KtrB - Revision history

Jstuelk: /* References */

2015-04-17T16:06:30Z

‎References

134.76.38.147 at 14:36, 2 April 2014

2014-04-02T14:36:35Z

Jstuelk: /* Expression and regulation */

2014-02-19T15:52:28Z

‎Expression and regulation

134.76.70.252 at 11:32, 7 January 2014

2014-01-07T11:32:39Z

Jstuelk at 17:08, 14 December 2013

2013-12-14T17:08:53Z

134.76.70.252 at 14:20, 18 November 2013

2013-11-18T14:20:07Z

Jstuelk: /* Biological materials */

2013-08-01T14:01:17Z

‎Biological materials

Jstuelk: /* Biological materials */

2013-07-03T11:43:21Z

‎Biological materials

Jstuelk: /* References */

2013-06-13T12:14:21Z

‎References

134.76.70.252 at 14:24, 16 May 2013

2013-05-16T14:24:51Z

← Older revision		Revision as of 16:06, 17 April 2015
Line 1:		Line 1:
−	* '''Description:~~''' high affinity potassium transporter [[KtrA]]-[[~~KtrB~~]], integral membrane subunit <br/><br/>~~	+	{{#bank: KtrB}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''ktrB''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''yubG''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| high affinity potassium transporter [[KtrA]]-[[KtrB]], <br/>integral membrane subunit (proton symport)~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| potassium uptake~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31100 ktrB]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=KtrB KtrB]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://cellpublisher.gobics.de/view/GqbblRJkBH Metal ion homeostasis], [http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=ktrB ktrB]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 48 kDa, 10.116~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1335 bp, 445 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[ktrA]]'', ''[[yubF]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31100 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31100 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31100 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:yubG_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ktrB_3189089_3190426_1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:ktrB_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31100]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[transporters/ other]]}},~~
−	~~{{SubtiWiki category\|[[metal ion homeostasis (K, Na, Ca, Mg)]]}},~~
−	~~{{SubtiWiki category\|[[coping with hyper-osmotic stress]]}},~~
−	~~{{SubtiWiki category\|[[membrane proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[YdaO riboswitch]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU31100
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU31100&redirect=T BSU31100]
−
−	* '''DBTBS entry:''' no entry
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13956]
−
−	~~=== Additional information===~~
−
−
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−
−	* '''Protein family:'''
−
−	* '''Paralogous protein(s):''' [[KtrD]]
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** KtrB forms dimers {{PubMed\|17932047}}
−	** [[KtrA]]-[[KtrB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12562800 PubMed], composed of an octameric [[KtrA]] ring and two [[KtrB]] dimers {{PubMed\|23598340}}
−
−	* '''[[Localization]]:''' integral membrane protein [http://www.ncbi.nlm.nih.gov/sites/entrez/12562800 PubMed]
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU31100&redirect=T BSU31100]
−
−	* '''Structure:'''
−	** [http://www.pdb.org/pdb/explore/explore.do?structureId=4J7C 4J7C] (the [[KtrA]]-[[KtrB]] complex) {{PubMed\|23598340}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/O32081 O32081]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU31100]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[ktrA]]-[[ktrB]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/12562800 PubMed]
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ktrB_3189089_3190426_1 ktrB] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−
−	* '''Regulation:'''
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−	** expression is controlled via termination antitermination by the [[ydaO riboswitch]], expression is switched off upon binding od c-di-AMP {{PubMed\|24141192,20511502}}
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** 1A954 ( ''ktrB''::''kan''), {{PubMed\|12562800}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A954&Search=1A954 BGSC]
−	** GHB1 (D(''[[ktrA]]-[[ktrB]]'')::''aphA3''), available in [[Erhard Bremer]]'s lab
−	** GP92 (D(''[[ktrA]]-[[ktrB]]'')::''aphA3''), available in [[Jörg Stülke]]'s lab
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~[[Erhard Bremer]], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−
−	~~<pubmed>12562800,20511502, 17932047 23086297 23598340 16990138 24141192</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 59: / Line 59: @@
 === Database entries ===
 * '''DBTBS entry:''' no entry
@@ Line 98: / Line 99: @@
 === Database entries ===
 * '''Structure:'''

← Older revision		Revision as of 15:52, 19 February 2014
Line 125:		Line 125:

	* '''Additional information:'''		* '''Additional information:'''
−

	=Biological materials =		=Biological materials =

@@ Line 18: / Line 18: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=KtrB KtrB]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://cellpublisher.gobics.de/view/GqbblRJkBH Metal ion homeostasis], [http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://cellpublisher.gobics.de/view/GqbblRJkBH Metal ion homeostasis], [http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=ktrB ktrB]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 48 kDa, 10.116

@@ Line 83: / Line 83: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 119: / Line 119: @@
 * '''Regulation:'''
-** induced if the energy charge is low ([[ydaO riboswitch]])  {{PubMed|23086297}}
+* '''Regulation:'''
 * '''Regulatory mechanism:'''
-** expression is controlled via termination antitermination by the [[ydaO riboswitch]] {{PubMed|23086297,20511502}}
+** expression is controlled via termination antitermination by the [[ydaO riboswitch]], expression is switched off upon binding od c-di-AMP {{PubMed|24141192,20511502}}
 * '''Additional information:'''
 =Biological materials =
@@ Line 151: / Line 152: @@
 =References=
-<pubmed>12562800,20511502, 17932047 23086297 23598340 16990138 </pubmed>
+<pubmed>12562800,20511502, 17932047 23086297 23598340 16990138 24141192</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31100 ktrB]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/KtrB KtrB]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=KtrB KtrB]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://cellpublisher.gobics.de/view/GqbblRJkBH Metal ion homeostasis], [http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''

@@ Line 131: / Line 131: @@
 ** 1A954 ( ''ktrB''::''kan''), {{PubMed|12562800}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A954&Search=1A954 BGSC]
 ** GHB1 (D(''[[ktrA]]-[[ktrB]]'')::''aphA3''), available in [[Erhard Bremer]]'s lab
 * '''Expression vector:'''

@@ Line 31: / Line 31: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ktrB_3189089_3190426_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:ktrB_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ktrB_3189089_3190426_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:ktrB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31100]]
 |-
 |}