CdaS - Revision history

Jstuelk: /* References */

2015-04-17T15:51:32Z

‎References

Jstuelk at 06:33, 19 June 2014

2014-06-19T06:33:09Z

134.76.70.252 at 11:39, 8 April 2014

2014-04-08T11:39:16Z

134.76.38.147 at 13:55, 2 April 2014

2014-04-02T13:55:23Z

Jstuelk at 18:27, 4 December 2013

2013-12-04T18:27:26Z

Jstuelk: /* Biological materials */

2013-08-13T18:01:15Z

‎Biological materials

Fmehne: /* Biological materials */

2013-08-13T16:45:15Z

‎Biological materials

Fmehne: /* Biological materials */

2013-08-13T16:42:42Z

‎Biological materials

Fmehne: /* Biological materials */

2013-08-13T16:41:48Z

‎Biological materials

134.76.70.252 at 13:34, 16 May 2013

2013-05-16T13:34:28Z

← Older revision		Revision as of 15:51, 17 April 2015
Line 1:		Line 1:
−	* '''Description:~~''' sporulation-specific diadenylate cyclase, synthesis of c-di-AMP<br/><br/>~~	+	{{#bank: CdaS}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''cdaS''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''yojJ ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| sporulation-specific diadenylate cyclase~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| synthesis of c-di-AMP~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU19430 cdaS]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=CdaS CdaS]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 22 kDa, 5.367~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 621 bp, 207 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[yojK]]'', ''[[yojI]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU19430 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU19430 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU19430 DNA_with_flanks]
−	\|-
−	\|-
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:yojJ_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yojJ_2118504_2119127_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:yojJ_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU19430]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[metabolism of signalling nucleotides]]}},~~
−	~~{{SubtiWiki category\|[[sporulation proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[SigG regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU19430
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU19430&redirect=T BSU19430]
−
−	* '''DBTBS entry:''' no entry
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13562]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−	** synthesis of c-di-AMP from two molecules of ATP {{PubMed\|23192352}}
−
−	* '''Protein family:'''
−
−	* '''Paralogous protein(s):''' [[CdaA]]
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−	** N-terminal autoinhibitory domain {{PubMed\|24939848}}
−	** C-terminal [[DAC domain]] involved in the synthesis of c-di-AMP {{PubMed\|21566650}}
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** forms hexamers (enzymatically inactive) or dimers (active) {{PubMed\|24939848}}
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU19430&redirect=T BSU19430]
−
−	* '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=2FB5 2FB5] (the protein of ''B. cereus'', 48% identity, 81% similarity)
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/O31854 O31854]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU19430]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[cdaS]]'' {{PubMed\|22383849}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yojJ_2118504_2119127_-1 cdaS] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigG]] {{PubMed\|22383849}}
−
−	* '''Regulation:'''
−	** expressed late during sporulation in the forespore ([[SigG]]) {{PubMed\|22383849}}
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** GP983 (''[[cdaS]]''::''ermC''), available in [[Jörg Stülke]]'s lab
−	** GP1360 (''[[cdaS]]''::''spc''), available in [[Jörg Stülke]]'s lab
−
−	* '''Expression vector:'''
−	** IPTG inducible expression of '''''B. cereus''''' Strep-''cdaS'' in ''E. coli'': pGP2593 (in [[pGP172]]), available in [[Jörg Stülke]]'s lab
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''His-tag fusion:'''
−	**pGP2591 10xHis-''cdaS'' under the control of native promoter (based on [[pAC7]]), available in [[Jörg Stülke]]'s lab
−	**pGP2592 10xHis-''cdaS-L44F'' under the control of native promoter (based on [[pAC7]]), available in [[Jörg Stülke]]'s lab
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed> 18714086</pubmed>~~
−	~~==Original publications==~~
−	~~<pubmed>21566650, 23192352 22383849 24939848 </pubmed>~~
−	~~[[Category:Protein-coding genes]]~~

