HlpB - Revision history

134.76.38.147 at 13:22, 2 April 2014

2014-04-02T13:22:17Z

134.76.70.252 at 12:56, 16 May 2013

2013-05-16T12:56:13Z

134.76.70.252 at 09:52, 14 May 2013

2013-05-14T09:52:15Z

134.76.70.252 at 12:30, 13 May 2013

2013-05-13T12:30:25Z

Jstuelk at 15:35, 21 September 2012

2012-09-21T15:35:06Z

Jstuelk at 15:29, 21 September 2012

2012-09-21T15:29:52Z

Jstuelk: /* Categories containing this gene/protein */

2012-09-21T14:36:54Z

‎Categories containing this gene/protein

Jstuelk at 14:34, 21 September 2012

2012-09-21T14:34:18Z

Jstuelk at 14:20, 21 September 2012

2012-09-21T14:20:06Z

Jstuelk at 13:06, 21 September 2012

2012-09-21T13:06:37Z

← Older revision		Revision as of 13:22, 2 April 2014
Line 1:		Line 1:
−	* '''Description:''' HNH nuclease-like protein, rescues [[AddA]]-[[AddB]]-mediated recombination intermediates <br/><br/>	+	{{#bank: HlpB}}
−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''hlpB''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''yisB ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| yes~~ {{~~PubMed\|22984257}}~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| HNH nuclease-like protein~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| rescuing of [[AddA]]-[[AddB]]-mediated recombination intermediates~~
−	\|-
−	~~\|colspan="2" style="background:~~#~~FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]'''~~: ~~[http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU10660 yisB]~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 11 kDa, 9.941~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 300 bp, 100 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[sbcC]]'', ''[[gerPF]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU10660 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU10660 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU10660 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:yisB_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	~~\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yisB_1148155_1148457_1 Expression at a glance]'''   {{PubMed\|22383849}~~}~~<br/>[[Image:yisB_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU10660]]~~
−	\|-
−	\|}

−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[DNA repair/ recombination]]}},~~
−	~~{{SubtiWiki category\|[[essential genes]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[ComK regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU10660
−
−	~~===Phenotypes of a mutant ===~~
−	* essential, the mutation can be suppressed by second-site mutations in ''[[addA]]'' or ''[[addB]]'' {{PubMed\|22984257}}
−	~~=== Database entries ===~~
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sbcD-yirY-yisB.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13089]
−
−	~~=== Additional information===~~
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−
−	* '''Protein family:'''
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:'''
−	** evenly distributed throughout the cytosol in growing cells {{PubMed\|22984257}}
−	** forms a single focus on the nucleoid upon induction of DNA damage {{PubMed\|22984257}}
−
−	~~=== Database entries ===~~
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/O06715 O06715]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU10660]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[addB]]-[[addA]]-[[sbcD]]-[[sbcC]]-[[hlpB]]'' {{PubMed\|22383849}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yisB_1148155_1148457_1 yisB] {{PubMed\|22383849}}
−
−	* '''Sigma factor:'''
−
−	* '''Regulation:'''
−	** positive regulation by [[ComK]] {{PubMed\|11948146}}
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~<pubmed> 11948146 22984257 22383849</pubmed>~~
−	~~[[Category:Protein-coding genes]]~~

@@ Line 26: / Line 26: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yisB_1148155_1148457_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:yisB_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yisB_1148155_1148457_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:yisB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU10660]]
 |-
 |}

@@ Line 21: / Line 21: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sbcC]]'', ''[[gerPF]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU10660 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU10660 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU10660 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU10660 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU10660 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU10660 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:yisB_context.gif]]

@@ Line 13: / Line 13: @@
 |style="background:#ABCDEF;" align="center"|'''Function''' || rescuing of [[AddA]]-[[AddB]]-mediated recombination intermediates
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU10660 yisB]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU10660 yisB]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 11 kDa, 9.941
@@ Line 21: / Line 21: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sbcC]]'', ''[[gerPF]]''
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12906&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU10660 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU10660 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU10660 Advanced_DNA]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:yisB_context.gif]]

@@ Line 85: / Line 85: @@
 * '''[[Localization]]:'''
+** evenly distributed throughout the cytosol in growing cells {{PubMed|22984257}}
 === Database entries ===

@@ Line 7: / Line 7: @@
 |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yisB ''
 |-
-|style="background:#ABCDEF;" align="center"| '''Essential''' || no
+|style="background:#ABCDEF;" align="center"| '''Essential''' || yes {{PubMed|22984257}}
 |-
 |style="background:#ABCDEF;" align="center"| '''Product''' || HNH nuclease-like protein
@@ Line 50: / Line 50: @@
 ===Phenotypes of a mutant ===
+* essential, the mutation can be suppressed by second-site mutations in ''[[addA]]'' or ''[[addB]]'' {{PubMed|22984257}}
 === Database entries ===

@@ Line 37: / Line 37: @@
 = [[Categories]] containing this gene/protein =
-{{SubtiWiki category|[[DNA repair/ recombination]]}}
+{{SubtiWiki category|[[DNA repair/ recombination]]}},
 = This gene is a member of the following [[regulons]] =

@@ Line 99: / Line 99: @@
 =Expression and regulation=
-* '''Operon:''' ''[[sbcD]]-[[sbcC]]-[[yisB]]'' (according to [http://dbtbs.hgc.jp/COG/prom/sbcD-yirY-yisB.html DBTBS])
+* '''Operon:''' ''[[addB]]-[[addA]]-[[sbcD]]-[[sbcC]]-[[hlpB]]'' {{PubMed|22383849}}
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yisB_1148155_1148457_1 yisB] {{PubMed|22383849}}
@@ Line 131: / Line 131: @@
 =References=
-<pubmed> 11948146 22984257 </pubmed>
+<pubmed> 11948146 22984257 22383849</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 1: / Line 1: @@
-* '''Description:''' HNH nuclease-like protein <br/><br/>
+* '''Description:''' HNH nuclease-like protein, rescues [[AddA]]-[[AddB]]-mediated recombination intermediates <br/><br/>
 {| align="right" border="1" cellpadding="2"
 |-
@@ Line 11: / Line 11: @@
 |style="background:#ABCDEF;" align="center"| '''Product''' || HNH nuclease-like protein
 |-
-|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
+|style="background:#ABCDEF;" align="center"|'''Function''' || rescuing of [[AddA]]-[[AddB]]-mediated recombination intermediates
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU10660 yisB]