BacG - Revision history

134.76.70.252 at 11:24, 17 April 2014

2014-04-17T11:24:26Z

Jstuelk at 14:37, 27 March 2013

2013-03-27T14:37:27Z

Jstuelk at 14:32, 27 March 2013

2013-03-27T14:32:53Z

Jstuelk: Jstuelk moved page YwfH to BacG: 23519407

2013-03-27T14:28:12Z

Jstuelk moved page YwfH to BacG: 23519407

134.76.70.252 at 17:05, 7 August 2012

2012-08-07T17:05:40Z

134.76.70.252 at 11:31, 23 April 2012

2012-04-23T11:31:24Z

134.76.70.252 at 08:45, 17 April 2012

2012-04-17T08:45:24Z

Jstuelk at 17:01, 15 October 2011

2011-10-15T17:01:35Z

Jstuelk: /* References */

2011-09-29T19:22:28Z

‎References

Lflorez at 00:29, 9 December 2010

2010-12-09T00:29:29Z

← Older revision		Revision as of 11:24, 17 April 2014
Line 1:		Line 1:
−	* '''Description:~~''' short chain reductase involved in bacilysin synthesis<br/><br/>~~	+	{{#bank: BacG}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''bacG''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''ipa-86r, ywfH ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| short chain reductase~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| biosynthesis of the antibiotic bacilysin~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU37680 bacG]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 27 kDa, 6.596~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 777 bp, 259 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[hemQ]]'', ''[[bacF]]''~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15795]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:ywfH_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	~~\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywfH_3867493_3868272_1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:ywfH_expression.png\|500px]]~~
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[miscellaneous metabolic pathways]]}},~~
−	~~{{SubtiWiki category\|[[biosynthesis of antibacterial compounds]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[CodY regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU37680
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−
−	* '''DBTBS entry:''' no entry
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10632]
−
−	~~=== Additional information===~~
−
−
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−	** BacG catalyzes the conjugate addition of hydride at the C4 olefinic terminus using NADH to yield the cyclohexenol- containing tetrahydro-4-hydroxyphenylpyruvate {{PubMed\|20052993}}
−	** stereoselective reduction of dihydro-hydroxyphenylpyruvate (H2HPP) to tetrahydro-hydroxyphenylpyruvate (H4HPP), NADPH-dependent reductase that facilitates the conjugate addition of a hydride at the C(4) olefin terminus of H2HPP {{PubMed\|23519407}}
−
−	* '''Protein family:''' short-chain dehydrogenases/reductases (SDR) family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):''' NADPH {{PubMed\|23519407}}
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3u49 3U49] {{PubMed\|23519407}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P39644 P39644]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU37680]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''bacG'' {{PubMed\|12372825}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywfH_3867493_3868272_1 bacG] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−
−	* '''Regulation:'''
−	** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]
−
−	* '''Regulatory mechanism:'''
−	** [[CodY]]: transcription repression {{PubMed\|12372825}}
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−
−	~~<pubmed> 12372825 12107147 20052993 21948839 23519407 </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 4: / Line 4: @@
 |-
 |style="background:#ABCDEF;" align="center"|'''Gene name'''
-|''ywfH''
+|''bacG''
 |-
-|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipa-86r ''
+|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipa-86r, ywfH ''
 |-
 |style="background:#ABCDEF;" align="center"| '''Essential''' || no
@@ Line 14: / Line 14: @@
 |style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of the antibiotic bacilysin
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU37680 ywfH]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU37680 bacG]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 6.596
@@ Line 20: / Line 20: @@
 |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 777 bp, 259 aa
 |-
-|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywfI]]'', ''[[bacF]]''
+|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[hemQ]]'', ''[[bacF]]''
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15795&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
@@ Line 68: / Line 68: @@
 * '''Catalyzed reaction/ biological activity:'''
-** YwfH catalyzes the conjugate addition of hydride at the C4 olefinic terminus using NADH to yield the cyclohexenol- containing tetrahydro-4-hydroxyphenylpyruvate {{PubMed|20052993}}
+** BacG catalyzes the conjugate addition of hydride at the C4 olefinic terminus using NADH to yield the cyclohexenol- containing tetrahydro-4-hydroxyphenylpyruvate {{PubMed|20052993}}
 ** stereoselective reduction of dihydro-hydroxyphenylpyruvate (H2HPP) to tetrahydro-hydroxyphenylpyruvate (H4HPP), NADPH-dependent reductase that facilitates the conjugate addition of a hydride at the C(4) olefin terminus of H2HPP {{PubMed|23519407}}
@@ Line 83: / Line 83: @@
 * '''Modification:'''
-* '''Cofactor(s):''' NADPH
+* '''Cofactor(s):''' NADPH {{PubMed|23519407}}
 * '''Effectors of protein activity:'''
@@ Line 105: / Line 105: @@
 =Expression and regulation=
-* '''Operon:''' ''ywfH'' {{PubMed|12372825}}
+* '''Operon:''' ''bacG'' {{PubMed|12372825}}
-* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywfH_3867493_3868272_1 ywfH] {{PubMed|22383849}}
+* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywfH_3867493_3868272_1 bacG] {{PubMed|22383849}}
 * '''[[Sigma factor]]:'''

