BglS - Revision history

Jstuelk: /* References */

2015-03-27T09:09:03Z

‎References

Julian at 12:49, 24 April 2014

2014-04-24T12:49:41Z

Julian at 10:58, 24 April 2014

2014-04-24T10:58:03Z

Julian at 07:51, 22 April 2014

2014-04-22T07:51:34Z

Julian at 07:51, 22 April 2014

2014-04-22T07:51:09Z

134.76.38.147 at 15:10, 2 April 2014

2014-04-02T15:10:54Z

Jstuelk: /* References */

2014-03-25T08:01:26Z

‎References

Jstuelk at 12:22, 7 January 2014

2014-01-07T12:22:13Z

134.76.70.252 at 11:59, 7 January 2014

2014-01-07T11:59:52Z

134.76.70.252 at 14:57, 16 May 2013

2013-05-16T14:57:08Z

← Older revision		Revision as of 09:09, 27 March 2015
Line 1:		Line 1:
−	* '''Description:~~''' endo-beta-1,3-1,4 glucanase <br/><br/>~~	+	{{#bank: BglS}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''bglS''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''bgl, licS ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| endo-beta-1,3-1,4 glucanase~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| lichenan degradation~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU39070 bglS]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=bglS bglS]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 27 kDa, 6.482~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 726 bp, 242 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[citH]]'', ''[[licT]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU39070 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU39070 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU39070 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:bglS_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bglS_4011842_4012570_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:bglS_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU39070]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[utilization of specific carbon sources]]}},~~
−	~~{{SubtiWiki category\|[[sporulation proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[CcpA regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[LicT regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU39070
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU39070&redirect=T BSU39070]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/licT-bglS.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10476]
−
−	~~=== Additional information===~~
−
−
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds (according to Swiss-Prot)
−
−	* '''Protein family:''' glycosyl hydrolase 16 family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:''' extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU39070&redirect=T BSU39070]
−
−	* '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=3O5S 3O5S]
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P04957 P04957]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU39070]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/3.2.1.73 3.2.1.73]
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:'''
−	** ''[[licT]]-[[bglS]]'' {{PubMed\|8606172}}
−	** ''[[bglS]]'' {{PubMed\|8606172}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bglS_4011842_4012570_-1 bglS] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−	** ''[[licT]]'': [[SigA]] {{PubMed\|8606172}}
−
−	* '''Regulation:'''
−	** repressed by glucose (3.1-fold) ([[CcpA]]) {{PubMed\|12850135,8245831}}
−	** expressed in the stationary phase (temporal activation) {{PubMed\|8245830}}
−	** weak induction by ß-glucosides ([[LicT]]) {{PubMed\|8606172}}
−
−	* '''Regulatory mechanism:'''
−	** [[CcpA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/8245831 PubMed]
−	** [[LicT]]: binding to an [[RNA switch]] results in transcriptional antitermination {{PubMed\|8606172}}
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:''' GP427 (''[[licT]]-[[bglS]]'', erm), BGW7 (cat), both available in the [[Stülke]] lab
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−
−	~~<pubmed>18957862 12850135 6087283 8245830 8245831 8606172, 23459129 24391357 24659048 </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 41: / Line 41: @@
 = [[Categories]] containing this gene/protein =
 {{SubtiWiki category|[[utilization of specific carbon sources]]}},
-{{SubtiWiki category|[[Sporulation#Sporulation_proteins/_other|sporulation proteins]]}}
+{{SubtiWiki category|[[sporulation proteins]]}}
 = This gene is a member of the following [[regulons]] =

@@ Line 41: / Line 41: @@
 = [[Categories]] containing this gene/protein =
 {{SubtiWiki category|[[utilization of specific carbon sources]]}},
-{{SubtiWiki category|[[sporulation/ other]]}}
+{{SubtiWiki category|[[Sporulation#Sporulation_proteins/_other|sporulation proteins]]}}
 = This gene is a member of the following [[regulons]] =

@@ Line 40: / Line 40: @@
 = [[Categories]] containing this gene/protein =
-{{SubtiWiki category|[[utilization of specific carbon sources]]}}
+{{SubtiWiki category|[[utilization of specific carbon sources]]}},
 = This gene is a member of the following [[regulons]] =
 {{SubtiWiki regulon|[[CcpA regulon]]}},
-{{SubtiWiki regulon|[[LicT regulon]]}},
+{{SubtiWiki regulon|[[LicT regulon]]}}
 =The gene=

@@ Line 44: / Line 44: @@
 = This gene is a member of the following [[regulons]] =
 {{SubtiWiki regulon|[[CcpA regulon]]}},
-{{SubtiWiki regulon|[[LicT regulon]]}}
+{{SubtiWiki regulon|[[LicT regulon]]}},
 =The gene=

@@ Line 55: / Line 55: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/licT-bglS.html]
@@ Line 92: / Line 93: @@
 === Database entries ===
 * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=3O5S 3O5S]

@@ Line 145: / Line 145: @@
 =References=
-<pubmed>18957862 12850135 6087283 8245830 8245831 8606172, 23459129 24391357 </pubmed>
+<pubmed>18957862 12850135 6087283 8245830 8245831 8606172, 23459129 24391357 24659048 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 79: / Line 79: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 127: / Line 127: @@
 =Biological materials =
-* '''Mutant:''' GP427 (licT-bglS, erm), BGW7 (cat), both available in the [[Stülke]] lab
+* '''Mutant:''' GP427 (''[[licT]]-[[bglS]]'', erm), BGW7 (cat), both available in the [[Stülke]] lab
 * '''Expression vector:'''
@@ Line 145: / Line 145: @@
 =References=
-<pubmed>18957862 12850135 6087283 8245830 8245831 8606172, 23459129 </pubmed>
+<pubmed>18957862 12850135 6087283 8245830 8245831 8606172, 23459129 24391357 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU39070 bglS]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=bglS bglS]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 6.482

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bglS_4011842_4012570_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:bglS_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bglS_4011842_4012570_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:bglS_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU39070]]
 |-
 |}