LytE - Revision history

Jstuelk at 18:58, 1 February 2015

2015-02-01T18:58:58Z

Show changes

Jstuelk: /* Biological materials */

2014-09-24T13:41:45Z

‎Biological materials

134.76.38.147 at 13:17, 2 April 2014

2014-04-02T13:17:39Z

Jstuelk: /* Original publications */

2013-12-28T10:27:58Z

‎Original publications

Jstuelk: /* Extended information on the protein */

2013-12-27T14:49:16Z

‎Extended information on the protein

Jstuelk at 14:46, 27 December 2013

2013-12-27T14:46:02Z

Jstuelk at 10:28, 23 December 2013

2013-12-23T10:28:56Z

Jstuelk at 19:35, 14 December 2013

2013-12-14T19:35:00Z

Jstuelk: /* Extended information on the protein */

2013-09-17T13:47:36Z

‎Extended information on the protein

Jstuelk at 17:21, 2 August 2013

2013-08-02T17:21:46Z

← Older revision		Revision as of 13:41, 24 September 2014
Line 152:		Line 152:

	* '''two-hybrid system:'''		* '''two-hybrid system:'''
		+
		+	* '''FLAG-tag construct:'''
		+	** GP2020 ''lytE-3xFLAG spec'' (based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab

	* '''Antibody:'''		* '''Antibody:'''

@@ Line 63: / Line 63: @@
 * cells are thinner (reduced diameter) as compared to the wild type {{PubMed|23869552}}
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/lytE.html]
@@ Line 107: / Line 108: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 161: / Line 161: @@
 <pubmed>18430080 23066944</pubmed>
 == Original publications ==
-<pubmed>21261835,22211522 16950129,17581128,14594841,9457885,9573210,10322020, 24125693 20059685,14651647, 23869552 21541672 22139507 23855774</pubmed>
+<pubmed>21261835,22211522 16950129,17581128,14594841,9457885,9573210,10322020, 24125693 23199363 20059685,14651647, 23869552 21541672 22139507 23855774</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 86: / Line 86: @@
 * '''[[Domains]]:'''
-** contains four N-acetylglucosamine-polymer-binding [[LysM domain]]s {{PubMed|18430080}}
+** contains three N-acetylglucosamine-polymer-binding [[LysM domain]]s {{PubMed|18430080}}
 ** C-terminal D,L-endopeptidase domain {{PubMed|22139507}}

@@ Line 86: / Line 86: @@
 * '''[[Domains]]:'''
 ** C-terminal D,L-endopeptidase domain {{PubMed|22139507}}
@@ Line 157: / Line 158: @@
 =References=
-== Reviews ==
+== Reviews==
-<pubmed>23066944</pubmed>
+<pubmed>18430080 23066944</pubmed>
 == Original publications ==
 <pubmed>21261835,22211522 16950129,17581128,14594841,9457885,9573210,10322020, 24125693 20059685,14651647, 23869552 21541672 22139507 23855774</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 127: / Line 127: @@
 * '''Regulation:'''
 ** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
-** induced at high temperature ([[SigI]]) {{PubMed|21541672}}
+** induced at high temperature ([[SigI]], [[WalR]]) {{PubMed|24125693,21541672}}
 * '''Regulatory mechanism:'''
-** [[WalR]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/17581128 PubMed]
+** [[WalR]]: transcription activation {{PubMed|24125693,17581128}}
 ** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
@@ Line 159: / Line 160: @@
 <pubmed>23066944</pubmed>
 == Original publications ==
-<pubmed>21261835,22211522 16950129,17581128,14594841,9457885,9573210,10322020, 20059685,14651647,23869552 21541672 22139507 23855774</pubmed>
+<pubmed>21261835,22211522 16950129,17581128,14594841,9457885,9573210,10322020, 24125693 20059685,14651647, 23869552 21541672 22139507 23855774</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 59: / Line 59: @@
 * growth defect at high temperature {{PubMed|21541672}}
 * inactivation of ''[[lytE]]'' strongly restores beta-lactam resistance in a ''[[sigM]]'' mutant by delaying cell lysis {{PubMed|22211522}}
-* a ''[[lytE]]'' mutation is synthetically lethal with  ''[[ftsE]]'' and ''[[ftsX]]'' mutation (due to a lack of autolysin activity) {{PubMed|23855774}}
+* a ''[[lytE]]'' mutation is synthetically lethal with  ''[[ftsE]]'' and ''[[ftsX]]'' mutation (due to a lack of autolysin activity) {{PubMed|23869552,23855774}}
 * a ''[[lytE]]'' mutation increases the cell separation defect of a ''[[lytF]]'' mutant {{PubMed|23855774}}
+* cells are thinner (reduced diameter) as compared to the wild type {{PubMed|23869552}}
 === Database entries ===
@@ Line 85: / Line 85: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 ** C-terminal D,L-endopeptidase domain {{PubMed|22139507}}
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
-** activity requires a functional [[MreBH]] {{PubMed|16950129}}
+** activity requires functional [[MreB]] and [[MreBH]] {{PubMed|23869552,16950129}}
 * '''[[SubtInteract|Interactions]]:'''
@@ Line 101: / Line 101: @@
 * '''[[Localization]]:'''
 ** binds the cell wall {{PubMed|21261835}}
 ** localizes to cell septa, poles and lateral sidewall of the cell (via the N-terminal domain) {{PubMed|22139507}}
 === Database entries ===
@@ Line 159: / Line 159: @@
 <pubmed>23066944</pubmed>
 == Original publications ==
-<pubmed>21261835,22211522 16950129,17581128,14594841,9457885,9573210,10322020, 20059685,14651647, 21541672 22139507 23855774</pubmed>
+<pubmed>21261835,22211522 16950129,17581128,14594841,9457885,9573210,10322020, 20059685,14651647,23869552 21541672 22139507 23855774</pubmed>
 [[Category:Protein-coding genes]]

← Older revision		Revision as of 13:47, 17 September 2013
Line 93:		Line 93:

	* '''Effectors of protein activity:'''		* '''Effectors of protein activity:'''
		+	** activity requires a functional [[MreBH]] {{PubMed\|16950129}}

	* '''[[SubtInteract\|Interactions]]:'''		* '''[[SubtInteract\|Interactions]]:'''

@@ Line 1: / Line 1: @@
-* '''Description:''' cell wall hydrolase (major autolysin) for cell elongation, D,L-endopeptidase-type autolysin <br/><br/>
+* '''Description:''' cell wall hydrolase (major autolysin) for cell elongation and separation, D,L-endopeptidase-type autolysin <br/><br/>
 {| align="right" border="1" cellpadding="2"
 |-
@@ Line 11: / Line 11: @@
 |style="background:#ABCDEF;" align="center"| '''Product''' || cell wall hydrolase (major autolysin),endopeptidase-type autolysin
 |-
-|style="background:#ABCDEF;" align="center"|'''Function''' || major autolysin, cell elongation
+|style="background:#ABCDEF;" align="center"|'''Function''' || major autolysin, cell elongation and separation
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU09420 lytE]