AccB - Revision history

Dtoedter: /* Biological materials */

2015-04-02T11:46:19Z

‎Biological materials

Jgundlach: /* Biological materials */

2015-04-01T12:26:23Z

‎Biological materials

Jgundlach: /* Biological materials */

2015-04-01T12:25:25Z

‎Biological materials

Jgundlach: /* Biological materials */

2015-04-01T12:24:09Z

‎Biological materials

Dtoedter: /* Biological materials */

2015-03-06T10:23:51Z

‎Biological materials

Dtoedter: /* Biological materials */

2015-03-06T09:54:09Z

‎Biological materials

Jstuelk: /* Original Publications */

2014-10-10T16:42:54Z

‎Original Publications

Jstuelk: /* Original Publications */

2014-10-10T16:42:39Z

‎Original Publications

Jstuelk: /* Reviews */

2014-10-10T07:48:53Z

‎Reviews

Jstuelk: /* Reviews */

2014-06-05T16:39:09Z

‎Reviews

← Older revision		Revision as of 11:46, 2 April 2015
Line 1:		Line 1:
−	* '''Description:~~''' acetyl-CoA carboxylase (biotin carboxyl carrier subunit) <br/><br/>~~	+	{{#bank: AccB}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''accB''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''fabE, yqhW ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| acetyl-CoA carboxylase (biotin carboxyl carrier subunit)~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| production of malonyl-CoA, the substrate for fatty acid biosynthesis~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU24350 accB]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=AccB AccB]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=accB accB]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 17 kDa, 4.394~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 477 bp, 159 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[accC]]'', ''[[spoIIIAH]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU24350 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU24350 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU24350 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:accB_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=accB_2531718_2532197_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:accB_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU24350]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[biosynthesis of lipids]]}},~~
−	~~{{SubtiWiki category\|[[essential genes]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU24350
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU24350&redirect=T BSU24350]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/accBC-yqhY.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11383]
−
−	~~=== Additional information===~~
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−
−	* '''Protein family:'''
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:''' biotin
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** ([[AccA]]-[[AccD]])-[[AccB]]-[[AccC]] (acetyl-CoA carboxylase complex)
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU24350&redirect=T BSU24350]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1BDO 1BDO] (the protein from ''E. coli'') {{PubMed\|8747466}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P49786 P49786]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24350]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/6.4.1.2 6.4.1.2]
−
−	~~=== Additional information===~~
−
−	~~AccB binds to StrepTactin, and may be co-purified when purifying Strep-tagged proteins by [[SPINE]].~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[accB]]-[[accC]]-[[yqhY]]'' {{PubMed\|7592499}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=accB_2531718_2532197_-1 accB] {{PubMed\|22383849}}
−
−	* '''Sigma factor:'''
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 3355 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 889 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 28 {{PubMed\|21395229}}
−
−	~~=Biological materials =~~
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−	** GP1477 (chromosomal ''accB''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [[SPINE]], available in [[Jörg Stülke]]'s lab'''
−	** pGP2649: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]], available in [[Jörg Stülke]]'s lab
−	** pGP2650: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]], available in [[Jörg Stülke]]'s lab
−	** pGP2652: IPTG inducible expression, purification in ''E. coli'' with C-terminal Strep-tag, in [[pGP574]], available in [[Jörg Stülke]]'s lab
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
−
−	* '''FLAG-tag construct:'''
−	** GP1484 (''mls'', based on [[pGP1087]]), available in [[Jörg Stülke]]'s lab
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed> 15952903 12121720 15968460 </pubmed>~~
−
−	~~==Original Publications==~~
−	~~<pubmed>7592499, 8747466, </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 134: / Line 134: @@
 ** pGP2649: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]], available in [[Jörg Stülke]]'s lab
 ** pGP2650: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]], available in [[Jörg Stülke]]'s lab
 * '''lacZ fusion:'''

@@ Line 133: / Line 133: @@
 ** GP1477 (chromosomal ''accB''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [[SPINE]], available in [[Jörg Stülke]]'s lab'''
 ** pGP2649: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]], available in [[Jörg Stülke]]'s lab
 * '''lacZ fusion:'''

@@ Line 131: / Line 131: @@
 * '''Expression vector:'''
 ** GP1477 (chromosomal ''accB''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [[SPINE]], available in [[Jörg Stülke]]'s lab'''
 * '''lacZ fusion:'''

← Older revision		Revision as of 10:23, 6 March 2015
Line 139:		Line 139:
	* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab		* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab

		+	* '''FLAG-tag construct:'''
		+	** GP1484 (''mls'', based on [[pGP1087]]), available in [[Jörg Stülke]]'s lab

	* '''Antibody:'''		* '''Antibody:'''

@@ Line 131: / Line 131: @@
 * '''Expression vector:'''
 * '''lacZ fusion:'''
@@ Line 136: / Line 137: @@
 * '''GFP fusion:'''
-* '''two-hybrid system:'''
+* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
 * '''Antibody:'''

@@ Line 149: / Line 149: @@
 ==Original Publications==
-<pubmed>7592499, 8747466, 7592499 </pubmed>
+<pubmed>7592499, 8747466, </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 146: / Line 146: @@
 =References=
 ==Reviews==
-<pubmed> 15952903 12121720 </pubmed>
+<pubmed> 15952903 12121720 15968460 </pubmed>
 ==Original Publications==