CshB - Revision history

Jstuelk at 11:50, 10 November 2015

2015-11-10T11:50:12Z

2014-04-17T09:47:34Z

2014-04-02T14:14:28Z

2013-11-18T14:18:43Z

2013-05-16T13:56:32Z

2013-05-14T10:44:33Z

2013-05-13T13:19:34Z

2012-11-26T16:47:07Z

2012-11-24T13:00:45Z

2012-09-20T12:09:17Z

‎Biological materials

← Older revision		Revision as of 11:50, 10 November 2015
Line 1:		Line 1:
		+	{{#bank: cshB}}
	* '''Description:''' [[DEAD-box RNA helicases\|DEAD-box RNA helicase]], important for adaptation to low temperatures<br/><br/>		* '''Description:''' [[DEAD-box RNA helicases\|DEAD-box RNA helicase]], important for adaptation to low temperatures<br/><br/>

← Older revision		Revision as of 09:47, 17 April 2014
Line 123:		Line 123:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium): 141 {{PubMed\|24696501}}
		+	** number of protein molecules per cell (complex medium with amino acids, without glucose): 526 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

@@ Line 56: / Line 56: @@
 === Database entries ===
 * '''DBTBS entry:''' no entry
@@ Line 95: / Line 96: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25140 cshB]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CshB CshB]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=CshB CshB]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 49 kDa, 9.89

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cshB_2594184_2595500_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:cshB_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cshB_2594184_2595500_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:cshB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25140]]
 |-
 |}

@@ Line 24: / Line 24: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nfo]]'', ''[[yqfQ]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25140 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25140 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25140 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25140 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25140 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25140 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:yqfR_context.gif]]

@@ Line 1: / Line 1: @@
-* '''Description:''' [[DEAD-box RNA helicases|DEAD-box RNA helicase]]<br/><br/>
+* '''Description:''' [[DEAD-box RNA helicases|DEAD-box RNA helicase]], important for adaptation to low temperatures<br/><br/>
 {| align="right" border="1" cellpadding="2"
@@ Line 68: / Line 68: @@
 === Basic information/ Evolution ===
-* '''Catalyzed reaction/ biological activity:''' RNA helicase
+* '''Catalyzed reaction/ biological activity:'''
 * '''Protein family:''' [[DEAD-box RNA helicases|DEAD-box RNA helicase]]
@@ Line 114: / Line 116: @@
 * '''Regulation:'''
 ** well expressed in complex medium, poorly expressed in minimal medium {{PubMed|20572937}}
-** expression declines in stationary phase {{PubMed|20572937}}
+** expression declines in stationary phase {{PubMed|23175651,20572937}}
 * '''Regulatory mechanism:'''

@@ Line 37: / Line 37: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/>
 = [[Categories]] containing this gene/protein =
-{{SubtiWiki category|[[DEAD-box RNA helicases]]}}
+{{SubtiWiki category|[[DEAD-box RNA helicases]]}},
 = This gene is a member of the following [[regulons]] =
@@ Line 56: / Line 53: @@
 ===Phenotypes of a mutant ===
 === Database entries ===
@@ Line 64: / Line 62: @@
 === Additional information===
@@ Line 114: / Line 110: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cshB_2594184_2595500_-1 cshB] {{PubMed|22383849}}
-* '''Sigma factor:'''
+* '''[[Sigma factor]]:'''
 * '''Regulation:'''
@@ Line 150: / Line 146: @@
 =References=
 '''Additional publications:''' {{PubMed|20709848}}
 [[Category:Protein-coding genes]]

@@ Line 126: / Line 126: @@
 =Biological materials =
-* '''Mutant:''' GP1051 (cat), available in [[Stülke]] lab
+* '''Mutant:'''
 * '''Expression vector:'''
+** for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [[SPINE]], expression from the native chromomsomal site: GP1064 (spc), available in [[Jörg Stülke]]'s lab
 * '''lacZ fusion:'''
 * '''GFP fusion:'''
-* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
+* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
-* '''FLAG-tag construct:''' GP1011 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab
+* '''FLAG-tag construct:'''
 * '''Antibody:'''