AcsA - Revision history

134.76.70.252 at 14:02, 17 April 2014

2014-04-17T14:02:46Z

Jstuelk: Reverted edits by 134.76.70.252 (talk) to last revision by Jstuelk

2014-04-17T13:57:42Z

Reverted edits by 134.76.70.252 (talk) to last revision by Jstuelk

134.76.70.252 at 13:53, 17 April 2014

2014-04-17T13:53:14Z

Jstuelk: Reverted edits by 134.76.70.252 (talk) to last revision by 134.76.38.147

2014-04-17T13:42:50Z

Reverted edits by 134.76.70.252 (talk) to last revision by 134.76.38.147

134.76.70.252 at 13:32, 17 April 2014

2014-04-17T13:32:21Z

134.76.70.252 at 09:54, 17 April 2014

2014-04-17T09:54:21Z

134.76.38.147 at 14:31, 2 April 2014

2014-04-02T14:31:42Z

134.76.70.252 at 11:28, 7 January 2014

2014-01-07T11:28:17Z

134.76.70.252 at 14:19, 16 May 2013

2013-05-16T14:19:21Z

134.76.70.252 at 11:01, 14 May 2013

2013-05-14T11:01:35Z

← Older revision		Revision as of 14:02, 17 April 2014
Line 1:		Line 1:
−	* '''Description:~~''' acetyl-CoA synthetase <br/><br/>~~	+	{{#bank: AcsA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''acsA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| acetyl-CoA synthetase)~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| utilization of acetate, fatty acids~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29680 acsA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=acsA acsA]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 64 kDa, 5.547~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1716 bp, 572 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[ytzK]]'', ''[[acuA]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29680 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29680 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29680 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:acsA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=acsA_3038213_3039931_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:acsA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29680]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−
−
−	~~<br/><br/><br/><br/><br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[utilization of specific carbon sources]]}},~~
−	~~{{SubtiWiki category\|[[utilization of lipids]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[CcpA regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[CodY regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU29680
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29680&redirect=T BSU29680]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/acsA.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10370]
−
−	~~=== Additional information===~~
−
−
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA (according to Swiss-Prot)
−
−	* '''Protein family:''' ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:''' acetylated on Lys-549 by [[AcuA]], this results in inactivation [http://www.ncbi.nlm.nih.gov/sites/entrez/18487328 PubMed], deacetylated by [[SrtN]] and [[AcuC]] deacetylates (and thereby activates) [[AcsA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19136592 PubMed]
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29680&redirect=T BSU29680]
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P39062 P39062]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29680]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/6.2.1.1 6.2.1.1]
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−	* '''Operon:''' ''[[acsA]]'' {{PubMed\|7934817}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=acsA_3038213_3039931_-1 acsA] {{PubMed\|22383849}}
−
−	* '''Sigma factor:''' [[SigA]] {{PubMed\|7913927}}
−
−	* '''Regulation:'''
−	** repressed by glucose (4.5-fold) ([[CcpA]]) {{PubMed\|7913927,12850135}}
−	** repressed during growth in the presence of branched chain amino acids ([[CodY]]) {{PubMed\|12618455,18083814}}
−
−	* '''Regulatory mechanism:'''
−	** [[CcpA]]: transcription repression {{PubMed\|7913927}}
−	** [[CodY]]: transcription repression {{PubMed\|12618455,18083814}}
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** GP1212 (''acsA''::''kan''), available in [[Stülke]] lab
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed> 15316652 15491124 15221478 </pubmed>~~
−
−	~~==Original publications==~~
−	~~<pubmed>7934817,7913927,18083814,17303131,12618455,19136592, 18487328, 12850135 16855235 22900538</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 126: / Line 126: @@
 * '''Additional information:'''
-** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1384 {{PubMed|21395229}}
+=Biological materials =
 * '''Mutant:'''
 ** GP1212 (''acsA''::''kan''), available in [[Stülke]] lab

@@ Line 126: / Line 126: @@
 * '''Additional information:'''
 =Biological materials =
 * '''Mutant:'''
 ** GP1212 (''acsA''::''kan''), available in [[Stülke]] lab

@@ Line 126: / Line 126: @@
 * '''Additional information:'''
-** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 3490 {{PubMed|21395229}}
+=Biological materials =
 * '''Mutant:'''
 ** GP1212 (''acsA''::''kan''), available in [[Stülke]] lab

@@ Line 128: / Line 128: @@
 ** number of protein molecules per cell (minimal medium with glucose and ammonium): 467 {{PubMed|24696501}}
 ** number of protein molecules per cell (complex medium with amino acids, without glucose): 3357 {{PubMed|24696501}}
 =Biological materials =
 * '''Mutant:'''
 ** GP1212 (''acsA''::''kan''), available in [[Stülke]] lab

← Older revision		Revision as of 09:54, 17 April 2014
Line 126:		Line 126:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium): 467 {{PubMed\|24696501}}
		+	** number of protein molecules per cell (complex medium with amino acids, without glucose): 3357 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

@@ Line 60: / Line 60: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/acsA.html]
@@ Line 97: / Line 98: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29680 acsA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=acsA acsA]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 64 kDa, 5.547

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=acsA_3038213_3039931_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:acsA_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=acsA_3038213_3039931_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:acsA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29680]]
 |-
 |}

@@ Line 24: / Line 24: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ytzK]]'', ''[[acuA]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29680 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29680 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29680 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29680 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29680 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29680 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:acsA_context.gif]]