CysJ - Revision history

Jstuelk at 16:42, 17 April 2015

2015-04-17T16:42:08Z

134.76.70.252 at 14:22, 17 April 2014

2014-04-17T14:22:26Z

134.76.70.252 at 09:25, 17 April 2014

2014-04-17T09:25:22Z

134.76.38.147 at 14:45, 2 April 2014

2014-04-02T14:45:06Z

Jstuelk at 16:24, 5 March 2014

2014-03-05T16:24:27Z

134.76.70.252 at 11:39, 7 January 2014

2014-01-07T11:39:50Z

134.76.70.252 at 14:34, 16 May 2013

2013-05-16T14:34:14Z

134.76.70.252 at 11:15, 14 May 2013

2013-05-14T11:15:53Z

134.76.70.252 at 13:50, 13 May 2013

2013-05-13T13:50:37Z

134.76.70.252 at 12:05, 19 September 2012

2012-09-19T12:05:53Z

← Older revision		Revision as of 16:42, 17 April 2015
Line 1:		Line 1:
−	* '''Description:~~''' sulfite reductase (NADPH2) <br/><br/>~~	+	{{#bank: CysJ}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''cysJ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''yvgQ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| sulfite reductase (NADPH2)~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| sulfite reduction~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33440 cysJ]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=cysJ cysJ]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 64 kDa, 5.49~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1713 bp, 571 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[nhaK]]'', ''[[cysI]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU33440 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU33440 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU33440 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:yvgQ_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cysI_3430598_3432313_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:cysJ_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33440]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[sulfur metabolism]]}},~~
−	~~[[most abundant proteins]]~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[CysL regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU33430
−
−	~~===Phenotypes of a mutant ===~~
−	* unable to grow with sulfate or sulfite as the only sulfur source {{PubMed\|12169591}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU33440&redirect=T BSU33440]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yvgRQ.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG14099]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−
−	* '''Protein family:'''
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:''' contains an iron-sulfur cluster
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU33440&redirect=T BSU33440]
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/O32214 O32214]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU33430]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−	* '''Operon:''' ''[[cysI]]-[[cysJ]]'' {{PubMed\|12169591}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cysI_3430598_3432313_-1 cysJ] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigA]] {{PubMed\|12169591}}
−
−	* '''Regulation:'''
−	** induced by sulfite, sulfate, and thiosulfate ([[CysL]]) {{PubMed\|12169591}}
−	** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]
−
−	* '''Regulatory mechanism:'''
−	** [[CysL]]: transcription activation {{PubMed\|12169591}}
−
−	* '''Additional information:'''
−	** belongs to the 100 [[most abundant proteins]] {{PubMed\|15378759}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 2899 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 1652 {{PubMed\|24696501}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1863 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 824 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 899 {{PubMed\|21395229}}
−
−	~~=Biological materials =~~
−	* '''Mutant:'''
−	** 1A802 ( ''cysJ''::''kan''), {{PubMed\|11445163}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A802&Search=1A802 BGSC]
−	** 1A934 ( ''cysJ''::''kan''), {{PubMed\|12169591}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A934&Search=1A934 BGSC]
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−
−	~~<pubmed>15378759,12169591,12107147, 11445163 </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 121: / Line 121: @@
 ** number of protein molecules per cell (minimal medium with glucose and ammonium): 2899 {{PubMed|24696501}}
 ** number of protein molecules per cell (complex medium with amino acids, without glucose): 1652 {{PubMed|24696501}}
 =Biological materials =
 * '''Mutant:'''
 ** 1A802 ( ''cysJ''::''kan''), {{PubMed|11445163}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A802&Search=1A802 BGSC]

@@ Line 119: / Line 119: @@
 * '''Additional information:'''
 ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
 =Biological materials =

@@ Line 56: / Line 56: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yvgRQ.html]
@@ Line 90: / Line 91: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 37: / Line 37: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
-<br/><br/><br/><br/>
+<br/><br/>
 = [[Categories]] containing this gene/protein =
-{{SubtiWiki category|[[sulfur metabolism]]}}
+{{SubtiWiki category|[[sulfur metabolism]]}},
 = This gene is a member of the following [[regulons]] =
@@ Line 65: / Line 62: @@
 === Additional information===
 =The protein=
@@ Line 83: / Line 77: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
-* '''Cofactor(s):''' contains an iron-sulfur cluster
+* '''[[Cofactors]]:''' contains an iron-sulfur cluster
 * '''Effectors of protein activity:'''
@@ Line 112: / Line 106: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cysI_3430598_3432313_-1 cysJ] {{PubMed|22383849}}
-* '''Sigma factor:''' [[SigA]] {{PubMed|12169591}}
+* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|12169591}}
 * '''Regulation:'''
@@ Line 122: / Line 116: @@
 * '''Additional information:'''
 =Biological materials =
@@ Line 145: / Line 140: @@
 =References=
-<pubmed>,12169591,12107147, 11445163 </pubmed>
+<pubmed>15378759,12169591,12107147, 11445163 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33440 cysJ]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/cys_meth_and_sulfate_assimilation.html Cys, Met & Sulfate assimilation]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=cysJ cysJ]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 64 kDa, 5.49

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cysI_3430598_3432313_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:cysJ_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cysI_3430598_3432313_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:cysJ_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33440]]
 |-
 |}

@@ Line 24: / Line 24: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nhaK]]'', ''[[cysI]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU33440 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU33440 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU33440 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU33440 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU33440 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU33440 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:yvgQ_context.gif]]