List of plasmids - Revision history

CB: /* Expression/ purification in E. coli */

2019-06-26T09:14:10Z

‎Expression/ purification in E. coli

CB: /* Expression/ purification in B. subtilis */

2019-06-26T09:10:19Z

‎Expression/ purification in B. subtilis

CB: /* Expression/ purification in B. subtilis */

2019-06-26T08:44:28Z

‎Expression/ purification in B. subtilis

CB: /* Expression/ purification in B. subtilis */

2019-06-26T08:44:02Z

‎Expression/ purification in B. subtilis

Bzhu: /* Expression/ purification in B. subtilis */

2017-08-30T10:57:41Z

‎Expression/ purification in B. subtilis

Bzhu at 10:35, 30 August 2017

2017-08-30T10:35:55Z

Bzhu at 08:30, 30 August 2017

2017-08-30T08:30:20Z

Bzhu at 08:29, 30 August 2017

2017-08-30T08:29:58Z

Bzhu at 11:27, 28 August 2017

2017-08-28T11:27:16Z

Jstuelk at 11:59, 13 July 2016

2016-07-13T11:59:56Z

@@ Line 63: / Line 63: @@
 *[[pGP172]]: expression of proteins in ''E. coli'' allows fusion to a Strep-tag at the N-terminus of the protein
 *[[pGP574]]: expression of proteins in ''E. coli'' allows fusion to a Strep-tag at the C-terminus of the protein
 == Construction of (markerless) deletions ==

@@ Line 33: / Line 33: @@
 * [[pGP1389]]: integrative plasmid for the fusion of a Strep-tag to the C-terminus of a protein, keeping expression under the control of the natural promoter
 *[[pGP3273]]: integrative expression vector for ''B. subtilis'' (integrates in ''[[amyE]]''); allowing the expression of genes under the control of the IPTG-inducible Pgrac promoter, ''E.coli / B. subtilis'' shuttle vector (amp/cm)
 <div style="border: 1px dashed #aaa; margin: 5px -5px; padding: 0 5px">
 <b>P''grac'' vectors</b>

@@ Line 32: / Line 32: @@
 *[[pGP1460]]: integration vector (integrates in ''[[ganA]]'') analogous to [[pGP382]]; expression of proteins in ''B. subtilis'' allows fusion to a Strep-tag at the C-terminus of the protein
 * [[pGP1389]]: integrative plasmid for the fusion of a Strep-tag to the C-terminus of a protein, keeping expression under the control of the natural promoter
-*[[pGP3273]]: integrative expression vector for ''B. subtilis''(integrates in ''[[amyE]]''); allowing the expression of genes under the control of the IPTG-inducible Pgrac promoter, ''E.coli / B. subtilis'' shuttle vector (amp/cm)
+*[[pGP3273]]: integrative expression vector for ''B. subtilis'' (integrates in ''[[amyE]]''); allowing the expression of genes under the control of the IPTG-inducible Pgrac promoter, ''E.coli / B. subtilis'' shuttle vector (amp/cm)
 <div style="border: 1px dashed #aaa; margin: 5px -5px; padding: 0 5px">
 <b>P''grac'' vectors</b>

@@ Line 32: / Line 32: @@
 *[[pGP1460]]: integration vector (integrates in ''[[ganA]]'') analogous to [[pGP382]]; expression of proteins in ''B. subtilis'' allows fusion to a Strep-tag at the C-terminus of the protein
 * [[pGP1389]]: integrative plasmid for the fusion of a Strep-tag to the C-terminus of a protein, keeping expression under the control of the natural promoter
 <div style="border: 1px dashed #aaa; margin: 5px -5px; padding: 0 5px">
 <b>P''grac'' vectors</b>

@@ Line 32: / Line 32: @@
 *[[pGP1460]]: integration vector (integrates in ''[[ganA]]'') analogous to [[pGP382]]; expression of proteins in ''B. subtilis'' allows fusion to a Strep-tag at the C-terminus of the protein
 * [[pGP1389]]: integrative plasmid for the fusion of a Strep-tag to the C-terminus of a protein, keeping expression under the control of the natural promoter
+<div style="border: 1px dashed #aaa; margin: 5px -5px; padding: 0 5px">
 <b>P''grac'' vectors</b>
 * [[pHT01]]: Expression vector for high level protein production with ''B. subtilis''. Expression of the gene of interest will be under control of the IPTG inducible Pgrac promoter. ''E.coli / B. subtilis'' shuttle vector (amp/cm)
@@ Line 52: / Line 50: @@
 * [[pTTB1]]: Low copy food grade expression vector; constitutive expression; selection is based on the interplay of the endogenous ''Bacillus'' toxin EndoA (encoded within the genome of host strains ''B. subtilis'' TEA or ''B. subtilis'' WEA) and its antitoxin EndoB (vector encoded); ''B. subtilis / E.coli'' shuttle vector
 * [[pTTB2]]: High copy food grade expression vector; constitutive expression; selection is based on the interplay of the endogenous ''Bacillus'' toxin EndoA (encoded within the genome of host strains ''B. subtilis'' TEA or ''B. subtilis'' WEA) and its antitoxin EndoB (vector encoded); ''B. subtilis / E.coli'' shuttle vector
+<p style="float:right; font-weight:bold; color: navy; padding:5px">Content from [https://www.mobitec.com/cms/index.html MoBiTec GmbH] [[File:Mobiteclogo.png|40px]] </p>
 </div>

@@ Line 37: / Line 37: @@
 <b>Sponsored content</b>
 <b>P''grac'' vectors</b>
@@ Line 56: / Line 58: @@
 * [[pTTB2]]: High copy food grade expression vector; constitutive expression; selection is based on the interplay of the endogenous ''Bacillus'' toxin EndoA (encoded within the genome of host strains ''B. subtilis'' TEA or ''B. subtilis'' WEA) and its antitoxin EndoB (vector encoded); ''B. subtilis / E.coli'' shuttle vector
 </div>

@@ Line 43: / Line 43: @@
 *[[pMAD]]: construction of markerless KOs in Gram-positive bacteria
-== A collection of biobricks for the work with ''B. subtilis'' ==
+== Toolboxes for the work with ''B. subtilis'' ==
 * the biobrick box {{PubMed|24295448}}