GlnA - Revision history

Jstuelk: /* References */

2015-07-30T09:15:45Z

‎References

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Jstuelk at 09:23, 19 February 2015

2015-02-19T09:23:28Z

Jstuelk at 08:18, 24 September 2014

2014-09-24T08:18:45Z

Jstuelk at 08:11, 24 September 2014

2014-09-24T08:11:03Z

134.76.70.252 at 14:07, 17 April 2014

2014-04-17T14:07:10Z

134.76.70.252 at 09:29, 17 April 2014

2014-04-17T09:29:48Z

134.76.38.147 at 13:48, 2 April 2014

2014-04-02T13:48:55Z

Jstuelk at 13:01, 5 March 2014

2014-03-05T13:01:08Z

134.76.70.252 at 10:52, 7 January 2014

2014-01-07T10:52:02Z

Jstuelk at 17:11, 13 November 2013

2013-11-13T17:11:35Z

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 ** [http://www.pdb.org/pdb/explore.do?structureId=4LNN 4LNN] (apo-GS) {{PubMed|24158439}}
 ** [http://www.rcsb.org/pdb/explore/explore.do?structureId=3QAJ 3QAJ] (complex with ATP)
 ** [http://www.rcsb.org/pdb/101/motm.do?momID=30 A general discussion of GS structure]
@@ Line 176: / Line 177: @@
 ==Original publications==
-<pubmed>19233925, 20389117,8799114,18195355, 11719184, 12139611, 2573733, 8636055, 19233925, 16493705, 16885465, 6141156 2906311 20656908 16055443 18331450 16547045 8093698 21435182 23535029 24158439 15378759</pubmed>
+<pubmed>19233925, 20389117,8799114,18195355, 11719184, 12139611, 2573733, 8636055, 19233925, 16493705, 16885465, 6141156 2906311 20656908 16055443 18331450 16547045 8093698 21435182 23535029 24158439 15378759 25691471</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 151: / Line 151: @@
 ** GP247 (''glnA::cat''), available in [[Jörg Stülke]]'s lab
 ** BP148 (del(''[[glnR]]-[[glnA]]'')::''cat''), available in [[Fabian Commichau]]'s lab
 * '''Expression vector:'''

@@ Line 148: / Line 148: @@
 =Biological materials =
-* '''Mutant:''' GP247 (''glnA::cat''), available in [[Stülke]] lab
+* '''Mutant:'''
 * '''Expression vector:'''
-** expression/ purification from ''E. coli'', with N-terminal Strep-tag (in [[pGP172]]): pGP174, available in [[Stülke]] lab
+** expression/ purification from ''E. coli'', with N-terminal Strep-tag (in [[pGP172]]): pGP174, available in [[Jörg Stülke]]'s lab
 ** pGP177 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pBQ200]]), available in [[Jörg Stülke]]'s lab
-* '''lacZ fusion:'''  ''[[glnR]]-lacZ'': pGP189 (in [[pAC7]]), available in [[Stülke]] lab
+* '''lacZ fusion:'''  ''[[glnR]]-lacZ'': pGP189 (in [[pAC7]]), available in [[Jörg Stülke]]'s lab
 * '''GFP fusion:'''
@@ Line 160: / Line 162: @@
 * '''two-hybrid system:'''
-* '''Antibody:''' available in [[Karl Forchhammer]] lab
+* '''Antibody:''' available in [[Karl Forchhammer]]'s lab
 =Labs working on this gene/protein=

@@ Line 143: / Line 143: @@
 ** number of protein molecules per cell (minimal medium with glucose and ammonium): 8140 {{PubMed|24696501}}
 ** number of protein molecules per cell (complex medium with amino acids, without glucose): 11334 {{PubMed|24696501}}
 =Biological materials =
 * '''Mutant:''' GP247 (''glnA::cat''), available in [[Stülke]] lab

@@ Line 141: / Line 141: @@
 * '''Additional information:'''
 ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
 =Biological materials =

@@ Line 63: / Line 63: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/glnRA.html]
@@ Line 105: / Line 106: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 45: / Line 45: @@
 {{SubtiWiki category|[[transcription factors and their control]]}},
 {{SubtiWiki category|[[trigger enzyme]]}},
-{{SubtiWiki category|[[phosphoproteins]]}}
+{{SubtiWiki category|[[phosphoproteins]]}},
 = This gene is a member of the following [[regulons]] =
@@ Line 83: / Line 84: @@
 * '''Kinetic information:'''	K(M) for: Glu: 27 mM, ATP: 2.4 mM, ammonium: 0.18 mM; v(max): 3.7 µmol/min/mg
-* '''Domains:''' glutamate binding flap (aa 300 ... 306: protects unstable intermediates from abberant hydrolysis)
+* '''[[Domains]]:''' glutamate binding flap (aa 300 ... 306: protects unstable intermediates from abberant hydrolysis)
 * '''Modification:'''
@@ Line 89: / Line 90: @@
 ** ''in vitro'' phosphorylated by [[PrkC]] on Thr-26, Thr-147, Ser-207, and Thr-286 {{PubMed|20389117}}
-* '''Cofactor(s):''' Mg(2+)
+* '''[[Cofactors]]:''' Mg(2+)
 * '''Effectors of protein activity:'''
@@ Line 137: / Line 138: @@
 * '''Additional information:'''
 =Biological materials =
@@ Line 165: / Line 167: @@
 ==Original publications==
-<pubmed>19233925, 20389117,8799114,18195355, 11719184, 12139611, 2573733, 8636055, 19233925, 16493705, 16885465, 6141156 2906311 20656908 16055443 18331450 16547045 8093698 21435182 23535029 24158439 </pubmed>
+<pubmed>19233925, 20389117,8799114,18195355, 11719184, 12139611, 2573733, 8636055, 19233925, 16493705, 16885465, 6141156 2906311 20656908 16055443 18331450 16547045 8093698 21435182 23535029 24158439 15378759</pubmed>
 [[Category:Protein-coding genes]]

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 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=GlnA GlnA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=glnA glnA]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 50 kDa, 4.874