FloA - Revision history

Jstuelk at 15:43, 28 August 2015

2015-08-28T15:43:19Z

Atkovacs at 11:41, 30 April 2015

2015-04-30T11:41:53Z

Atkovacs: /* Biological materials */

2015-04-30T11:38:18Z

‎Biological materials

Atkovacs at 11:35, 30 April 2015

2015-04-30T11:35:13Z

Atkovacs at 11:08, 30 April 2015

2015-04-30T11:08:31Z

Jstuelk: /* Original publications */

2015-04-26T16:47:47Z

‎Original publications

Jstuelk at 16:41, 26 April 2015

2015-04-26T16:41:24Z

Jstuelk: /* References */

2015-02-20T12:17:48Z

‎References

134.76.70.252 at 11:24, 17 April 2014

2014-04-17T11:24:35Z

134.76.38.147 at 14:15, 2 April 2014

2014-04-02T14:15:36Z

← Older revision		Revision as of 15:43, 28 August 2015
Line 1:		Line 1:
−	* '''Description:~~''' flottilin-like protein (in addition to [[FloT]]), resistence protein (against sublancin), accessory role in resistance to cefuroxime<br/><br/>~~	+	{{#bank: FloA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''floA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' yqfA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| flottilin-like protein~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| resistance against sublancin~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=yqfA floA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=FloA FloA]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 35 kDa, 4.917~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 993 bp, 331 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[yqfB]]'', ''[[yqeZ]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25380 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25380 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25380 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:yqfA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yqfA_2617449_2618444_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:yqfA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25380]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[membrane dynamics]]}},~~
−	~~{{SubtiWiki category\|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}},~~
−	~~{{SubtiWiki category\|[[resistance against toxins/ antibiotics]]}},~~
−	~~{{SubtiWiki category\|[[membrane proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[SigW regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU25380
−
−	~~===Phenotypes of a mutant ===~~
−	** reduced [[protein secretion]] {{PubMed\|23651456}}
−	** a ''[[floT]] [[floA]]'' double mutant does not induce [[KinC]]-dependent biofilm formation upon addition of surfactin {{PubMed\|20713508}}
−	** a ''[[floT]] [[floA]]'' double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency {{PubMed\|22753055}}
−	** a ''[[floT]] [[floA]]'' double mutant has a [[sporulation]] defect, due to the lack of [[FtsH]] {{PubMed\|22882210}}
−	** a ''[[floA]]'' mutant produces increased amount of pulcherriminic acid {{PubMed\|25909364}}
−	** a ''[[floA]]'' mutant has reduced sensitivity to vancomycin {{PubMed\|25909364}}
−
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU25380&redirect=T BSU25380]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yqeZ-yqfA-yqfB.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11651]
−
−	~~=== Additional information===~~
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−	** controls protease activity of [[FtsH]] {{PubMed\|24222488}}
−
−	* '''Protein family:''' UPF0365 family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** oligomerization depends on the glutamate-alanine repeats {{PubMed\|25909364}}
−	** [[FloA]]-[[FtsH]] {{PubMed\|22882210}}
−	** [[FloT]]-[[FloA]] {{PubMed\|23651456}}
−	** [[FloA]]-[[PhoR]] {{PubMed\|25909364}}
−
−	* '''[[Localization]]:'''
−	** membrane [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
−	** co-localizes with [[FloT]] in discrete foci in the membrane {{PubMed\|20713508}}
−	** forms focal structure in the cytoplasma membrane {{PubMed\|22753055}}
−	** 13 foci [[FloA]] of are detected in strain 3610 {{PubMed\|25909364}}
−
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU25380&redirect=T BSU25380]
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P54466 P54466]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU25380]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[yqeZ]]-[[floA]]-[[yqfB]]'' {{PubMed\|11866510}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yqfA_2617449_2618444_-1 floA] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigW]] {{PubMed\|11866510}}
−
−	* '''Regulation:'''
−	** expressed under stress conditions ([[SigW]]) {{PubMed\|11866510}}
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 3975 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 3635 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** JS119 (3610 markerless), available in [[Daniel Lopez]]'s lab
−	** DL1401 (168 ''ΔfloA''::mls), available in [[Daniel Lopez]]'s lab
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−	** GK192 (3610 ''lacA''::P''floT-yfp''(mls)), available in [[Daniel Lopez]]'s lab
−	** JS136 (3610 ''amyE::floT''-gfp(spc)), available in [[Daniel Lopez]]'s lab
−	** JS134 (3610 ''lacA::floA''-mEos2 (mls)), available in [[Daniel Lopez]]'s lab
−	** JS167 (3610 ''lacA::floA''-PAmCherry (mls)), available in [[Daniel Lopez]]'s lab
−
−	* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]) for FloA {{PubMed\|25909364}}, available in [[Daniel Lopez]]'s lab
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~== Reviews ==~~
−	~~<pubmed> 25652542</pubmed>~~
−	~~== Original publications ==~~
−	~~<pubmed>16629676,11866510,18763711, 20713508 22753055 22882210 23651456 22178969 24222488 25909364 </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 155: / Line 155: @@
 ** JS167 (3610 ''lacA::floA''-PAmCherry (mls)), available in [[Daniel Lopez]]'s lab
-* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Daniel Lopez]]'s lab
+* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]) for FloA {{PubMed|25909364}}, available in [[Daniel Lopez]]'s lab
 * '''Antibody:'''

