GbsB - Revision history

134.76.38.147 at 14:36, 2 April 2014

2014-04-02T14:36:23Z

134.76.70.252 at 11:32, 7 January 2014

2014-01-07T11:32:27Z

Jstuelk at 07:55, 22 August 2013

2013-08-22T07:55:39Z

134.76.70.252 at 14:24, 16 May 2013

2013-05-16T14:24:40Z

134.76.70.252 at 11:06, 14 May 2013

2013-05-14T11:06:42Z

134.76.70.252 at 13:42, 13 May 2013

2013-05-13T13:42:11Z

134.76.70.252 at 12:07, 19 September 2012

2012-09-19T12:07:32Z

134.76.70.252 at 15:20, 7 August 2012

2012-08-07T15:20:03Z

134.76.70.252 at 11:48, 20 April 2012

2012-04-20T11:48:10Z

134.76.70.252 at 16:06, 16 April 2012

2012-04-16T16:06:19Z

← Older revision		Revision as of 14:36, 2 April 2014
Line 1:		Line 1:
−	* '''Description:~~''' choline dehydrogenase (FAD-dependent), glycine betaine synthesis <br/><br/>~~	+	{{#bank: GbsB}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''gbsB''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| choline dehydrogenase (FAD-dependent)~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| osmoprotection~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31050 gbsB]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=gbsB gbsB]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 43 kDa, 4.912~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1209 bp, 403 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[yuaD]]'', ''[[gbsA]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31050 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31050 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31050 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:gbsB_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gbsB_3183867_3185075_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:gbsB_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31050]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[miscellaneous metabolic pathways]]}},~~
−	~~{{SubtiWiki category\|[[coping with hyper-osmotic stress]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[GbsR regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU31050
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−
−	* '''DBTBS entry:''' no entry
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11941]
−
−	~~=== Additional information===~~
−
−
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' An alcohol + NAD<sup>+</sup> = an aldehyde or ketone + NADH (according to Swiss-Prot)
−
−	* '''Protein family:''' iron-containing alcohol dehydrogenase family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P71017 P71017]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU31050]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/1.1.1.1 1.1.1.1]
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−	* '''Operon:''' '' [[gbsA]]-[[gbsB]]'' {{PubMed\|8752328}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gbsB_3183867_3185075_-1 gbsB] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigA]] {{PubMed\|8752328}}
−
−	* '''Regulation:'''
−	** induced by choline ([[GbsR]]) {{PubMed\|22408163,8752328}}
−
−	* '''Regulatory mechanism:'''
−	** [[GbsR]]: transcription repression {{PubMed\|22408163}}
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** 1A1056 ( ''gbsB''::''kan''), {{PubMed\|21296969}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1056&Search=1A1056 BGSC]
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~[[Erhard Bremer]], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~<pubmed>,8752328, 21296969 22408163</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31050 gbsB]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=gbsB gbsB]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 4.912

@@ Line 37: / Line 37: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
-<br/><br/><br/><br/>
+<br/><br/>
 = [[Categories]] containing this gene/protein =
@@ Line 48: / Line 44: @@
 = This gene is a member of the following [[regulons]] =
+{{SubtiWiki regulon|[[GbsR regulon]]}}
 =The gene=
@@ Line 112: / Line 108: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gbsB_3183867_3185075_-1 gbsB] {{PubMed|22383849}}
-* '''Sigma factor:''' [[SigA]] {{PubMed|8752328}}
+* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|8752328}}
 * '''Regulation:'''
@@ Line 144: / Line 140: @@
 =References=
-'''Additional publications:''' {{PubMed|22408163}}
+<pubmed>,8752328, 21296969 22408163</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gbsB_3183867_3185075_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:gbsB_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gbsB_3183867_3185075_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:gbsB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31050]]
 |-
 |}

@@ Line 24: / Line 24: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yuaD]]'', ''[[gbsA]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31050 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31050 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31050 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31050 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31050 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31050 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:gbsB_context.gif]]

← Older revision		Revision as of 12:07, 19 September 2012
Line 125:		Line 125:

	* '''Mutant:'''		* '''Mutant:'''
		+	** 1A1056 ( ''gbsB''::''kan''), {{PubMed\|21296969}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1056&Search=1A1056 BGSC]

	* '''Expression vector:'''		* '''Expression vector:'''

@@ Line 13: / Line 13: @@
 |-
 |style="background:#ABCDEF;" align="center"|'''Function''' || osmoprotection
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''