BglC - Revision history

134.76.38.147 at 13:51, 2 April 2014

2014-04-02T13:51:14Z

134.76.70.252 at 13:29, 16 May 2013

2013-05-16T13:29:54Z

134.76.70.252 at 10:21, 14 May 2013

2013-05-14T10:21:43Z

134.76.70.252 at 12:57, 13 May 2013

2013-05-13T12:57:13Z

Jstuelk at 12:41, 2 April 2013

2013-04-02T12:41:59Z

134.76.70.252 at 10:42, 20 September 2012

2012-09-20T10:42:12Z

134.76.70.252 at 10:42, 20 September 2012

2012-09-20T10:42:02Z

134.76.70.252 at 10:41, 20 September 2012

2012-09-20T10:41:51Z

134.76.70.252 at 10:13, 7 August 2012

2012-08-07T10:13:35Z

134.76.70.252 at 10:31, 19 April 2012

2012-04-19T10:31:41Z

← Older revision		Revision as of 13:51, 2 April 2014
Line 1:		Line 1:
−	* '''Description:~~''' endo-1,4-beta-glucanase <br/><br/>~~	+	{{#bank: BglC}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''bglC''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''eglS ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| endo-1,4-beta-glucanase)~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| beta-1,4-glucan degradation~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU18130 bglC]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 55 kDa, 8.619~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1497 bp, 499 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[alsT]]'', ''[[ynfE]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU18130 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU18130 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU18130 DNA_with_flanks]
−	\|-
−	\|-
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:bglC_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=eglS_1940625_1942124_1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:bglC_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU18130]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[utilization of specific carbon sources]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU18130
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/bglC.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10437]
−
−	~~=== Additional information===~~
−
−
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans (according to Swiss-Prot)
−
−	* '''Protein family:''' glycosyl hydrolase 5 (cellulase A) family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:''' extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
−
−	~~=== Database entries ===~~
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3PZT 3PZT] {{PubMed\|21880019}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P10475 P10475]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU18130]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/3.2.1.4 3.2.1.4]
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''bglC'' (according to [http://dbtbs.hgc.jp/COG/prom/bglC.html DBTBS])
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=eglS_1940625_1942124_1 bglC] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigA]] {{PubMed\|3106328}}
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** 1A751 (no resistance), {{PubMed\|7704256}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A751&Search=1A751 BGSC]
−	** 1A752 (no resistance), {{PubMed\|7704256}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A752&Search=1A752 BGSC]
−	** 1A976 (no resistance), {{PubMed\|21255377}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A976&Search=1A976 BGSC]
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−
−	~~<pubmed>18957862,3024130 21880019 3106328 23543074 </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=eglS_1940625_1942124_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:bglC_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=eglS_1940625_1942124_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:bglC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU18130]]
 |-
 |}

@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[alsT]]'', ''[[ynfE]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU18130 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU18130 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU18130 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU18130 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU18130 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU18130 DNA_with_flanks]
 |-
 |-

@@ Line 14: / Line 14: @@
 |style="background:#ABCDEF;" align="center"|'''Function''' || beta-1,4-glucan degradation
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU18130 bglC]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU18130 bglC]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 55 kDa, 8.619
@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[alsT]]'', ''[[ynfE]]''
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12135&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU18130 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU18130 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU18130 Advanced_DNA]
 |-
 |-

@@ Line 37: / Line 37: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/>
@@ Line 112: / Line 108: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=eglS_1940625_1942124_1 bglC] {{PubMed|22383849}}
-* '''Sigma factor:''' [[SigA]] {{PubMed|3106328}}
+* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|3106328}}
 * '''Regulation:'''
@@ Line 143: / Line 139: @@
 =References=
-<pubmed>18957862,3024130 21880019 3106328 </pubmed>
+<pubmed>18957862,3024130 21880019 3106328 23543074 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 125: / Line 125: @@
 ** 1A751 (no resistance), {{PubMed|7704256}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A751&Search=1A751 BGSC]
 ** 1A752 (no resistance), {{PubMed|7704256}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A752&Search=1A752 BGSC]
 * '''Expression vector:'''

← Older revision		Revision as of 10:41, 20 September 2012
Line 123:		Line 123:

	* '''Mutant:'''		* '''Mutant:'''
		+	** 1A751 (no resistance), {{PubMed\|7704256}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A751&Search=1A751 BGSC]

	* '''Expression vector:'''		* '''Expression vector:'''