@@ Line 143: / Line 143: @@
 * '''Mutant:'''
 ** JS119 (3610 markerless), available in [[Daniel Lopez]]'s lab
-** DL1401 (168 [[ΔfloA]]::mls), available in [[Daniel Lopez]]'s lab
+** DL1401 (168 ''ΔfloA''::mls), available in [[Daniel Lopez]]'s lab
 * '''Expression vector:'''
@@ Line 150: / Line 150: @@
 * '''GFP fusion:'''
-** GK192 (3610 [[lacA]]::P[[floT-yfp]](mls)), available in [[Daniel Lopez]]'s lab
+** GK192 (3610 ''lacA''::P''floT-yfp''(mls)), available in [[Daniel Lopez]]'s lab
-** JS136 (3610 [[amyE::floT]]-gfp(spc)), available in [[Daniel Lopez]]'s lab
+** JS136 (3610 ''amyE::floT''-gfp(spc)), available in [[Daniel Lopez]]'s lab
-** JS134 (3610 [[lacA::floA]]-mEos2 (mls)), available in [[Daniel Lopez]]'s lab
+** JS134 (3610 ''lacA::floA''-mEos2 (mls)), available in [[Daniel Lopez]]'s lab
-** JS167 (3610 [[lacA::floA]]-PAmCherry (mls)), available in [[Daniel Lopez]]'s lab
+** JS167 (3610 ''lacA::floA''-PAmCherry (mls)), available in [[Daniel Lopez]]'s lab
 * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Daniel Lopez]]'s lab

@@ Line 97: / Line 97: @@
 * '''[[SubtInteract|Interactions]]:'''
 ** [[FloA]]-[[FtsH]] {{PubMed|22882210}}
 ** [[FloT]]-[[FloA]] {{PubMed|23651456}}
 * '''[[Localization]]:'''
@@ Line 104: / Line 106: @@
 ** co-localizes with [[FloT]] in discrete foci in the membrane {{PubMed|20713508}}
 ** forms focal structure in the cytoplasma membrane {{PubMed|22753055}}
 === Database entries ===
@@ Line 138: / Line 142: @@
 * '''Mutant:'''
 * '''Expression vector:'''
@@ Line 144: / Line 150: @@
 * '''GFP fusion:'''
-* '''two-hybrid system:'''
+* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Daniel Lopez]]'s lab
 * '''Antibody:'''

@@ Line 59: / Line 59: @@
 ** a ''[[floT]] [[floA]]'' double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency {{PubMed|22753055}}
 ** a ''[[floT]] [[floA]]'' double mutant has a [[sporulation]] defect, due to the lack of [[FtsH]] {{PubMed|22882210}}
 === Database entries ===

@@ Line 154: / Line 154: @@
 <pubmed> 25652542</pubmed>
 == Original publications ==
-<pubmed>16629676,11866510,18763711, 20713508 22753055 22882210 23651456 22178969 24222488
+<pubmed>16629676,11866510,18763711, 20713508 22753055 22882210 23651456 22178969 24222488 25909364 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 14: / Line 14: @@
 |style="background:#ABCDEF;" align="center"|'''Function''' || resistance against sublancin
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/yqfA/ floA]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=yqfA floA]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=FloA FloA]

← Older revision		Revision as of 11:24, 17 April 2014
Line 129:		Line 129:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium): 3975 {{PubMed\|24696501}}
		+	** number of protein molecules per cell (complex medium with amino acids, without glucose): 3635 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

@@ Line 61: / Line 61: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yqeZ-yqfA-yqfB.html]
@@ Line 102: / Line 103: @@
 === Database entries ===
 * '''Structure:'''