@@ Line 81: / Line 81: @@
 * '''[[Domains]]:'''
-** contains a [[DAC domain]] involved in the synthesis of c-di-AMP {{PubMed|21566650}}
+** N-terminal autoinhibitory domain {{PubMed|24939848}}
 * '''Modification:'''
@@ Line 90: / Line 91: @@
 * '''[[SubtInteract|Interactions]]:'''
+** forms hexamers (enzymatically inactive) or dimers (active)  {{PubMed|24939848}}
 * '''[[Localization]]:'''
@@ Line 150: / Line 151: @@
 <pubmed> 18714086</pubmed>
 ==Original publications==
-<pubmed>21566650, 23192352 22383849</pubmed>
+<pubmed>21566650, 23192352 22383849 24939848 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 15: / Line 15: @@
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU19430 cdaS]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 22 kDa, 5.367

@@ Line 55: / Line 55: @@
 === Database entries ===
 * '''DBTBS entry:''' no entry
@@ Line 91: / Line 92: @@
 === Database entries ===
 * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=2FB5 2FB5] (the protein of ''B. cereus'', 48% identity, 81% similarity)

@@ Line 77: / Line 77: @@
 * '''Kinetic information:'''
-* '''Domains:''' contains a DAC domain involved in the synthesis of c-di-AMP {{PubMed|21566650}}
+* '''[[Domains]]:'''
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''

@@ Line 130: / Line 130: @@
 * '''His-tag fusion:'''
-**pGP2591 10xHis-''cdaS'' under the control of native promoter (based on [[pAC7]]), available in [[Jörg Stülke]]'s lab**
+**pGP2591 10xHis-''cdaS'' under the control of native promoter (based on [[pAC7]]), available in [[Jörg Stülke]]'s lab
-**pGP2592 10xHis-''cdaS-L44F'' under the control of native promoter (based on [[pAC7]]), available in [[Jörg Stülke]]'s lab**
+**pGP2592 10xHis-''cdaS-L44F'' under the control of native promoter (based on [[pAC7]]), available in [[Jörg Stülke]]'s lab
 * '''two-hybrid system:'''

← Older revision		Revision as of 16:45, 13 August 2013
Line 123:		Line 123:

	* '''Expression vector:'''		* '''Expression vector:'''
		+	** IPTG inducible expression of '''''B. cereus''''' Strep-''cdaS'' in ''E. coli'': pGP2593 (in [[pGP172]]), available in [[Jörg Stülke]]'s lab

	* '''lacZ fusion:'''		* '''lacZ fusion:'''

@@ Line 119: / Line 119: @@
 * '''Mutant:'''
-** GP983 (''[[yojJ]]''::''ermC''), available in [[Jörg Stülke]]'s lab
+** GP983 (''[[cdaS]]''::''ermC''), available in [[Jörg Stülke]]'s lab
-** GP1360 (''[[yojJ]]''::''spc''), available in [[Jörg Stülke]]'s lab
+** GP1360 (''[[cdaS]]''::''spc''), available in [[Jörg Stülke]]'s lab
 * '''Expression vector:'''

← Older revision		Revision as of 16:41, 13 August 2013
Line 127:		Line 127:

	* '''GFP fusion:'''		* '''GFP fusion:'''
		+
		+	* '''His-tag fusion:'''
		+	pGP2591 10xHis-''cdaS'' under the control of native promoter (based on [[pAC7]]), available in [[Jörg Stülke]]'s lab
		+	pGP2592 10xHis-''cdaS-L44F'' under the control of native promoter (based on [[pAC7]]), available in [[Jörg Stülke]]'s lab

	* '''two-hybrid system:'''		* '''two-hybrid system:'''

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yojJ_2118504_2119127_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:yojJ_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yojJ_2118504_2119127_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:yojJ_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU19430]]
 |-
 |}