AccC - Revision history

Dtoedter: /* Biological materials */

2015-04-02T11:43:59Z

‎Biological materials

Dtoedter: /* Biological materials */

2015-03-06T10:20:45Z

‎Biological materials

Jstuelk: /* References */

2014-10-10T07:49:50Z

‎References

Jstuelk: /* Reviews */

2014-10-10T07:49:30Z

‎Reviews

Jstuelk at 16:39, 5 June 2014

2014-06-05T16:39:46Z

134.76.70.252 at 14:02, 17 April 2014

2014-04-17T14:02:32Z

Jstuelk: Reverted edits by 134.76.70.252 (talk) to last revision by Jstuelk

2014-04-17T13:57:28Z

Reverted edits by 134.76.70.252 (talk) to last revision by Jstuelk

134.76.70.252 at 13:52, 17 April 2014

2014-04-17T13:52:58Z

Jstuelk: Reverted edits by 134.76.70.252 (talk) to last revision by 134.76.38.147

2014-04-17T13:42:35Z

Reverted edits by 134.76.70.252 (talk) to last revision by 134.76.38.147

134.76.70.252 at 13:32, 17 April 2014

2014-04-17T13:32:07Z

← Older revision		Revision as of 11:43, 2 April 2015
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−	* '''Description:~~''' acetyl-CoA carboxylase (biotin carboxylase subunit) <br/><br/>~~	+	{{#bank: AccC}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''accC''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''yqhX ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| acetyl-CoA carboxylase (biotin carboxylase subunit)~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| production of malonyl-CoA, the substrate for fatty acid biosynthesis~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU24340 accC]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=AccC AccC]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=accC accC]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 49 kDa, 4.72~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1350 bp, 450 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[yqhY]]'', ''[[accB]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU24340 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU24340 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU24340 DNA_with_flanks]
−	\|-
−	\|-
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:accC_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=accC_2530354_2531706_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:accC_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU24340]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[biosynthesis of lipids]]}},~~
−	~~{{SubtiWiki category\|[[essential genes]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU24340
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU24340&redirect=T BSU24340]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/accBC-yqhY.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11384]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' ATP + biotin-carboxyl-carrier protein + CO<sub>2</sub> = ADP + phosphate + carboxybiotin-carboxyl-carrier protein (according to Swiss-Prot)
−
−	* '''Protein family:'''
−
−	* '''Paralogous protein(s):''' [[YotE]]
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** ([[AccA]]-[[AccD]])-[[AccB]]-[[AccC]] (acetyl-CoA carboxylase complex)
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU24340&redirect=T BSU24340]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1BNC 1BNC] (the protein from ''E. coli'') {{PubMed\|7915138}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P49787 P49787]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24340]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/6.3.4.14 6.3.4.14], [http://www.expasy.org/enzyme/6.4.1.2 6.4.1.2]
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[accB]]-[[accC]]-[[yqhY]]'' {{PubMed\|7592499}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=accC_2530354_2531706_-1 accC] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 527 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 286 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 634 {{PubMed\|21395229}}
−
−	~~=Biological materials =~~
−	* '''Mutant:'''
−	** GP1478 (chromosomal ''accC''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [[SPINE]], available in [[Jörg Stülke]]'s lab'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
−
−	* '''FLAG-tag construct:'''
−	** GP1483 (''spc'', based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed> 15952903 12121720 15968460 </pubmed>~~
−	~~==Original Publications==~~
−	~~<pubmed>7592499, 7915138</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 128: / Line 128: @@
 =Biological materials =
 * '''Mutant:'''
 * '''Expression vector:'''
@@ Line 135: / Line 136: @@
 * '''GFP fusion:'''
-* '''two-hybrid system:'''
+* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
 * '''Antibody:'''

@@ Line 146: / Line 146: @@
 ==Reviews==
 <pubmed> 15952903 12121720 15968460 </pubmed>
 ==Original Publications==
-<pubmed> 7592499, 7915138, </pubmed>
+<pubmed>7592499, 7915138</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 145: / Line 145: @@
 =References=
 ==Reviews==
-<pubmed> 15952903 12121720 </pubmed>
+<pubmed> 15952903 12121720 15968460 </pubmed>
 ==Original Publications==
 <pubmed> 7592499, 7915138, </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 41: / Line 41: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
-<br/><br/><br/><br/>
+<br/><br/>
 = [[Categories]] containing this gene/protein =
@@ Line 72: / Line 68: @@
 === Additional information===
 =The protein=
@@ Line 122: / Line 115: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=accC_2530354_2531706_-1 accC] {{PubMed|22383849}}
-* '''Sigma factor:'''
+* '''[[Sigma factor]]:'''
 * '''Regulation:'''
@@ Line 152: / Line 145: @@
 =References=
 ==Reviews==
-<pubmed> 15952903 </pubmed>
+<pubmed> 15952903 12121720 </pubmed>
 ==Original Publications==
 <pubmed> 7592499, 7915138, </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 129: / Line 129: @@
 * '''Additional information:'''
-** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 286 {{PubMed|21395229}}
+=Biological materials =
 * '''Mutant:'''

@@ Line 131: / Line 131: @@
 ** number of protein molecules per cell (minimal medium with glucose and ammonium): 1798 {{PubMed|24696501}}
 ** number of protein molecules per cell (complex medium with amino acids, without glucose): 6207 {{PubMed|24696501}}
 =Biological materials =
 * '''Mutant:'''