Ald - Revision history

Jstuelk: /* Extended information on the protein */

2015-07-24T08:13:45Z

‎Extended information on the protein

Jstuelk: /* Database entries */

2015-07-24T08:12:20Z

‎Database entries

Jstuelk: /* References */

2015-07-24T08:11:56Z

‎References

134.76.70.252 at 14:02, 17 April 2014

2014-04-17T14:02:57Z

Jstuelk: Reverted edits by 134.76.70.252 (talk) to last revision by Jstuelk

2014-04-17T13:57:52Z

Reverted edits by 134.76.70.252 (talk) to last revision by Jstuelk

134.76.70.252 at 13:53, 17 April 2014

2014-04-17T13:53:25Z

Jstuelk: Reverted edits by 134.76.70.252 (talk) to last revision by 134.76.38.147

2014-04-17T13:43:02Z

Reverted edits by 134.76.70.252 (talk) to last revision by 134.76.38.147

134.76.70.252 at 13:32, 17 April 2014

2014-04-17T13:32:33Z

134.76.70.252 at 09:56, 17 April 2014

2014-04-17T09:56:58Z

134.76.38.147 at 14:39, 2 April 2014

2014-04-02T14:39:31Z

← Older revision		Revision as of 08:13, 24 July 2015
Line 1:		Line 1:
−	* '''Description:~~''' L-alanine dehydrogenase <br/><br/>~~	+	{{#bank: Ald}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''ald''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''spoVN ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| L-alanine dehydrogenase~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| alanine utilization~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31930 ald]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=ald ald]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 39 kDa, 5.125~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1134 bp, 378 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[adeR]]'', ''[[yukJ]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31930 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31930 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31930 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:ald_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ald_3278325_3279461_1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:ald_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31930]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/><br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[utilization of amino acids]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[AdeR regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU31930
−
−	~~===Phenotypes of a mutant ===~~
−	* [[sporulation]] defect {{PubMed\|22797752}}
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU31930&redirect=T BSU31930]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ald.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10468]
−
−	~~=== Additional information===~~
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' L-alanine + H<sub>2</sub>O + NAD<sup>+</sup> = pyruvate + NH<sub>3</sub> + NADH (according to Swiss-Prot)
−
−	* '''Protein family:''' AlaDH/PNT family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU31930&redirect=T BSU31930]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2EEZ 2EEZ] (from ''Thermus thermophilus hb8 mutant'', 55% identity, 66% similarity)
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/Q08352 Q08352]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU31930]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/1.4.1.1 1.4.1.1]
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[ald]]'' {{PubMed\|8226620}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ald_3278325_3279461_1 ald] {{PubMed\|22383849}}
−
−	* '''Sigma factor:''' [[SigA]] {{PubMed\|8226620}}
−
−	* '''Regulation:'''
−	** induced by alanine ([[AdeR]]) {{PubMed\|22797752,8226620}}
−
−	* '''Regulatory mechanism:'''
−	** [[AdeR]]: transcription activation {{PubMed\|22797752}}
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 15145 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 6392 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 15279 {{PubMed\|21395229}}
−
−	~~=Biological materials =~~
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~<pubmed>19114526,9168598,17183219,8226620, 22797752 26202482, </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

← Older revision		Revision as of 08:12, 24 July 2015
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	* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2EEZ 2EEZ] (from ''Thermus thermophilus hb8 mutant'', 55% identity, 66% similarity)		* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2EEZ 2EEZ] (from ''Thermus thermophilus hb8 mutant'', 55% identity, 66% similarity)
−

	* '''UniProt:''' [http://www.uniprot.org/uniprot/Q08352 Q08352]		* '''UniProt:''' [http://www.uniprot.org/uniprot/Q08352 Q08352]

@@ Line 140: / Line 140: @@
 =References=
-'''Additional publications:''' {{PubMed|22797752}}
+<pubmed>19114526,9168598,17183219,8226620, 22797752 26202482, </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 118: / Line 118: @@
 * '''Additional information:'''
 =Biological materials =
 * '''Mutant:'''

@@ Line 118: / Line 118: @@
 * '''Additional information:'''
-** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 6392 {{PubMed|21395229}}
+=Biological materials =
 * '''Mutant:'''

@@ Line 120: / Line 120: @@
 ** number of protein molecules per cell (minimal medium with glucose and ammonium): 845 {{PubMed|24696501}}
 ** number of protein molecules per cell (complex medium with amino acids, without glucose): 74423 {{PubMed|24696501}}
 =Biological materials =
 * '''Mutant:'''

← Older revision		Revision as of 09:56, 17 April 2014
Line 118:		Line 118:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium): 845 {{PubMed\|24696501}}
		+	** number of protein molecules per cell (complex medium with amino acids, without glucose): 74423 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

@@ Line 54: / Line 54: @@
 * [[sporulation]] defect {{PubMed|22797752}}
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ald.html]
@@ Line 89: / Line 90: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2EEZ 2EEZ] (from ''Thermus thermophilus hb8 mutant'', 55% identity, 66% similarity)