EpsA - Revision history

Jstuelk: /* Original publications */

2015-08-19T17:14:50Z

‎Original publications

Jstuelk at 15:30, 19 August 2015

2015-08-19T15:30:20Z

Jstuelk at 09:21, 5 August 2014

2014-08-05T09:21:06Z

134.76.38.147 at 14:50, 2 April 2014

2014-04-02T14:50:15Z

Jstuelk: /* Biological materials */

2014-02-06T12:54:49Z

‎Biological materials

Jstuelk at 12:00, 6 February 2014

2014-02-06T12:00:25Z

JGerwig: /* Biological materials */

2014-01-31T11:34:55Z

‎Biological materials

Jstuelk at 10:29, 31 January 2014

2014-01-31T10:29:49Z

JGerwig: /* Biological materials */

2014-01-28T08:36:48Z

‎Biological materials

JGerwig: /* Biological materials */

2014-01-28T08:34:57Z

‎Biological materials

← Older revision		Revision as of 17:14, 19 August 2015
Line 1:		Line 1:
−	* '''Description:~~''' extracellular polysaccharide synthesis, putative transmembrane modulator of [[EpsB]] activity, might activate [[EpsB]] autophosphorylation and substrate phosphorylation <br/><br/>~~	+	{{#bank: EpsA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''epsA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''yveK ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| unknown~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| [[biofilm formation]]~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34370 epsA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=EpsA EpsA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=epsA epsA]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 25 kDa, 6.071~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 702 bp, 234 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[epsB]]'', ''[[slrR]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU34370 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU34370 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU34370 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:yveK_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=epsA_3529151_3529855_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:epsA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34370]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[protein modification]]}},~~
−	~~{{SubtiWiki category\|[[biofilm formation]]}},~~
−	~~{{SubtiWiki category\|[[membrane proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[AbrB regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[RemA regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[SinR regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU34370
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU34370&redirect=T BSU34370]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/eps.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11859]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−
−	* '''Protein family:''' cpsC/capA family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):''' [[TkmA]]
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[EpsA]]-[[EpsB]] {{PubMed\|24493247}}
−
−	* '''[[Localization]]:''' cell membrane (according to Swiss-Prot)
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU34370&redirect=T BSU34370]
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P71050 P71050]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU34370]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−	* '''Operon:''' ''[[epsA]]-[[epsB]]-[[epsC]]-[[epsD]]-[[epsE]]-[[epsF]]-[[epsG]]-[[epsH]]-[[epsI]]-[[epsJ]]-[[epsK]]-[[epsL]]-[[epsM]]-[[epsN]]-[[epsO]]'' {{PubMed\|15661000}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=epsA_3529151_3529855_-1 epsA] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigA]] {{PubMed\|15661000}}
−
−	* '''Regulation:'''
−	** repressed by [[SinR]] {{PubMed\|15661000}}
−
−	* '''Regulatory mechanism:'''
−	** [[SinR]]: transcription anti-activation (prevents binding of [[RemA]]) {{PubMed\|23646920}}
−	** [[RemA]]: transcription activation {{PubMed\|23646920}}
−	** [[AbrB]]: transcription repression {{PubMed\|20817675}}
−
−	* '''Additional information:'''
−	** induction by sequestration of [[SinR]] by [[SinI]] or [[SlrA]] {{PubMed\|15661000,19788541}} or by SlrR {{PubMed\|20351052}}
−	** the ''[[epsA]]-[[epsB]]-[[epsC]]-[[epsD]]-[[epsE]]-[[epsF]]-[[epsG]]-[[epsH]]-[[epsI]]-[[epsJ]]-[[epsK]]-[[epsL]]-[[epsM]]-[[epsN]]-[[epsO]]'' operon is not expressed in a ''[[ymdB]]'' mutant {{PubMed\|21856853}}
−	** the amount of the mRNA is substantially decreased upon depletion of [[Rny\|RNase Y]] (this is likely due to the increased stability of the ''[[sinR]]'' mRNA) {{PubMed\|21815947}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** GP1517 (aphA3) {{PubMed\|24493247}}, available in [[ Jörg Stülke]]'s lab
−	** GP1519 (''[[epsA]]-[[epsB]]'', aphA3) {{PubMed\|24493247}}, available in [[ Jörg Stülke]]'s lab
−	** GP1567 ''[[epsA]]''::aphA3 ''[[tkmA]]''::spc {{PubMed\|24493247}}, available in [[ Jörg Stülke]]'s lab
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:''' GP1569 epsA-gfp (spc), available in [[ Jörg Stülke]]'s lab
−
−	* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]) {{PubMed\|24493247}}, available in [[ Jörg Stülke]]'s lab
−
−	* '''Antibody:'''
−
−	* '''FLAG-tag construct:''' GP1526 epsA-FLAG 3x spc (based on [[pGP1331]]) {{PubMed\|24493247}}, available in [[ Jörg Stülke]]'s lab
−
−	~~=Labs working on this gene/protein=~~
−
−	~~[[Richard Losick]], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed>20735481 </pubmed>~~
−
−	~~==Original publications==~~
−	~~<pubmed>15661000,16430695,18047568,18647168 20351052 20817675 21856853 21815947 23646920 24493247 25085422 </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 91: / Line 91: @@
 * '''[[SubtInteract|Interactions]]:'''
-** [[EpsA]]-[[EpsB]] (based on [[TkmA]]-[[PtkA]] interaction)
+** [[EpsA]]-[[EpsB]] {{PubMed|24493247}}
 * '''[[Localization]]:''' cell membrane (according to Swiss-Prot)

