View source for Methods

Here you can find a list of methods for working with ''Bacillus subtilis'':

== Media ==
* [[C minimal medium]]
* [[M9 minimal medium]]

==Analysis of protein-protein interactions==
* Bacterial Two Hybrid: [[BACTH]]

* [[SPINE]]: A method to detect ''in vivo'' [[protein-protein interactions]]

* ''in vivo'' detection of [[protein-protein interactions]] using [[DivIVA]] and GFP {{PubMed|19698693}}

==Analysis of membrane proteins==
* application of a split green fluorescent protein reporter  {{PubMed|22285978}}

== Antibodies ==
* List of available [[Antibodies]]

== Protein expression ==
* the LIKE system {{PubMed|23110498}}

==Strain construction==
* introduction of markerfree deletions: {{PubMed|24140578,23916947,23911571,22422839,20057169}}
* generation of markerfree mutations: {{PubMed|20134232,21542786,24282588}}
* an improved method for transformation: {{PubMed|24838881}}

==RNA analysis==
* Mechanical cell disruption and extraction of RNA from ''B. subtilis'': [[Media:SOP-RNA.pdf]]

==Genetic work with non-standard strains==
* transformation of wild-type ''B. subtilis'' strains incl. NCIB3610: {{PubMed|20300532}}
* efficient electroporation of ''B. subtilis'' {{PubMed|22387342}}
* a novel transformation protocol for ''B. subtilis'' DB104 {{PubMed|22395911}}

==General procedures==
* Standard operation procedures ([[SOP]]s) of the SYSMO-BACELL consortium

==Microscopic techniques==
* Live cell imaging of ''B. subtilis'' cells using automated time-lapse microscopy {{PubMed|21841760}}
* Assay of gene expression dynamics using live cell imaging {{PubMed|22179594}}
* Visualization and quantification of gene expression heterogeneity in growing microbial cells {{PubMed|23874729}}
* GFP variants specifically optimizd for use in ''B. subtilis'' {{PubMed|23956387}}
* Quantitative analysis of cell types by comparing microscoy images {{PubMed|25448819}}

==Transposon mutagenesis==
* mariner transposon mutagenesis for random inducible-expression insertions and transcriptional reporter fusion insertions  {{PubMed|22113911}}

==Protein-DNA interactions==
* ChAP-chip: A modified ChIP-chip protocol for the ''in vivo'' identification of binding sites of DNA-binding proteins {{PubMed|17932079}}

==Genome engineering==
* generalized bacterial genome editing using mobile group II introns and Cre-lox {{PubMed|24002656}}
* genome engineering using a synthetic gene circuit {{PubMed|25552415}}
* A review on genome engineering:
<pubmed>23340847 </pubmed>

== Visual descriptions of methods for ''B. subtilis'' in the ''Journal of Visual Experimentation'' ==
<pubmed> 24473333 24457605 24300024 24300445 22371091 20142799 21841760 </pubmed>

==Key references==
<pubmed>22179594 20057169 20134232 21542786 20300532 19698693 17932079 21841760 17994626 23956387 22285978,22113911 24002656 24282588 </pubmed>