GanA - Revision history

134.76.70.252 at 11:25, 17 April 2014

2014-04-17T11:25:32Z

134.76.38.147 at 14:48, 2 April 2014

2014-04-02T14:48:24Z

134.76.70.252 at 14:36, 16 May 2013

2013-05-16T14:36:57Z

134.76.70.252 at 11:18, 14 May 2013

2013-05-14T11:18:49Z

134.76.70.252 at 13:53, 13 May 2013

2013-05-13T13:53:28Z

134.76.70.252 at 12:19, 19 September 2012

2012-09-19T12:19:08Z

134.76.70.252 at 12:18, 19 September 2012

2012-09-19T12:18:59Z

Jstuelk at 13:53, 16 August 2012

2012-08-16T13:53:14Z

Jstuelk: moved LacA to GanA: 22893383

2012-08-16T13:31:02Z

moved LacA to GanA: 22893383

134.76.70.252 at 16:06, 7 August 2012

2012-08-07T16:06:11Z

← Older revision		Revision as of 11:25, 17 April 2014
Line 1:		Line 1:
−	* '''Description:~~''' beta-galactosidase <br/><br/>~~	+	{{#bank: GanA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''ganA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''yvfN, lacA ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| beta-galactosidase~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| galactan utilization~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34130 ganA]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 79 kDa, 5.704~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 2061 bp, 687 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[ganB]]'', ''[[ganQ]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU34130 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU34130 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU34130 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:lacA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ganA_3503020_3505083_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:lacA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34130]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[utilization of specific carbon sources]]}},~~
−	~~{{SubtiWiki category\|[[phosphoproteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[LacR regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU34130
−
−	~~===Phenotypes of a mutant ===~~
−	* a ''[[cycB]]-[[ganP]]-[[ganQ]]-[[ganA]]-[[ganB]]'' mutant is impaired in the utilization of galactan {{PubMed\|22893383}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU34130&redirect=T BSU34130]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yvfKLM-lacA-yvfO.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12439]
−
−	~~=== Additional information===~~
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−	** degradation of galactotriose to galactose {{PubMed\|22893383}}
−
−	* '''Protein family:''' glycosyl hydrolase 42 family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−	** phosphorylated on Arg-387 {{PubMed\|22517742}}
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU34130&redirect=T BSU34130]
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/O07012 O07012]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU34130]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/3.2.1.23 3.2.1.23] 3
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−	* '''Operon:''' ''[[cycB]]-[[ganP]]-[[ganQ]]-[[ganA]]-[[ganB]]'' {{PubMed\|9287030}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ganA_3503020_3505083_-1 ganA] {{PubMed\|22383849}}
−
−	* '''Sigma factor:'''
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−	** [[LacR]]: transcription repression {{PubMed\|9287030}}
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''GP1181 (aphA3) available in the [[Stülke]] lab
−	** 1A785 ( ''ganA''::''spec''), {{PubMed\|11274134}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A785&Search=1A785 BGSC]
−	** 1A786 ( ''ganA''::''spec''), {{PubMed\|11274134}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A786&Search=1A786 BGSC]
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−
−	~~<pubmed>2104611,9287030,17056685 , 22517742 22893383</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 55: / Line 55: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yvfKLM-lacA-yvfO.html]
@@ Line 92: / Line 93: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 27: / Line 27: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ganA_3503020_3505083_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:lacA_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ganA_3503020_3505083_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:lacA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34130]]
 |-
 |}

@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ganB]]'', ''[[ganQ]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU34130 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU34130 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU34130 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU34130 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU34130 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU34130 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:lacA_context.gif]]

@@ Line 14: / Line 14: @@
 |style="background:#ABCDEF;" align="center"|'''Function''' || galactan utilization
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU34130 ganA]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34130 ganA]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 79 kDa, 5.704
@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ganB]]'', ''[[ganQ]]''
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15418&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU34130 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU34130 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU34130 Advanced_DNA]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:lacA_context.gif]]

@@ Line 121: / Line 121: @@
 * '''Mutant:'''GP1181 (aphA3) available in the [[Stülke]] lab
 ** 1A785 ( ''ganA''::''spec''), {{PubMed|11274134}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A785&Search=1A785 BGSC]
 * '''Expression vector:'''

← Older revision		Revision as of 12:18, 19 September 2012
Line 120:		Line 120:

	* '''Mutant:'''GP1181 (aphA3) available in the [[Stülke]] lab		* '''Mutant:'''GP1181 (aphA3) available in the [[Stülke]] lab
		+	** 1A785 ( ''ganA''::''spec''), {{PubMed\|11274134}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A785&Search=1A785 BGSC]

	* '''Expression vector:'''		* '''Expression vector:'''

@@ Line 4: / Line 4: @@
 |-
 |style="background:#ABCDEF;" align="center"|'''Gene name'''
-|''lacA''
+|''ganA''
 |-
-|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yvfN ''
+|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yvfN, lacA ''
 |-
 |style="background:#ABCDEF;" align="center"| '''Essential''' || no
@@ Line 14: / Line 14: @@
 |style="background:#ABCDEF;" align="center"|'''Function''' || galactan utilization
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU34130 lacA]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU34130 ganA]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 79 kDa, 5.704
@@ Line 20: / Line 20: @@
 |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2061 bp, 687 aa
 |-
-|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yvfO]]'', ''[[yvfM]]''
+|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ganB]]'', ''[[ganQ]]''
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15418&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
@@ Line 32: / Line 32: @@
 __TOC__
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/>
@@ Line 55: / Line 52: @@
 ===Phenotypes of a mutant ===
 === Database entries ===
@@ Line 63: / Line 61: @@
 === Additional information===
@@ Line 71: / Line 67: @@
 === Basic information/ Evolution ===
-* '''Catalyzed reaction/ biological activity:''' Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides (according to Swiss-Prot)
+* '''Catalyzed reaction/ biological activity:'''
 * '''Protein family:''' glycosyl hydrolase 42 family (according to Swiss-Prot)
@@ Line 107: / Line 104: @@
 =Expression and regulation=
-* '''Operon:''' ''[[cycB]]-[[yvfL]]-[[yvfM]]-[[lacA]]-[[yvfO]]'' {{PubMed|9287030}}
+* '''Operon:'''  ''[[cycB]]-[[ganP]]-[[ganQ]]-[[ganA]]-[[ganB]]'' {{PubMed|9287030}}
-* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ganA_3503020_3505083_-1 lacA] {{PubMed|22383849}}
+* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ganA_3503020_3505083_-1 ganA] {{PubMed|22383849}}
 * '''Sigma factor:'''
@@ Line 140: / Line 137: @@
 =References=
-<pubmed>2104611,9287030,17056685 , 22517742</pubmed>
+<pubmed>2104611,9287030,17056685 , 22517742 22893383</pubmed>
 [[Category:Protein-coding genes]]

← Older revision	Revision as of 13:31, 16 August 2012
(No difference)