Crh - Revision history

134.76.38.147 at 14:52, 2 April 2014

2014-04-02T14:52:13Z

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Jstuelk: /* Biological materials */

2014-01-16T17:46:34Z

‎Biological materials

Jstuelk: /* Biological materials */

2014-01-16T17:45:01Z

‎Biological materials

134.76.70.252 at 11:44, 7 January 2014

2014-01-07T11:44:57Z

134.76.70.252 at 09:25, 12 November 2013

2013-11-12T09:25:11Z

134.76.70.252 at 14:39, 16 May 2013

2013-05-16T14:39:48Z

134.76.70.252 at 11:21, 14 May 2013

2013-05-14T11:21:51Z

134.76.70.252 at 13:56, 13 May 2013

2013-05-13T13:56:28Z

134.76.70.252 at 16:19, 7 August 2012

2012-08-07T16:19:41Z

134.76.70.252 at 09:58, 23 April 2012

2012-04-23T09:58:02Z

@@ Line 136: / Line 136: @@
 =Biological materials =
-* '''Mutant:''' GP860 (aphA3) {{PubMed|21992469}}, QB7097 (spc), available in [[Stülke]] lab
+* '''Mutant:''' GP860 (aphA3) {{PubMed|21992469}}, QB7097 (spc), available in [[Jörg Stülke]]'s lab
 * '''Expression vector:'''
-** pGP641 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab
+** pGP641 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Jörg Stülke]]'s lab
-** pGP734 (C-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP382]]), available in [[Stülke]] lab
+** pGP734 (C-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP382]]), available in [[Jörg Stülke]]'s lab
 * '''lacZ fusion:''' see ''[[yvcI]]''
@@ Line 146: / Line 146: @@
 * '''GFP fusion:'''
-* '''two-hybrid system:''' ''[[crh]]'', ''crh(Ser46Asp)'', ''crh(Ser46Ala)'' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Boris Görke| Görke]] lab
+* '''two-hybrid system:''' ''[[crh]]'', ''crh(Ser46Asp)'', ''crh(Ser46Ala)'' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
 * '''FLAG-tag construct:
-** GP69 (spc, based on [[pGP1331]]) {{PubMed|21992469}}, available in the [[Boris Görke]] and [[Stülke]] labs
+** GP69 (spc, based on [[pGP1331]]) {{PubMed|21992469}}, available in [[Jörg Stülke]]'s lab
-** pGP1200 ''crh''(Ser46Ala) (spc, based on [[pGP1331]]), available in the [[Stülke]] lab
+** pGP1200 ''crh''(Ser46Ala) (spc, based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab
 =Labs working on this gene/protein=

@@ Line 136: / Line 136: @@
 =Biological materials =
-* '''Mutant:''' GP860 (aphA3), QB7097 (spc), available in [[Stülke]] lab
+* '''Mutant:''' GP860 (aphA3) {{PubMed|21992469}}, QB7097 (spc), available in [[Stülke]] lab
 * '''Expression vector:'''
@@ Line 149: / Line 149: @@
 * '''FLAG-tag construct:
-** GP69 (spc, based on [[pGP1331]]), available in the [[Boris Görke]] and [[Stülke]] labs
+** GP69 (spc, based on [[pGP1331]]) {{PubMed|21992469}}, available in the [[Boris Görke]] and [[Stülke]] labs
 ** pGP1200 ''crh''(Ser46Ala) (spc, based on [[pGP1331]]), available in the [[Stülke]] lab
 =Labs working on this gene/protein=

@@ Line 18: / Line 18: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=Crh Crh]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=crh crh]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 9,2 kDa, 4.70

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34740 crh]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/Crh Crh]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=Crh Crh]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''

@@ Line 31: / Line 31: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=crh_3569292_3569549_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:crh_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=crh_3569292_3569549_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:crh_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34740]]
 |-
 |}

@@ Line 26: / Line 26: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yvcN]]'', ''[[yvcL]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU34740 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU34740 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU34740 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU34740 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU34740 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU34740 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:crh_context.gif]]

@@ Line 13: / Line 13: @@
 |-
 |style="background:#ABCDEF;" align="center"|'''Function''' || control of carbon flux
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/Crh Crh]