MntA - Revision history

134.76.70.252 at 14:10, 17 April 2014

2014-04-17T14:10:21Z

134.76.70.252 at 10:00, 17 April 2014

2014-04-17T10:00:13Z

134.76.38.147 at 14:35, 2 April 2014

2014-04-02T14:35:26Z

Jstuelk: /* Phenotypes of a mutant */

2014-02-10T15:18:29Z

‎Phenotypes of a mutant

Jstuelk at 15:17, 10 February 2014

2014-02-10T15:17:49Z

134.76.70.252 at 11:31, 7 January 2014

2014-01-07T11:31:36Z

134.76.70.252 at 09:12, 12 November 2013

2013-11-12T09:12:08Z

134.76.70.252 at 14:23, 16 May 2013

2013-05-16T14:23:32Z

134.76.70.252 at 11:05, 14 May 2013

2013-05-14T11:05:40Z

134.76.70.252 at 13:41, 13 May 2013

2013-05-13T13:41:12Z

← Older revision		Revision as of 14:10, 17 April 2014
Line 1:		Line 1:
−	* '''Description:~~''' manganese [[ABC transporter]] (binding protein, lipoprotein) <br/><br/>~~	+	{{#bank: MntA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''mntA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''ytgA ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| manganese [[ABC transporter]] (binding protein)~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| manganese uptake~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU30770 mntA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MntA MntA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=mntA mntA]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 33 kDa, 6.162~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 918 bp, 306 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[mntB]]'', ''[[menC]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU30770 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU30770 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU30770 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:mntA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mntA_3145038_3145958_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:mntA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU30770]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[ABC transporters]]}},~~
−	~~{{SubtiWiki category\|[[trace metal homeostasis (Cu, Zn, Ni, Mn, Mo)]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[MntR regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU30770
−
−	~~===Phenotypes of a mutant ===~~
−	* essential according to [http://www.ncbi.nlm.nih.gov/pubmed/17114254 Kobayashi et al.], but a mutant has been constructed and studied by [http://www.ncbi.nlm.nih.gov/pubmed/10760146 Que and Helmann, 2000]
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU30770&redirect=T BSU30770]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mntABCD.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13851]
−
−	~~=== Additional information===~~
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−
−	* '''Protein family:''' bacterial solute-binding protein 9 family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):''' [[ZnuA]]
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[MntB]]-([[MntC]]-[[MntD]])-[[MntA]] {{PubMed\|10092453}}
−
−	* '''[[Localization]]:''' cell membrane (according to Swiss-Prot), extracellular lipoprotein (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU30770&redirect=T BSU30770]
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/O34385 O34385]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU30770]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−	* '''Operon:''' ''[[mntA]]-[[mntB]]-[[mntC]]-[[mntD]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/10760146 PubMed]
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mntA_3145038_3145958_-1 mntA] {{PubMed\|22383849}}
−
−	* '''Sigma factor:'''
−
−	* '''Regulation:'''
−	** repressed at high Mn(II) concentrations ([[MntR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12950915 PubMed]
−
−	* '''Regulatory mechanism:'''
−	** [[MntR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12950915 PubMed]
−
−	* '''Additional information:'''
−	** A [[ncRNA]] is predicted between ''[[mntA]]'' and ''[[menC]]'' {{PubMed\|20525796}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 96 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~[[John Helmann]], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−
−	~~<pubmed>10760146,10092453,,12950915,10760146,18957862 10760146 17114254,20525796 </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 122: / Line 122: @@
 * '''Additional information:'''
 ** A [[ncRNA]] is predicted between ''[[mntA]]'' and ''[[menC]]'' {{PubMed|20525796}}
 =Biological materials =

@@ Line 58: / Line 58: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mntABCD.html]
@@ Line 94: / Line 95: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 55: / Line 55: @@
 ===Phenotypes of a mutant ===
-* essential according to [http://www.ncbi.nlm.nih.gov/pubmed/17114254 Kobayashi et al.], but a mutant has been constructed and studied by [http://www.ncbi.nlm.nih.gov/pubmed/10760146 Que amd Helmann, 2000]
+* essential according to [http://www.ncbi.nlm.nih.gov/pubmed/17114254 Kobayashi et al.], but a mutant has been constructed and studied by [http://www.ncbi.nlm.nih.gov/pubmed/10760146 Que and Helmann, 2000]
 === Database entries ===

@@ Line 8: / Line 8: @@
 |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ytgA ''
 |-
-|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/17114254 PubMed]
+|style="background:#ABCDEF;" align="center"| '''Essential''' || no
 |-
 |style="background:#ABCDEF;" align="center"| '''Product''' || manganese [[ABC transporter]] (binding protein)
@@ Line 43: / Line 43: @@
 = [[Categories]] containing this gene/protein =
 {{SubtiWiki category|[[ABC transporters]]}},
-{{SubtiWiki category|[[trace metal homeostasis (Cu, Zn, Ni, Mn, Mo)]]}},
+{{SubtiWiki category|[[trace metal homeostasis (Cu, Zn, Ni, Mn, Mo)]]}}
 = This gene is a member of the following [[regulons]] =
@@ Line 56: / Line 55: @@
 ===Phenotypes of a mutant ===
+* essential according to [http://www.ncbi.nlm.nih.gov/pubmed/17114254 Kobayashi et al.], but a mutant has been constructed and studied by [http://www.ncbi.nlm.nih.gov/pubmed/10760146 Que amd Helmann, 2000]
 === Database entries ===

@@ Line 18: / Line 18: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MntA MntA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=mntA mntA]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 6.162

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU30770 mntA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/MntA MntA]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MntA MntA]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''

@@ Line 31: / Line 31: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mntA_3145038_3145958_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:mntA_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mntA_3145038_3145958_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:mntA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU30770]]
 |-
 |}

@@ Line 26: / Line 26: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mntB]]'', ''[[menC]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU30770 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU30770 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU30770 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU30770 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU30770 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU30770 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:mntA_context.gif]]