FloT - Revision history

Jstuelk: /* Extended information on the protein */

2015-08-28T15:39:19Z

‎Extended information on the protein

Show changes

Jstuelk at 15:37, 28 August 2015

2015-08-28T15:37:14Z

Atkovacs at 11:41, 30 April 2015

2015-04-30T11:41:47Z

Atkovacs: /* Biological materials */

2015-04-30T11:37:11Z

‎Biological materials

Atkovacs at 11:35, 30 April 2015

2015-04-30T11:35:23Z

Jstuelk: /* Original publications */

2015-04-26T16:47:32Z

‎Original publications

Jstuelk: /* References */

2015-02-20T12:17:36Z

‎References

Jstuelk: /* References */

2015-01-31T20:24:35Z

‎References

134.76.70.252 at 11:24, 17 April 2014

2014-04-17T11:24:24Z

134.76.38.147 at 14:36, 2 April 2014

2014-04-02T14:36:18Z

@@ Line 1: / Line 1: @@
-* '''Description:''' similar to flotillin 1, orchestration of physiological processes in lipid microdomains, involved in the control of membrane fluidity, confers (together with [[YuaF]]) resistance to cefuroxime  <br/><br/>
+* '''Description:''' membrane-associated scaffold protein, orchestration of physiological processes in lipid microdomains, involved in the control of membrane fluidity, confers (together with [[YuaF]]) resistance to cefuroxime  <br/><br/>
 {| align="right" border="1" cellpadding="2"
@@ Line 10: / Line 10: @@
 |style="background:#ABCDEF;" align="center"| '''Essential''' || no
 |-
-|style="background:#ABCDEF;" align="center"| '''Product''' ||  bacterial flotillin-like protein
+|style="background:#ABCDEF;" align="center"| '''Product''' ||  membrane-associated scaffold protein
 |-
 |style="background:#ABCDEF;" align="center"|'''Function''' || involved in the control of membrane fluidity
@@ Line 102: / Line 102: @@
 ** oligomerization depends on the glutamate-alanine repeats {{PubMed|25909364}}
 ** [[YuaF]]-[[FloT]] {{PubMed|22753055}}
-** [[FloT]]-[[FtsH]] {{PubMed|22882210}}
+** [[FloT]]-[[FtsH]] {{PubMed|26297017,22882210}}
-** [[FloT]]-[[FloA]] {{PubMed|23651456}}
+** [[FloT]]-[[FloA]] {{PubMed|26297017,23651456}}
 ** [[FloT]]-[[SecY]] {{PubMed|23651456}}
 ** [[FloT]]-[[FtsX]] {{PubMed|23651456}}
 ** [[FloT]]-[[OppA]] {{PubMed|23651456}}
 ** [[FloT]]-[[SdhA]] {{PubMed|23651456}}
-** [[FloT]]-[[ResE]] {{PubMed|25909364}}
+** [[FloT]]-[[ResE]] {{PubMed|26297017,25909364}}
 * '''[[Localization]]:'''
@@ Line 173: / Line 175: @@
 <pubmed> 25652542</pubmed>
 == Original publications ==
-<pubmed>9987136,22753055, 12107147, 18763711, 19383680  20713508 23651456 22178969 ,23249255  22882210 25635948 25909364 24222488</pubmed>
+<pubmed>9987136,22753055, 12107147, 18763711, 19383680  20713508 23651456 22178969 ,23249255  22882210 25635948 25909364 24222488 26297017</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 161: / Line 161: @@
 ** JS166 (3610 ''lacA::floT''-PAmCherry (mls)), available in [[Daniel Lopez]]'s lab
-* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Daniel Lopez]]'s lab
+* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]) for FloT {{PubMed|25909364}}, available in [[Daniel Lopez]]'s lab
 * '''Antibody:'''

@@ Line 156: / Line 156: @@
 * '''GFP fusion:'''
-** GK38 (168 [[amyE]]::P[[floT-yfp]] (spc)), available in [[Daniel Lopez]]'s lab
+** GK38 (168 ''amyE''::P''floT-yfp'' (spc)), available in [[Daniel Lopez]]'s lab
-** JS280 (3610 [[amyE::floT-gfp]](spc)), available in [[Daniel Lopez]]'s lab
+** JS280 (3610 ''amyE::floT-gfp''(spc)), available in [[Daniel Lopez]]'s lab
-** JS153 (3610 [[lacA::floT]]-mEos2 (mls)), available in [[Daniel Lopez]]'s lab
+** JS153 (3610 ''lacA::floT''-mEos2 (mls)), available in [[Daniel Lopez]]'s lab
-** JS166 (3610 [[lacA::floT]]-PAmCherry (mls)), available in [[Daniel Lopez]]'s lab
+** JS166 (3610 ''lacA::floT''-PAmCherry (mls)), available in [[Daniel Lopez]]'s lab
 * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Daniel Lopez]]'s lab

@@ Line 62: / Line 62: @@
 ** a ''[[floT]] [[floA]]'' double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency {{PubMed|22753055}}
 ** a ''[[floT]] [[floA]]'' double mutant has a [[sporulation]] defect, due to the lack of [[FtsH]] {{PubMed|22882210}}
 === Database entries ===
@@ Line 99: / Line 100: @@
 * '''[[SubtInteract|Interactions]]:'''
 ** forms homo-oligomers {{PubMed|23651456}}
 ** [[YuaF]]-[[FloT]] {{PubMed|22753055}}
 ** [[FloT]]-[[FtsH]] {{PubMed|22882210}}
@@ Line 106: / Line 108: @@
 ** [[FloT]]-[[OppA]] {{PubMed|23651456}}
 ** [[FloT]]-[[SdhA]] {{PubMed|23651456}}
 * '''[[Localization]]:'''
@@ Line 111: / Line 114: @@
 ** co-localizes with [[FloA]] and [[KinC]] in discrete foci membrane {{PubMed|20713508}}
 ** forms discrete focal structures in the cytoplasma membrane {{PubMed|22753055}}
 === Database entries ===
@@ Line 145: / Line 149: @@
 * '''Mutant:'''
 * '''Expression vector:'''
@@ Line 151: / Line 156: @@
 * '''GFP fusion:'''
-* '''two-hybrid system:'''
+* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Daniel Lopez]]'s lab
 * '''Antibody:'''

@@ Line 164: / Line 164: @@
 <pubmed> 25652542</pubmed>
 == Original publications ==
-<pubmed>9987136,22753055, 12107147, 18763711, 19383680  20713508 23651456 22178969 ,23249255  22882210 25635948
+<pubmed>9987136,22753055, 12107147, 18763711, 19383680  20713508 23651456 22178969 ,23249255  22882210 25635948 25909364 24222488</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 162: / Line 162: @@
 =References=
-<pubmed>9987136,22753055, 12107147, 18763711, 19383680  20713508 23651456 22178969 ,23249255  22882210 24222488</pubmed>
+<pubmed>9987136,22753055, 12107147, 18763711, 19383680  20713508 23651456 22178969 ,23249255  22882210 25635948
 [[Category:Protein-coding genes]]

← Older revision		Revision as of 11:24, 17 April 2014
Line 139:		Line 139:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium): 1215 {{PubMed\|24696501}}
		+	** number of protein molecules per cell (complex medium with amino acids, without glucose): 236 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

@@ Line 64: / Line 64: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yuaFGI.html]
@@ Line 112: / Line 113: @@
 === Database entries ===
 * '''Structure:'''