@@ Line 35: / Line 35: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/>
@@ Line 71: / Line 67: @@
 === Basic information/ Evolution ===
-* '''Catalyzed reaction/ biological activity:''' YwfH catalyzes the conjugate addition of hydride at the C4 olefinic terminus using NADH to yield the cyclohexenol- containing tetrahydro-4-hydroxyphenylpyruvate {{PubMed|20052993}}
+* '''Catalyzed reaction/ biological activity:'''
 * '''Protein family:''' short-chain dehydrogenases/reductases (SDR) family (according to Swiss-Prot)
@@ Line 85: / Line 83: @@
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''Cofactor(s):''' NADPH
 * '''Effectors of protein activity:'''
-* '''Interactions:'''
+* '''[[SubtInteract|Interactions]]:'''
-* '''Localization:'''
+* '''[[Localization]]:'''
 === Database entries ===
-* '''Structure:'''
+* '''Structure:'''	[http://www.rcsb.org/pdb/explore.do?structureId=3u49 3U49] {{PubMed|23519407}}
 * '''UniProt:''' [http://www.uniprot.org/uniprot/P39644 P39644]
@@ Line 111: / Line 109: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywfH_3867493_3868272_1 ywfH] {{PubMed|22383849}}
-* '''Sigma factor:'''
+* '''[[Sigma factor]]:'''
 * '''Regulation:'''
@@ Line 141: / Line 139: @@
 =References=
-<pubmed> 12372825  12107147 20052993 21948839</pubmed>
+<pubmed> 12372825  12107147 20052993 21948839 23519407 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 13: / Line 13: @@
 |-
 |style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of the antibiotic bacilysin
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 6.596

@@ Line 24: / Line 24: @@
 |colspan="2" | '''Genetic context''' <br/> [[Image:ywfH_context.gif]]
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
 |}
 __TOC__
 <br/><br/>

← Older revision	Revision as of 14:28, 27 March 2013
(No difference)

@@ Line 99: / Line 99: @@
 * '''Operon:''' ''ywfH'' {{PubMed|12372825}}
-* '''[[Sigma factor]]:'''
+* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywfH_3867493_3868272_1 ywfH] {{PubMed|22383849}}
 * '''Regulation:'''
@@ Line 107: / Line 109: @@
 ** [[CodY]]: transcription repression {{PubMed|12372825}}
 * '''Additional information:'''
 =Biological materials =

← Older revision		Revision as of 17:01, 15 October 2011
Line 1:		Line 1:
−	* '''Description:''' short chain reductase <br/><br/>	+	* '''Description:''' short chain reductase involved in bacilysin synthesis<br/><br/>

	{\| align="right" border="1" cellpadding="2"		{\| align="right" border="1" cellpadding="2"

@@ Line 30: / Line 30: @@
 <br/><br/>
 =The gene=
@@ Line 49: / Line 56: @@
-= Categories containing this gene/protein =
 =The protein=