RibU
- Description: riboflavin ECF transporter, S protein
| Gene name | ribU |
| Synonyms | ypaA |
| Essential | no |
| Product | S protein of riboflavin ECF transporter |
| Function | riboflavin uptake |
| Gene expression levels in SubtiExpress: ribU | |
| Interactions involving this protein in SubtInteract: RibU | |
| Metabolic function and regulation of this protein in SubtiPathways: ribU | |
| MW, pI | 20 kDa, 10.12 |
| Gene length, protein length | 570 bp, 190 aa |
| Immediate neighbours | fer, ypzE |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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| Expression at a glance PubMed 500px | |
Contents
Categories containing this gene/protein
ECF transporter, biosynthesis of cofactors, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU23050
Phenotypes of a mutant
Database entries
- BsubCyc: BSU23050
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Localization: membrane
Database entries
- BsubCyc: BSU23050
- Structure: 3P5N (RibU from Staphylococcus aureus)
- UniProt: P50726
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism: FMN-box: riboswitch, controls access of the ribosome to the Shine-Dalgarno sequence PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Dirk J Slotboom
Structural and mechanistic insights into prokaryotic energy-coupling factor transporters.
Nat Rev Microbiol: 2014, 12(2);79-87
[PubMed:24362466]
[WorldCat.org]
[DOI]
(I p)
Guus B Erkens, Maria Majsnerowska, Josy ter Beek, Dirk Jan Slotboom
Energy coupling factor-type ABC transporters for vitamin uptake in prokaryotes.
Biochemistry: 2012, 51(22);4390-6
[PubMed:22574898]
[WorldCat.org]
[DOI]
(I p)
Thomas Eitinger, Dmitry A Rodionov, Mathias Grote, Erwin Schneider
Canonical and ECF-type ATP-binding cassette importers in prokaryotes: diversity in modular organization and cellular functions.
FEMS Microbiol Rev: 2011, 35(1);3-67
[PubMed:20497229]
[WorldCat.org]
[DOI]
(I p)
Original publications
S A Sklyarova, A S Mironov
[Bacillus subtilis ypaA gene regulation mechanism involves FMN-binding sensor RNA].
Genetika: 2014, 50(3);364-8
[PubMed:25438558]
[WorldCat.org]
(P p)
Jennifer Frommer, Robert Hieronymus, Tamil Selvi Arunachalam, Sabine Heeren, Maria Jenckel, Anne Strahl, Bettina Appel, Sabine Müller
Preparation of modified long-mer RNAs and analysis of FMN binding to the ypaA aptamer from B. subtilis.
RNA Biol: 2014, 11(5);609-23
[PubMed:24755604]
[WorldCat.org]
[DOI]
(I p)
Jianing Song, Changge Ji, John Z H Zhang
Unveiling the gating mechanism of ECF transporter RibU.
Sci Rep: 2013, 3;3566
[PubMed:24356467]
[WorldCat.org]
[DOI]
(I e)
Martin Lehnik-Habrink, Marc Schaffer, Ulrike Mäder, Christine Diethmaier, Christina Herzberg, Jörg Stülke
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y.
Mol Microbiol: 2011, 81(6);1459-73
[PubMed:21815947]
[WorldCat.org]
[DOI]
(I p)
Peng Zhang, Jiawei Wang, Yigong Shi
Structure and mechanism of the S component of a bacterial ECF transporter.
Nature: 2010, 468(7324);717-20
[PubMed:20972419]
[WorldCat.org]
[DOI]
(I p)
Christian Vogl, Simon Grill, Oliver Schilling, Jörg Stülke, Matthias Mack, Jürgen Stolz
Characterization of riboflavin (vitamin B2) transport proteins from Bacillus subtilis and Corynebacterium glutamicum.
J Bacteriol: 2007, 189(20);7367-75
[PubMed:17693491]
[WorldCat.org]
[DOI]
(P p)
Wade C Winkler, Smadar Cohen-Chalamish, Ronald R Breaker
An mRNA structure that controls gene expression by binding FMN.
Proc Natl Acad Sci U S A: 2002, 99(25);15908-13
[PubMed:12456892]
[WorldCat.org]
[DOI]
(P p)
