BslA - Revision history

Atkovacs at 11:21, 23 July 2015

2015-07-23T11:21:46Z

Jstuelk: /* Original publications */

2015-07-13T14:04:46Z

‎Original publications

Jstuelk: /* Reviews */

2015-04-26T15:36:30Z

‎Reviews

Jstuelk: /* Original publications */

2015-04-17T17:08:39Z

‎Original publications

Jstuelk: /* Reviews */

2014-07-07T08:15:27Z

‎Reviews

134.76.38.147 at 14:36, 2 April 2014

2014-04-02T14:36:30Z

Atkovacs: /* Expression and regulation */

2013-11-08T15:36:03Z

‎Expression and regulation

Jstuelk: /* Reviews */

2013-08-28T15:37:19Z

‎Reviews

Jstuelk: /* Biological materials */

2013-08-02T07:59:13Z

‎Biological materials

Jstuelk: /* Original publications */

2013-08-02T07:58:25Z

‎Original publications

← Older revision		Revision as of 11:21, 23 July 2015
Line 1:		Line 1:
−	* '''Description:~~''' bacterial hydrophobin, forms water-repellent surface layer of the biofilm, inhibitor of [[KinA]] autophosphorylation, and subsequently of entry into [[sporulation]] <br/><br/>~~	+	{{#bank: BslA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''bslA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''yuaB, sivB ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| biofilm surface layer, inhibitor of [[KinA]] autophosphorylation~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| [[biofilm formation]], control of entry into [[sporulation]] via the [[phosphorelay]]~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31080/ bslA]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 19 kDa, 9.987~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 543 bp, 181 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[gbsR]]'', ''[[ktrA]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31080 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31080 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31080 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:yuaB_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yuaB_3187503_3188048_1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:yuaB_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31080]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[biofilm formation]]}},~~
−	~~{{SubtiWiki category\|[[phosphorelay]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[AbrB regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[DegU regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU31080
−
−	~~===Phenotypes of a mutant ===~~
−	* reduced colony complexity {{PubMed\|21742882}}
−	* loss of surface repellency of the biofilm {{PubMed\|22571672}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU31080&redirect=T BSU31080]
−
−	* '''DBTBS entry:''' no entry
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12341]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−	** inhibits [[KinA]] autophophorylation {{PubMed\|23335417}}
−
−	* '''Protein family:'''
−
−	* '''Paralogous protein(s):''' [[SivA]]
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:'''
−	** extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
−	** biofilm matrix in an extracellular polysaccharide-dependent manner {{PubMed\|22571672}}
−	** forms a layer on the biofilm surface {{PubMed\|22571672}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU31080&redirect=T BSU31080]
−
−	* '''Structure:'''
−	** [http://www.rcsb.org/pdb/explore.do?structureId=4BHU 4BHU] {{PubMed\|23904481}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P71014 P71014]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU31080]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−	* '''Operon:''' ''[[bslA]]'' {{PubMed\|22383849}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yuaB_3187503_3188048_1 yuaB] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−	** [[AbrB]]: transcription repression {{PubMed\|18978066}}
−	** [[LutR]]: transcription activation {{PubMed\|24196425}}
−	** expression is down-regulated in a ''[[rok]]'' mutant (indirect effect) {{PubMed\|21097620}}
−	** [[DegU]]-P: transcription activation {{PubMed\|18978066}}, this might be indirect {{PubMed\|21742882}}
−
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** ''B. subtilis'' W939 ''bslA''::Cm (ATCC6051 based) {{PubMed\|17850253}}
−	** ''B. subtilis'' NRS2097 ''bslA''::Cm (NCIB3610 based) {{PubMed\|18978066}}
−	** ''B. subtilis'' ''bslA''::Cm (168 based) {{PubMed\|21097620}}
−
−	* '''Expression vector:'''
−	** Physpank-bslA based on vector pDR111: pDRyuaB2 {{PubMed\|21097620}}
−
−	* '''lacZ fusion:'''
−	** pNW500 P''bslA-lacZ'' fusion in pDG1663 {{PubMed\|18978066}}
−
−	* '''GFP fusion:'''
−	** P''bslA-gfp'' fusion in pSG1151 vector: pSGyuaB {{PubMed\|21097620}}
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed> 22607588 23979433 24988880 25907113</pubmed>~~
−
−	~~==Original publications==~~
−	~~<pubmed> 22571672 18978066 18957862 17850253 21097620 21742882 22383849 23335417 23904481 22571672 25870300 26152584</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 155: / Line 155: @@
 ==Original publications==
-<pubmed> 22571672 18978066 18957862 17850253 21097620 21742882 22383849 23335417 23904481 22571672 25870300</pubmed>
+<pubmed> 22571672 18978066 18957862 17850253 21097620 21742882 22383849 23335417 23904481 22571672 25870300 26152584</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 152: / Line 152: @@
 =References=
 ==Reviews==
-<pubmed> 22607588 23979433 24988880 </pubmed>
+<pubmed> 22607588 23979433 24988880 25907113</pubmed>
 ==Original publications==

@@ Line 155: / Line 155: @@
 ==Original publications==
-<pubmed> 22571672 18978066 18957862 17850253 21097620 21742882 22383849 23335417 23904481 22571672 </pubmed>
+<pubmed> 22571672 18978066 18957862 17850253 21097620 21742882 22383849 23335417 23904481 22571672 25870300</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 152: / Line 152: @@
 =References=
 ==Reviews==
-<pubmed> 22607588 23979433 </pubmed>
+<pubmed> 22607588 23979433 24988880 </pubmed>
 ==Original publications==

@@ Line 56: / Line 56: @@
 === Database entries ===
 * '''DBTBS entry:''' no entry
@@ Line 94: / Line 95: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 114: / Line 114: @@
 * '''Regulation:'''
 * '''Regulatory mechanism:'''
 ** [[DegU]]-P: transcription activation {{PubMed|18978066}}, this might be indirect {{PubMed|21742882}}
-** [[AbrB]]: transcription repression {{PubMed|18978066}}
 * '''Additional information:'''

@@ Line 125: / Line 125: @@
 * '''Mutant:'''
-** ''B. subtilis'' W939 ''yuaB''::Cm (ATCC6051 based) {{PubMed|17850253}}
+** ''B. subtilis'' W939 ''bslA''::Cm (ATCC6051 based) {{PubMed|17850253}}
-** ''B. subtilis'' NRS2097 ''yuaB''::Cm (NCIB3610 based) {{PubMed|18978066}}
+** ''B. subtilis'' NRS2097 ''bslA''::Cm (NCIB3610 based) {{PubMed|18978066}}
-** ''B. subtilis'' ''yuaB''::Cm (168 based) {{PubMed|21097620}}
+** ''B. subtilis'' ''bslA''::Cm (168 based) {{PubMed|21097620}}
 * '''Expression vector:'''
-** Physpank-yuaB based on vector pDR111: pDRyuaB2 {{PubMed|21097620}}
+** Physpank-bslA based on vector pDR111: pDRyuaB2 {{PubMed|21097620}}
 * '''lacZ fusion:'''
-** pNW500 P''yuaB-lacZ'' fusion in pDG1663 {{PubMed|18978066}}
+** pNW500 P''bslA-lacZ'' fusion in pDG1663 {{PubMed|18978066}}
 * '''GFP fusion:'''
-** P''yuaB-gfp'' fusion in pSG1151 vector: pSGyuaB {{PubMed|21097620}}
+** P''bslA-gfp'' fusion in pSG1151 vector: pSGyuaB {{PubMed|21097620}}
 * '''two-hybrid system:'''