Jstuelk at 13:06, 4 July 2013

2013-07-04T13:06:25Z

Jstuelk at 12:10, 3 July 2013

2013-07-03T12:10:33Z

Jstuelk: Created page with "This medium is used to grow B. subtilis in the presence of glucose or other variable carbon sources. The medium does contain ammonium and is therefore not suited to change"

2013-07-02T11:18:39Z

Created page with "This medium is used to grow B. subtilis in the presence of glucose or other variable carbon sources. The medium does contain ammonium and is therefore not suited to change"

New page

This medium is used to grow B. subtilis in the presence of glucose or other variable carbon sources. The medium does contain ammonium and is therefore not suited to change

← Older revision		Revision as of 13:06, 4 July 2013
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	== A published reference ==		== A published reference ==
	<pubmed> 18326565 </pubmed>		<pubmed> 18326565 </pubmed>
		+
		+	== Back to [[Methods]] ==

← Older revision		Revision as of 12:10, 3 July 2013
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−	This medium is used to grow B. subtilis in the presence of glucose or other variable carbon sources. The medium does contain ammonium and is therefore not suited to change	+	This medium is used to grow ''B. subtilis'' in the presence of glucose or other variable carbon sources. The medium does contain ammonium and is therefore not suited to change the nitrogen source.
		+
		+	== The basal solutions ==
		+	* 5 X C salts
		+	** 20 g KH2PO4
		+	** 80 g K2HPO4 X 3 H2O
		+	** 16.5 g (NH4)2SO4
		+	** Fill up to 1000 ml with deionised H2O
		+	* III’ salts
		+	** 0.232 g MnSO4 X 4 H2O
		+	** 12.3 g MgSO4 X 7 H2O
		+	** 12.5 ml of a ZnCl2 solution (100 mM)
		+	** Fill up to 1000 ml with deionised H2O
		+	* tryptophan (5 mg/ml)
		+	* Iron Ferric Ammonium Citrate (CAF; 2.2 mg/ml)
		+	* Potassium Glutamate (40 %)
		+	* Sodium Succinate (30 %)
		+
		+	== Preparation of the medium ==
		+	* 1 X C minimal medium
		+	** 20 ml 5 X C salts
		+	** 1 ml Tryptophan (5 mg/ml)
		+	** 1 ml Iron Ferric Ammonium Citrate (CAF; 2.2 mg/ml)
		+	** 1 ml III’ salts
		+	** Fill up to 100 ml with deionised H2O
		+
		+	* 1 X CSE minimal medium
		+	** 20 ml 5 X C salts
		+	** 1 ml Tryptophan (5 mg/ml)
		+	** 1 ml Iron Ferric Ammonium Citrate (CAF; 2.2 mg/ml)
		+	** 1 ml III’ salts
		+	** 2 ml Potassium Glutamate (40 %)
		+	** 2 ml Sodium Succinate (30 %)
		+	** Fill up to 100 ml with deionised H2O
		+
		+	* 1 X CSE Glc minimal medium
		+	** is CSE minimal medium with 0.5% Glucose
		+
		+	* 1 X CS minimal medium
		+	** is CSE minimal medium without Glutamate
		+
		+	* 1 X CS Glc minimal medium
		+	** is CS minimal medium with 0.5% Glucose
		+
		+	* 1 X C Glc minimal medium
		+	** is C minimal medium with 0.5% Glucose
		+
		+	== A published reference ==
		+	<pubmed> 18326565 </pubmed>

C minimal medium - Revision history

Jstuelk at 13:06, 4 July 2013

Jstuelk at 12:10, 3 July 2013

Jstuelk: Created page with "This medium is used to grow B. subtilis in the presence of glucose or other variable carbon sources. The medium does contain ammonium and is therefore not suited to change"