FusA - Revision history

Jstuelk: /* References */

2014-11-26T11:51:33Z

‎References

Jstuelk at 17:17, 7 May 2014

2014-05-07T17:17:27Z

134.76.70.252 at 14:06, 17 April 2014

2014-04-17T14:06:44Z

134.76.70.252 at 09:49, 17 April 2014

2014-04-17T09:49:54Z

134.76.38.147 at 12:49, 2 April 2014

2014-04-02T12:49:21Z

Jstuelk at 12:53, 5 March 2014

2014-03-05T12:53:15Z

134.76.70.252 at 15:00, 11 November 2013

2013-11-11T15:00:54Z

Jstuelk: /* Extended information on the protein */

2013-08-20T11:29:13Z

‎Extended information on the protein

Jstuelk at 11:28, 20 August 2013

2013-08-20T11:28:22Z

134.76.70.252 at 12:22, 16 May 2013

2013-05-16T12:22:23Z

← Older revision		Revision as of 11:51, 26 November 2014
Line 1:		Line 1:
−	* '''Description:~~''' elongation factor G, facilitates movement of tRNA–mRNA by one codon <br/><br/>~~	+	{{#bank: FusA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''fusA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''fus ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| elongation factor G~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| [[translation]]~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01120 fusA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=FusA FusA]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 76 kDa, 4.615~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 2076 bp, 692 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[rpsG]]'', ''[[tufA]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU01120 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU01120 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU01120 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:fusA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fusA_130684_132762_1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:fusA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01120]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[translation]]}},~~
−	~~{{SubtiWiki category\|[[essential genes]]}},~~
−	~~{{SubtiWiki category\|[[phosphoproteins]]}},~~
−	~~[[most abundant proteins]]~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[stringent response]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU01120
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU01120&redirect=T BSU01120]
−
−	* '''DBTBS entry:''' no entry
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11939]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' hydrolyses GTP
−
−	* '''Protein family:''' EF-G/EF-2 subfamily (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:''' phosphorylation on Ser-213 AND Ser-302 AND Ser-569 AND Ser-680 AND (Thr-24 OR Thr-25) AND (Thr-43 OR Ser 48) [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed]
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[FusA]]-[[RplL]] {{PubMed\|23912278}}
−	** [[FusA]]-[[RpsL]] {{PubMed\|23912278}}
−
−	* '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU01120&redirect=T BSU01120]
−
−	* '''Structure:'''
−	** [http://www.rcsb.org/pdb/explore/explore.do?pdbId=1ELO 1ELO] (from ''Thermus thermophilus'') {{PubMed\|8070397}}
−	** [http://www.pdb.org/pdb/explore/explore.do?structureId=4BTC 4BTC], [http://www.pdb.org/pdb/explore/explore.do?structureId=4BTD 4BTD], the ''Thermus thermophilus'' EF-G–[[ribosome]] complex in a pretranslocation state {{PubMed\|23912278}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P80868 P80868]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU01120]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:'''
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fusA_130684_132762_1 fusA] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−
−	* '''Regulation:'''
−	** [[RelA]] dependent downregulation (Class I) during stringent response {{PubMed\|11948165}}
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** belongs to the 100 [[most abundant proteins]] {{PubMed\|15378759}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 11452 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 49524 {{PubMed\|24696501}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 23259 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 12858 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 16612 {{PubMed\|21395229}}
−
−	~~=Biological materials =~~
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−	** for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP840, available in [[Jörg Stülke]]'s lab
−	** for expression/ purification from ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP848, available in [[Jörg Stülke]]'s lab
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−
−	~~<pubmed> 24614227 17726680, 17218307 20348921 8070397 23912278 15378759</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 152: / Line 152: @@
 =References=
-<pubmed> 17726680, 17218307 20348921 8070397 23912278 15378759</pubmed>
+<pubmed> 24614227 17726680, 17218307 20348921 8070397 23912278 15378759</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 127: / Line 127: @@
 ** number of protein molecules per cell (minimal medium with glucose and ammonium): 11452 {{PubMed|24696501}}
 ** number of protein molecules per cell (complex medium with amino acids, without glucose): 49524 {{PubMed|24696501}}
 =Biological materials =
 * '''Mutant:'''

