GlnR - Revision history

Jstuelk: /* Original publications */

2015-07-30T09:15:28Z

‎Original publications

Jstuelk: /* Database entries */

2015-02-19T09:22:46Z

‎Database entries

Jstuelk at 09:22, 19 February 2015

2015-02-19T09:22:28Z

Jstuelk: /* Biological materials */

2014-09-24T08:11:12Z

‎Biological materials

Jstuelk: /* Biological materials */

2014-09-24T08:10:17Z

‎Biological materials

Jstuelk at 13:17, 22 June 2014

2014-06-22T13:17:46Z

134.76.38.147 at 13:48, 2 April 2014

2014-04-02T13:48:51Z

134.76.70.252 at 10:51, 7 January 2014

2014-01-07T10:51:58Z

134.76.70.252 at 08:29, 12 November 2013

2013-11-12T08:29:49Z

134.76.70.252 at 13:27, 16 May 2013

2013-05-16T13:27:22Z

← Older revision		Revision as of 09:15, 30 July 2015
Line 1:		Line 1:
−	* '''Description:~~''' transcriptional repressor ([[MerR family]]) of the'' [[glnR]]-[[glnA]]'' operon <br/><br/>~~	+	{{#bank: GlnR}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''glnR''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| transcription repressor ([[MerR family]])~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| regulation of glutamine synthesis~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17450 glnR]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=GlnR GlnR]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=glnR glnR]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 15 kDa, 9.731~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 405 bp, 135 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[ynbB]]'', ''[[glnA]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU17450 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU17450 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU17450 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:glnR_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glnR_1877959_1878366_1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:glnR_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17450]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[biosynthesis/ acquisition of amino acids]]}}, [[glutamate metabolism]],~~
−	~~{{SubtiWiki category\|[[transcription factors and their control]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[GlnR regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[TnrA regulon]]}}~~
−	~~=The [[GlnR regulon]]:=~~
−	* ''[[glnR]]-[[glnA]]'', ''[[tnrA]]'', ''[[ureA]]-[[ureB]]-[[ureC]]''
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU17450
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU17450&redirect=T BSU17450]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/glnRA.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10424]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−
−	* '''Protein family:''' [[MerR family]]
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):''' feedback-inhibited [[GlnA]] {{PubMed\|18195355}}
−
−	* '''Effectors of protein activity:''' activity is enhanced upon interaction of the C-terminal domain with [[GlnA]] {{PubMed\|18331450}}
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[GlnR]]-[[GlnA]], with feedback-inhibited [[GlnA]], this results in DNA binding {{PubMed\|18195355}}
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU17450&redirect=T BSU17450]
−
−	* '''Structure:'''
−	** [http://pdb.org/pdb/search/structidSearch.do?structureId=4r4e 4R4E] (the GlnR-DNA complex) {{PubMed\|25691471}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P37582 P37582]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU17450]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[glnR]]-[[glnA]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/8636055 PubMed]
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glnR_1877959_1878366_1 glnR] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigA]] {{PubMed\|2906311}}
−
−	* '''Regulation:'''
−	** expressed in the absence of glutamine ([[GlnR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8636055 PubMed]
−	** repressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8799114 PubMed]
−
−	* '''Regulatory mechanism:'''
−	** [[GlnR]]-[[GlnA]] complex: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/8636055 PubMed]
−	** [[TnrA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/8799114 PubMed]
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:''' BP148 (del(''[[glnR]]-[[glnA]]'')::''cat''), available in [[Fabian Commichau]]'s lab
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:''' pGP189 (in [[pAC7]]), available in [[Jörg Stülke]]'s lab
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~[[Susan Fisher]], Boston, USA [http://www.bumc.bu.edu/microbiology/research/susan-h-fisher-phd/ homepage]~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed>22625175 </pubmed>~~
−	~~==Original publications==~~
−	~~<pubmed>12374841,9287005,8799114, 18195355, 2573733, 8636055, 19233925 2906311 18331450 16547045 23535029 25691471</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 98: / Line 98: @@
 * '''Structure:'''
-** [[http://pdb.org/pdb/search/structidSearch.do?structureId=4r4e 4R4E]] (the GlnR-DNA complex) {{PubMed|25691471}}
+** [http://pdb.org/pdb/search/structidSearch.do?structureId=4r4e 4R4E] (the GlnR-DNA complex) {{PubMed|25691471}}
 * '''UniProt:''' [http://www.uniprot.org/uniprot/P37582 P37582]

