Mbl - Revision history

134.76.70.252 at 14:09, 17 April 2014

2014-04-17T14:09:49Z

134.76.70.252 at 09:54, 17 April 2014

2014-04-17T09:54:31Z

134.76.38.147 at 15:00, 2 April 2014

2014-04-02T15:00:27Z

Jstuelk at 19:41, 14 December 2013

2013-12-14T19:41:15Z

134.76.70.252 at 09:31, 12 November 2013

2013-11-12T09:31:20Z

Jstuelk at 08:20, 30 July 2013

2013-07-30T08:20:09Z

134.76.70.252 at 14:47, 16 May 2013

2013-05-16T14:47:07Z

134.76.70.252 at 11:28, 14 May 2013

2013-05-14T11:28:40Z

134.76.70.252 at 14:03, 13 May 2013

2013-05-13T14:03:16Z

Fmehne: /* Extended information on the protein */

2012-09-27T05:47:13Z

‎Extended information on the protein

← Older revision		Revision as of 14:09, 17 April 2014
Line 1:		Line 1:
−	* '''Description:~~''' [[cell shape]]-determining protein, forms filaments, the polymers control/restrict the mobility of the cell wall elongation enzyme complex, required for [[CwlO]] activity <br/><br/>~~	+	{{#bank: Mbl}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''mbl''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| [[MreB]]-like protein~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| [[cell shape]] determination~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36410 mbl]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=Mbl Mbl]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 35 kDa, 5.669~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 999 bp, 333 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[flhO]]'', ''[[spoIIID]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36410 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36410 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36410 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:mbl_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mbl_3747254_3748255_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:mbl_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36410]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[cell shape]]}},~~
−	~~{{SubtiWiki category\|[[sporulation proteins]]}},~~
−	~~{{SubtiWiki category\|[[membrane proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[SigE regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[stringent response]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU36410
−
−	~~===Phenotypes of a mutant ===~~
−	* non-viable in the presence of low Mg(2+)
−	* readily accumulate ''[[rsgI]]'' suppressor mutants [http://www.ncbi.nlm.nih.gov/sites/entrez/19114499 PubMed]
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU36410&redirect=T BSU36410]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/usd-spoIIID-mbl-flhOP.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10916]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−	** forms helical filaments in a heterologous system {{PubMed\|21091501}}
−	** required for [[CwlO]] activity {{PubMed\|23869552}}
−
−	* '''Protein family:''' ftsA/mreB family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):''' [[MreB]], [[MreBH]]
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** part of the [[cell wall biosynthetic complex]] {{PubMed\|21636744,21636745}}
−	** [[MreB]]-[[Mbl]] {{PubMed\|21636744}}
−	** [[Mbl]]-[[MreBH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17064365 PubMed]
−	** [[RodZ]]-[[Mbl]] {{PubMed\|23879732}}
−
−	* '''[[Localization]]:'''
−	** during logarithmic growth, [[Mbl]] forms discrete patches that move processively along peripheral tracks perpendicular to the cell axis {{PubMed\|21636744}}
−	** forms transverse bands as cells enter the stationary phase {{PubMed\|21636744}}
−	** close to the inner surface of the cytoplasmic membrane [http://www.ncbi.nlm.nih.gov/sites/entrez/16950129 PubMed]
−	** reports on helical structures formed by Mbl {{PubMed\|16950129,20566861}} seem to be misinterpretation of data {{PubMed\|21636744}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU36410&redirect=T BSU36410]
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P39751 P39751]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU36410]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−	* '''Operon:''' ''[[usd]]-[[spoIIID]]-[[mbl]]-[[flhO]]-[[flhP]]'' {{PubMed\|9023218,7836311}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mbl_3747254_3748255_-1 mbl] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigE]] {{PubMed\|9023218}}
−
−	* '''Regulation:'''
−	** expressed early during sporulation in the mother cell ([[SigE]]) {{PubMed\|9023218}}
−	** [[RelA]] dependent downregulation (Class I) during stringent response {{PubMed\|11948165}}
−	** expression is strongly repressed in the presence of aminoguanidine (inhibitor of nitric oxide synthase) {{PubMed\|22048160}}
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** An [[ncRNA\|antisense RNA]] is predicted for ''[[mbl]]'' {{PubMed\|20525796}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 336 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 2526 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Boris Görke\| Görke]] lab
−
−	* '''Antibody:''' available in the [[Jeff Errington]] and [[Peter Graumann]] labs
−
−	~~=Labs working on this gene/protein=~~
−
−	~~[[Peter Graumann]], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Localization==~~
−	~~<pubmed> 17064365, 20566861 21636744,21636745 22069484</pubmed>~~
−	~~==Other original publications==~~
−	~~<pubmed>19643765,16832063,11290328,12809607,16101995,12530960,7836311,,16950129 9023218, 19659933 19114499, 21091501 20525796 22048160 22343529 23879732 23869552</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 132: / Line 132: @@
 * '''Additional information:'''
 ** An [[ncRNA|antisense RNA]] is predicted for ''[[mbl]]'' {{PubMed|20525796}}
 =Biological materials =