@@ Line 158: / Line 158: @@
 ==Original publications==
-<pubmed>15661000,16430695,18047568,18647168 20351052 20817675 21856853 21815947 23646920 24493247 </pubmed>
+<pubmed>15661000,16430695,18047568,18647168 20351052 20817675 21856853 21815947 23646920 24493247 25085422 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 60: / Line 60: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/eps.html]
@@ Line 95: / Line 96: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 129: / Line 129: @@
 * '''Mutant:'''
-** GP1517 (aphA3), available in [[ Jörg Stülke]]'s lab
+** GP1517 (aphA3) {{PubMed|24493247}}, available in [[ Jörg Stülke]]'s lab
-** GP1519 (''[[epsA]]-[[epsB]]'', aphA3), available in [[ Jörg Stülke]]'s lab
+** GP1519 (''[[epsA]]-[[epsB]]'', aphA3) {{PubMed|24493247}}, available in [[ Jörg Stülke]]'s lab
-** GP1567 ''[[epsA]]''::aphA3 ''[[tkmA]]''::spc, available in [[ Jörg Stülke]]'s lab
+** GP1567 ''[[epsA]]''::aphA3 ''[[tkmA]]''::spc {{PubMed|24493247}}, available in [[ Jörg Stülke]]'s lab
 * '''Expression vector:'''
@@ Line 137: / Line 137: @@
 * '''lacZ fusion:'''
-* '''GFP fusion:''' GP1569 epsA-gfp (spc)
+* '''GFP fusion:''' GP1569 epsA-gfp (spc), available in [[ Jörg Stülke]]'s lab
-* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[ Jörg Stülke]]'s lab
+* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]) {{PubMed|24493247}}, available in [[ Jörg Stülke]]'s lab
 * '''Antibody:'''
-* '''FLAG-tag construct:''' GP1526 epsA-FLAG 3x spc (based on [[pGP1331]]) available in [[ Jörg Stülke]]'s lab
+* '''FLAG-tag construct:''' GP1526 epsA-FLAG 3x spc (based on [[pGP1331]]) {{PubMed|24493247}}, available in [[ Jörg Stülke]]'s lab
 =Labs working on this gene/protein=

@@ Line 66: / Line 66: @@
 === Additional information===
 =The protein=
@@ Line 84: / Line 81: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 132: / Line 129: @@
 * '''Mutant:'''
-** GP1517 (aphA3), available in [[Stülke]] lab
+** GP1517 (aphA3), available in [[ Jörg Stülke]]'s lab
-** GP1519 (epsAB, aphA3), available in [[Stülke]] lab
+** GP1519 (''[[epsA]]-[[epsB]]'', aphA3), available in [[ Jörg Stülke]]'s lab
-** GP1567 epsA::aphA3 tkmA::spc, available in [[Stülke]] lab
+** GP1567 ''[[epsA]]''::aphA3 ''[[tkmA]]''::spc, available in [[ Jörg Stülke]]'s lab
 * '''Expression vector:'''
@@ Line 142: / Line 139: @@
 * '''GFP fusion:''' GP1569 epsA-gfp (spc)
-* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
+* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[ Jörg Stülke]]'s lab
 * '''Antibody:'''
-* '''FLAG-tag construct:''' GP1526 epsA-FLAG 3x spc available in [[Stülke]] lab
+* '''FLAG-tag construct:''' GP1526 epsA-FLAG 3x spc available in [[ Jörg Stülke]]'s lab
 =Labs working on this gene/protein=