Fra - Revision history

Jstuelk at 11:41, 1 April 2015

2015-04-01T11:41:57Z

Jstuelk: /* Extended information on the protein */

2015-04-01T11:15:50Z

‎Extended information on the protein

Jstuelk: /* Extended information on the protein */

2015-04-01T11:13:55Z

‎Extended information on the protein

Jstuelk: /* References */

2015-04-01T08:31:03Z

‎References

134.76.70.252 at 11:25, 17 April 2014

2014-04-17T11:25:10Z

134.76.70.252 at 11:53, 8 April 2014

2014-04-08T11:53:02Z

134.76.38.147 at 13:05, 2 April 2014

2014-04-02T13:05:25Z

134.76.70.252 at 14:14, 18 November 2013

2013-11-18T14:14:14Z

134.76.70.252 at 12:39, 16 May 2013

2013-05-16T12:39:51Z

134.76.70.252 at 09:35, 14 May 2013

2013-05-14T09:35:10Z

← Older revision		Revision as of 11:41, 1 April 2015
Line 1:		Line 1:
−	* '''Description:''' frataxin-like protein, required for the biosynthesis of iron-sulfur clusters <br/><br/>	+	{{#bank: Fra}}
−	{~~\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:~~#~~ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''fra''~~
−	\|-
−	~~\|style="background~~:~~#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''ydhG ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| frataxin-like protein~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| intracellular iron channeling, biosynthesis of iron-sulfur clusters~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU05750 fra]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=Fra ~~Fra]~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=Fra Fra]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 14 kDa, 5.377~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 369 bp, 123 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[phoB]]'', ''[[ydhH]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU05750 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU05750 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU05750 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:ydhG_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	~~\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ydhG_621847_622218_1 Expression at a glance]'''   {{PubMed\|22383849}~~}~~<br/>[[Image:fra_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU05750]]~~
−	\|-
−	\|}

−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−
−
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[iron metabolism]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU05750
−
−	~~===Phenotypes of a mutant ===~~
−	* severe growth phenotype associated with a broadly disturbed iron homeostasis {{PubMed\|21744456}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU05750&redirect=T BSU05750]
−
−	* '''DBTBS entry:''' no entry
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12184]
−
−	~~=== Additional information===~~
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' transfers iron onto the B. subtilis SUF system ([[SufU]]-[[SufS]]) for iron-sulfur cluster biosynthesis {{PubMed\|21744456}}
−
−	* '''Protein family:'''
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):''' Fe(2+) is bound with high affinity {{PubMed\|20087498}}
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** the protein is active as a dimer {{PubMed\|20087498}}
−	** ([[Fra]])2-[[SufU]], requires iron {{PubMed\|20087498}}
−	** ([[Fra]])-[[HemH]], requires iron, Kd of 1.63 ± 0.02 μM {{PubMed\|25826316}}
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU05750&redirect=T BSU05750]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2OC6 2OC6]
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/Q797E6 Q797E6]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU05750]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:'''
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ydhG_621847_622218_1 fra] {{PubMed\|22383849}}
−
−	* '''Sigma factor:'''
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 178 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 451 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:''' available in [[Mohamed Marahiel]]'s lab
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~<pubmed> 20087498 21744456 25826316</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 94: / Line 94: @@
 ** the protein is active as a dimer {{PubMed|20087498}}
 ** ([[Fra]])2-[[SufU]], requires iron {{PubMed|20087498}}
-** ([[Fra]])-[[HemH]], requires iron {{PubMed|25826316}}
+** ([[Fra]])-[[HemH]], requires iron, Kd of 1.63 ± 0.02 μM {{PubMed|25826316}}
 * '''[[Localization]]:'''

@@ Line 94: / Line 94: @@
 ** the protein is active as a dimer {{PubMed|20087498}}
 ** ([[Fra]])2-[[SufU]], requires iron {{PubMed|20087498}}
 * '''[[Localization]]:'''

@@ Line 145: / Line 145: @@
 =References=
-'''Additional publications:''' {{PubMed|21744456}}
+<pubmed> 20087498 21744456  25826316</pubmed>
 [[Category:Protein-coding genes]]

← Older revision		Revision as of 11:25, 17 April 2014
Line 123:		Line 123:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium): 178 {{PubMed\|24696501}}
		+	** number of protein molecules per cell (complex medium with amino acids, without glucose): 451 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

@@ Line 16: / Line 16: @@
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=Fra Fra]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 5.377

@@ Line 58: / Line 58: @@
 === Database entries ===
 * '''DBTBS entry:''' no entry
@@ Line 95: / Line 96: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2OC6 2OC6]

@@ Line 15: / Line 15: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU05750 fra]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/Fra Fra]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=Fra Fra]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 5.377

@@ Line 28: / Line 28: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ydhG_621847_622218_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:fra_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ydhG_621847_622218_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:fra_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU05750]]
 |-
 |}

@@ Line 23: / Line 23: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[phoB]]'', ''[[ydhH]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU05750 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU05750 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU05750 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU05750 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU05750 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU05750 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:ydhG_context.gif]]