76.38.147 at 13:33, 2 April 2014

2014-04-02T13:33:05Z

Jstuelk at 12:34, 16 February 2014

2014-02-16T12:34:05Z

Jstuelk at 12:30, 16 February 2014

2014-02-16T12:30:01Z

Jstuelk: /* Phenotypes of a mutant */

2013-12-04T20:17:43Z

‎Phenotypes of a mutant

Jstuelk: /* Phenotypes of a mutant */

2013-12-04T19:40:39Z

‎Phenotypes of a mutant

Jstuelk at 19:39, 4 December 2013

2013-12-04T19:39:39Z

134.76.70.252 at 16:07, 11 November 2013

2013-11-11T16:07:06Z

134.76.70.252 at 13:07, 16 May 2013

2013-05-16T13:07:05Z

← Older revision		Revision as of 15:24, 4 October 2014
Line 1:		Line 1:
−	* '''Description:~~''' H+-coupled [[MotA]]-[[~~MotB~~]] flagellar stator <br/><br/>~~	+	{{#bank: MotB}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''motB''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''mot ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| motility protein (flagellar motor rotation)~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| [[motility and chemotaxis]]~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13680 motB]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MotB MotB]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 29 kDa, 6.652~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 783 bp, 261 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[mhqR]]'', ''[[motA]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU13680 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU13680 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU13680 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:motB_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=motB_1433676_1434461_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:motB_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13680]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[motility and chemotaxis]]}},~~
−	~~{{SubtiWiki category\|[[membrane proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[SigD regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU13680
−
−	~~===Phenotypes of a mutant ===~~
−	* loss of swimming and swarming motility {{PubMed\|24296669}}
−	* mucoid phenotype due to the [[DegU]]-P activated overexpression of the ''[[capB]]-[[capC]]-[[capA]]-[[capE]]'' operon and resulting overproduction of poly-gamma-glutamate {{PubMed\|24296669,23888912}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU13680&redirect=T BSU13680]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/motAB.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10689]
−
−	~~=== Additional information===~~
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−
−	* '''Protein family:''' OmpA-like domain (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):''' [[MotS]]
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−	** protonation of Asp-24 is essential for torque generation {{PubMed\|23888912,9573160}}
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:''' [[MotA]]-[[MotB]]
−
−	* '''Localization:''' cell membrane (according to Swiss-Prot)
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU13680&redirect=T BSU13680]
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P28612 P28612]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU13680]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[motA]]-[[motB]]'' {{PubMed\|6313226}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=motB_1433676_1434461_-1 motB] {{PubMed\|22383849}}
−
−	* '''Sigma factor:''' [[SigD]] {{PubMed\|6313226}}
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:''' available in [[Nicola Stanley-Wall]]'s lab {{PubMed\|23888912}}
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~<pubmed>6313226 24296669 23888912 9573160</pubmed>~~
−	~~[[Category:Protein-coding genes]]~~

@@ Line 74: / Line 74: @@
 * '''Protein family:''' OmpA-like domain (according to Swiss-Prot)
-* '''Paralogous protein(s):'''
+* '''Paralogous protein(s):''' [[MotS]]
 === Extended information on the protein ===

@@ Line 57: / Line 57: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/motAB.html]
@@ Line 93: / Line 94: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 82: / Line 82: @@
 * '''Modification:'''
 * '''[[Cofactors]]:'''
@@ Line 136: / Line 137: @@
 =References=
-<pubmed>6313226 24296669 23888912</pubmed>
+<pubmed>6313226 24296669 23888912 9573160</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 54: / Line 54: @@
 ===Phenotypes of a mutant ===
 * loss of swimming and swarming motility {{PubMed|24296669}}
-* mucoid phenotype due to the [[DegU]]-P activated overexpression of the ''[[capB]]-[[capC]]-[[capA]]-[[capE]]'' operon and resulting overproduction of poly-gamma-glutamate {{PubMed|24296669}}
+* mucoid phenotype due to the [[DegU]]-P activated overexpression of the ''[[capB]]-[[capC]]-[[capA]]-[[capE]]'' operon and resulting overproduction of poly-gamma-glutamate {{PubMed|24296669,23888912}}
 === Database entries ===
@@ Line 119: / Line 119: @@
 =Biological materials =
-* '''Mutant:'''
+* '''Mutant:''' available in [[Nicola Stanley-Wall]]'s lab {{PubMed|23888912}}
 * '''Expression vector:'''
@@ Line 136: / Line 136: @@
 =References=
-<pubmed>6313226 24296669</pubmed>
+<pubmed>6313226 24296669 23888912</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 53: / Line 53: @@
 ===Phenotypes of a mutant ===
-** loss of swimming and swarming motility {{PubMed|24296669}}
+* loss of swimming and swarming motility {{PubMed|24296669}}
-** mucoid phenotype due to the [[DegU]]-P activated overexpression of the ''[[pgsB]]-[[pgsC]]-[[pgsA]]'' operon and resulting overproduction of poly-gamma-glutamate {{PubMed|24296669}}
+* mucoid phenotype due to the [[DegU]]-P activated overexpression of the ''[[pgsB]]-[[pgsC]]-[[pgsA]]'' operon and resulting overproduction of poly-gamma-glutamate {{PubMed|24296669}}
 === Database entries ===

@@ Line 37: / Line 37: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/>
@@ Line 57: / Line 53: @@
 ===Phenotypes of a mutant ===
 === Database entries ===
@@ Line 81: / Line 79: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 138: / Line 136: @@
 =References=
-<pubmed>6313226 </pubmed>
+<pubmed>6313226 24296669</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13680 motB]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/MotB MotB]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MotB MotB]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 6.652

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=motB_1433676_1434461_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:motB_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=motB_1433676_1434461_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:motB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13680]]
 |-
 |}