@@ Line 125: / Line 125: @@
 * '''Additional information:'''
 ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
 =Biological materials =

@@ Line 59: / Line 59: @@
 === Database entries ===
 * '''DBTBS entry:''' no entry
@@ Line 95: / Line 96: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 42: / Line 42: @@
 {{SubtiWiki category|[[translation]]}},
 {{SubtiWiki category|[[essential genes]]}},
-{{SubtiWiki category|[[phosphoproteins]]}}
+{{SubtiWiki category|[[phosphoproteins]]}},
 = This gene is a member of the following [[regulons]] =
@@ Line 64: / Line 65: @@
 === Additional information===
 =The protein=
@@ Line 82: / Line 80: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:''' phosphorylation on Ser-213 AND Ser-302 AND Ser-569 AND Ser-680 AND (Thr-24 OR Thr-25) AND (Thr-43 OR Ser 48) [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed]
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 116: / Line 114: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fusA_130684_132762_1 fusA] {{PubMed|22383849}}
-* '''Sigma factor:'''
+* '''[[Sigma factor]]:'''
 * '''Regulation:'''
@@ Line 124: / Line 122: @@
 * '''Additional information:'''
 =Biological materials =
@@ Line 147: / Line 146: @@
 =References=
-<pubmed> 17726680, 17218307 20348921 8070397 23912278</pubmed>
+<pubmed> 17726680, 17218307 20348921 8070397 23912278 15378759</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01120 fusA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/FusA FusA]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=FusA FusA]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 76 kDa, 4.615

@@ Line 91: / Line 91: @@
 * '''[[SubtInteract|Interactions]]:'''
-** FusA-RplL {{PubMed|23912278}}
+** [[FusA]]-[[RplL]] {{PubMed|23912278}}
-** FusA-RpsL {{PubMed|23912278}}
+** [[FusA]]-[[RpsL]] {{PubMed|23912278}}
 * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)

@@ Line 1: / Line 1: @@
-* '''Description:''' elongation factor G <br/><br/>
+* '''Description:''' elongation factor G, facilitates movement of tRNA–mRNA by one codon <br/><br/>
 {| align="right" border="1" cellpadding="2"
@@ Line 12: / Line 12: @@
 |style="background:#ABCDEF;" align="center"| '''Product''' || elongation factor G
 |-
-|style="background:#ABCDEF;" align="center"|'''Function''' || translation
+|style="background:#ABCDEF;" align="center"|'''Function''' || [[translation]]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01120 fusA]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 76 kDa, 4.615
@@ Line 35: / Line 37: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/>
@@ Line 93: / Line 91: @@
 * '''[[SubtInteract|Interactions]]:'''
 * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
@@ Line 98: / Line 98: @@
 === Database entries ===
-* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=1ELO 1ELO] (from ''Thermus thermophilus'') {{PubMed|8070397}}
+* '''Structure:'''
 * '''UniProt:''' [http://www.uniprot.org/uniprot/P80868 P80868]
@@ Line 128: / Line 130: @@
 * '''Expression vector:'''
-** for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP840, available in [[Stülke]] lab
+** for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP840, available in [[Jörg Stülke]]'s lab
-** for expression/ purification from ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP848, available in [[Stülke]] lab
+** for expression/ purification from ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP848, available in [[Jörg Stülke]]'s lab
 * '''lacZ fusion:'''
@@ Line 145: / Line 147: @@
 =References=
-<pubmed> 17726680, 17218307 20348921 8070397 </pubmed>
+<pubmed> 17726680, 17218307 20348921 8070397 23912278</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 27: / Line 27: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fusA_130684_132762_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:fusA_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fusA_130684_132762_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:fusA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01120]]
 |-
 |}