@@ Line 59: / Line 59: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/usd-spoIIID-mbl-flhOP.html]
@@ Line 103: / Line 104: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 1: / Line 1: @@
-* '''Description:''' [[cell shape]]-determining protein, forms filaments, the polymers control/restrict the mobility of the cell wall elongation enzyme complex <br/><br/>
+* '''Description:''' [[cell shape]]-determining protein, forms filaments, the polymers control/restrict the mobility of the cell wall elongation enzyme complex, required for [[CwlO]] activity <br/><br/>
 {| align="right" border="1" cellpadding="2"
@@ Line 55: / Line 55: @@
 ===Phenotypes of a mutant ===
+* non-viable in the presence of low Mg(2+)
-non-viable in the presence of low Mg(2+), readily accumulate ''[[rsgI]]'' suppressor mutants [http://www.ncbi.nlm.nih.gov/sites/entrez/19114499 PubMed]
+* readily accumulate ''[[rsgI]]'' suppressor mutants [http://www.ncbi.nlm.nih.gov/sites/entrez/19114499 PubMed]
 === Database entries ===
@@ Line 72: / Line 72: @@
 * '''Catalyzed reaction/ biological activity:'''
 ** forms helical filaments in a heterologous system {{PubMed|21091501}}
 * '''Protein family:''' ftsA/mreB family (according to Swiss-Prot)
@@ Line 81: / Line 82: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 154: / Line 155: @@
 <pubmed> 17064365,  20566861 21636744,21636745 22069484</pubmed>
 ==Other original publications==
-<pubmed>19643765,16832063,11290328,12809607,16101995,12530960,7836311,,16950129 9023218, 19659933 19114499, 21091501 20525796 22048160 22343529 23879732</pubmed>
+<pubmed>19643765,16832063,11290328,12809607,16101995,12530960,7836311,,16950129 9023218, 19659933 19114499, 21091501 20525796 22048160 22343529 23879732 23869552</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36410 mbl]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/Mbl Mbl]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=Mbl Mbl]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 5.669

@@ Line 37: / Line 37: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/>
@@ Line 97: / Line 93: @@
 ** [[MreB]]-[[Mbl]] {{PubMed|21636744}}
 ** [[Mbl]]-[[MreBH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17064365 PubMed]
 * '''[[Localization]]:'''
@@ Line 121: / Line 118: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mbl_3747254_3748255_-1 mbl] {{PubMed|22383849}}
-* '''Sigma factor:''' [[SigE]] {{PubMed|9023218}}
+* '''[[Sigma factor]]:''' [[SigE]] {{PubMed|9023218}}
 * '''Regulation:'''
@@ Line 157: / Line 154: @@
 <pubmed> 17064365,  20566861 21636744,21636745 22069484</pubmed>
 ==Other original publications==
-<pubmed>19643765,16832063,11290328,12809607,16101995,12530960,7836311,,16950129 9023218, 19659933 19114499, 21091501 20525796 22048160 22343529</pubmed>
+<pubmed>19643765,16832063,11290328,12809607,16101995,12530960,7836311,,16950129 9023218, 19659933 19114499, 21091501 20525796 22048160 22343529 23879732</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mbl_3747254_3748255_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:mbl_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mbl_3747254_3748255_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:mbl_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36410]]
 |-
 |}

@@ Line 24: / Line 24: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[flhO]]'', ''[[spoIIID]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36410 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36410 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36410 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36410 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36410 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36410 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:mbl_context.gif]]

@@ Line 99: / Line 99: @@
 * '''[[Localization]]:'''
-** during logarithmic growth, [[Mbl]] forms discrete patches thst move processively along peripheral tracks perpendicular to the cell axis {{PubMed|21636744}}
+** during logarithmic growth, [[Mbl]] forms discrete patches that move processively along peripheral tracks perpendicular to the cell axis {{PubMed|21636744}}
 ** forms transverse bands as cells enter the stationary phase {{PubMed|21636744}}
 ** close to the inner surface of the cytoplasmic membrane [http://www.ncbi.nlm.nih.gov/sites/entrez/16950129 PubMed]