@@ Line 98: / Line 98: @@
 * '''Structure:'''
 * '''UniProt:''' [http://www.uniprot.org/uniprot/P37582 P37582]
@@ Line 149: / Line 150: @@
 <pubmed>22625175 </pubmed>
 ==Original publications==
-<pubmed>12374841,9287005,8799114, 18195355, 2573733, 8636055, 19233925 2906311 18331450 16547045 23535029</pubmed>
+<pubmed>12374841,9287005,8799114, 18195355, 2573733, 8636055, 19233925 2906311 18331450 16547045 23535029 25691471</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 127: / Line 127: @@
 =Biological materials =
-* '''Mutant:''' BP148 (del(''[[glnR]]-[[glnA]]'')::''cat''), availablr in [[Fabian Commichau]]'s lab
+* '''Mutant:''' BP148 (del(''[[glnR]]-[[glnA]]'')::''cat''), available in [[Fabian Commichau]]'s lab
 * '''Expression vector:'''

@@ Line 127: / Line 127: @@
 =Biological materials =
-* '''Mutant:'''
+* '''Mutant:''' BP148 (del(''[[glnR]]-[[glnA]]'')::''cat''), availablr in [[Fabian Commichau]]'s lab
 * '''Expression vector:'''
-* '''lacZ fusion:''' pGP189 (in [[pAC7]]), available in [[Stülke]] lab
+* '''lacZ fusion:''' pGP189 (in [[pAC7]]), available in [[Jörg Stülke]]'s lab
 * '''GFP fusion:'''

@@ Line 1: / Line 1: @@
-* '''Description:''' transcriptional repressor of the'' [[glnR]]-[[glnA]]'' operon <br/><br/>
+* '''Description:''' transcriptional repressor ([[MerR family]]) of the'' [[glnR]]-[[glnA]]'' operon <br/><br/>
 {| align="right" border="1" cellpadding="2"
@@ Line 10: / Line 10: @@
 |style="background:#ABCDEF;" align="center"| '''Essential''' || no
 |-
-|style="background:#ABCDEF;" align="center"| '''Product''' || transcription repressor
+|style="background:#ABCDEF;" align="center"| '''Product''' || transcription repressor ([[MerR family]])
 |-
 |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of glutamine synthesis
@@ Line 66: / Line 66: @@
 === Additional information===
 =The protein=
@@ Line 76: / Line 73: @@
 * '''Catalyzed reaction/ biological activity:'''
-* '''Protein family:'''
+* '''Protein family:''' [[MerR family]]
 * '''Paralogous protein(s):'''

@@ Line 59: / Line 59: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/glnRA.html]
@@ Line 97: / Line 98: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 18: / Line 18: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=GlnR GlnR]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate], [http://subtiwiki.uni-goettingen.de/pathways/cellwall.html Cell wall], [http://subtiwiki.uni-goettingen.de/pathways/other_nitrogen_sources/index.html Alternative nitrogen sources]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=glnR glnR]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 15 kDa, 9.731

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17450 glnR]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/GlnR GlnR]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=GlnR GlnR]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate], [http://subtiwiki.uni-goettingen.de/pathways/cellwall.html Cell wall], [http://subtiwiki.uni-goettingen.de/pathways/other_nitrogen_sources/index.html Alternative nitrogen sources]'''

@@ Line 31: / Line 31: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glnR_1877959_1878366_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:glnR_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glnR_1877959_1878366_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:glnR_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17450]]
 |-
 |}