AccA - Revision history

Dtoedter: /* Biological materials */

2015-04-02T11:47:40Z

‎Biological materials

Dtoedter: /* Biological materials */

2015-03-06T10:34:36Z

‎Biological materials

Jstuelk: /* Reviews */

2014-10-10T07:49:04Z

‎Reviews

Jstuelk: /* Reviews */

2014-06-05T16:38:45Z

‎Reviews

134.76.70.252 at 14:02, 17 April 2014

2014-04-17T14:02:25Z

Jstuelk at 14:01, 17 April 2014

2014-04-17T14:01:05Z

Jstuelk: /* Expression and regulation */

2014-04-17T13:59:29Z

‎Expression and regulation

Jstuelk: Reverted edits by 134.76.70.252 (talk) to last revision by Jstuelk

2014-04-17T13:57:22Z

Reverted edits by 134.76.70.252 (talk) to last revision by Jstuelk

134.76.70.252 at 13:52, 17 April 2014

2014-04-17T13:52:52Z

Jstuelk: Reverted edits by 134.76.70.252 (talk) to last revision by 134.76.38.147

2014-04-17T13:36:01Z

Reverted edits by 134.76.70.252 (talk) to last revision by 134.76.38.147

← Older revision		Revision as of 11:47, 2 April 2015
Line 1:		Line 1:
−	* '''Description:~~''' acetyl-CoA carboxylase (alpha subunit) <br/><br/>~~	+	{{#bank: AccA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''accA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| acetyl-CoA carboxylase (alpha subunit))~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| production of malonyl-CoA, the substrate for fatty acid biosynthesis~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29200 accA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=AccA AccA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=accA accA]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 36 kDa, 6.087~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 975 bp, 325 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[pfkA]]'', ''[[accD]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29200 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29200 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29200 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:accA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=accA_2987731_2988708_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:accA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29200]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[biosynthesis of lipids]]}},~~
−	~~{{SubtiWiki category\|[[essential genes]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[Spo0A regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU29200
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29200&redirect=T BSU29200]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/accDA.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12557]
−
−	~~=== Additional information===~~
−
−
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' ATP + acetyl-CoA + HCO<sub>3</sub><sup>-</sup> = ADP + phosphate + malonyl-CoA (according to Swiss-Prot)
−
−	* '''Protein family:''' accA family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** ([[AccA]]-[[AccD]])-[[AccB]]-[[AccC]] (acetyl-CoA carboxylase complex)
−
−	* '''[[Localization]]:''' cytoplasm (according to Swiss-Prot), Membrane-proximal (Spotty) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29200&redirect=T BSU29200]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=2F9I 2F9I] (from ''Staphylococcus aureus''; equivalent to [[AccD]]-[[AccA]], 64% identity) {{PubMed\|16460018}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/O34847 O34847]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29200]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/6.4.1.2 6.4.1.2]
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[accD]]-[[accA]]'' {{PubMed\|22383849}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=accA_2987731_2988708_-1 accA] {{PubMed\|22383849}}
−
−	* '''Sigma factor:'''
−
−	* '''Regulation:'''
−	** expressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
−
−	* '''Regulatory mechanism:'''
−	** [[Spo0A]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1047 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 795 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 910 {{PubMed\|21395229}}
−
−	~~=Biological materials =~~
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−	** purification from ''B. subtilis'' with an C-terminal Strep-tag, for [[SPINE]], (in [[pGP382]]): pGP1723, available in [[Jörg Stülke]]'s lab
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
−
−	* '''FLAG-tag construct:'''
−	** GP1487 (''spc'', based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed> 15952903, 17919287 12121720 15968460 </pubmed>~~
−
−	~~==Original Publications==~~
−	~~<pubmed>15066985, 16460018,14651647,16479537 12663926 22383849</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 133: / Line 133: @@
 * '''Expression vector:'''
 * '''lacZ fusion:'''
@@ Line 138: / Line 139: @@
 * '''GFP fusion:'''
-* '''two-hybrid system:'''
+* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
 * '''Antibody:'''

@@ Line 148: / Line 148: @@
 =References=
 ==Reviews==
-<pubmed> 15952903, 17919287 12121720 </pubmed>
+<pubmed> 15952903, 17919287 12121720 15968460 </pubmed>
 ==Original Publications==

@@ Line 148: / Line 148: @@
 =References=
 ==Reviews==
-<pubmed> 15952903, 17919287 </pubmed>
+<pubmed> 15952903, 17919287 12121720 </pubmed>
 ==Original Publications==

@@ Line 125: / Line 125: @@
 * '''Additional information:'''
 =Biological materials =
 * '''Mutant:'''

← Older revision		Revision as of 14:01, 17 April 2014
Line 125:		Line 125:

	* '''Additional information:'''		* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1047 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 795 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 910 {{PubMed\|21395229}}

	=Biological materials =		=Biological materials =

← Older revision		Revision as of 13:59, 17 April 2014
Line 125:		Line 125:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1047 {{PubMed\|21395229}}
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 795 {{PubMed\|21395229}}
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 910 {{PubMed\|21395229}}

	=Biological materials =		=Biological materials =