yloB

yloB
168

ATP-driven Ca2+ pump, transports calcium ions from the mother cell to the forespore

Locus
BSU_15650
Molecular weight
97.10 kDa
Isoelectric point
8.71
Protein length
890 aaSequenceBlast
Gene length
2673 bpSequenceBlast
Function
accumulation of calcium in the spore
Product
ATP-driven Ca2+ pump
Essential
no
E.C.
3.6.3.8
Synonyms
yloB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0474 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,637,965  1,640,637
Phenotypes of a mutant
forms unstable spores PubMed
delayed spore Germination PubMed
defective in Biofilm formation PubMed
The protein
Catalyzed reaction/ biological activity
transport of calcium ions from the mother cell to the forespore PubMed
there is no indication for an implication of YloB in Mg2+ uptake (based on mutant phenotypes, failure to complement mutants defective in Mg2+ uptake) PubMed
ATP + Ca2+ + H2O --> ADP + Ca2+ + H+ + phosphate (according to UniProt)
Protein family
cation transport ATPase (P-type) (TC 3.A.3) family (according to UniProt)
Structure
4YCM (PDB) (the calcium pump with bound marine macrolide BLS from O. cuniculus, 35% identity) PubMed
O34431 (AlphaFold)
Localization
cell membrane PubMed
Expression and Regulation
Operons
Genes
yloB-yloC-remA-gmk-yloH
Description
PubMed
Regulation
expressed during sporulation in the mother cell PubMed
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yloB->yloH

2025-03-15 07:25:31

Jstuelk

149

b15c1f28629d3e15db14719be44c291dd5f28c5f

86CE620BD4E14CC259270F542702B302B7837CCD

Biological materials
Mutant
MGNA-B139 (yloB::erm), available at the NBRP B. subtilis, Japan
BKE15650 (yloB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCCGCTCGTCCACTCCC,  downstream forward: _UP4_TAAATTCATATGATATAATC
BKK15650 (yloB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCCGCTCGTCCACTCCC,  downstream forward: _UP4_TAAATTCATATGATATAATC
References
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7821A09DADC6EAF589A882F05CC34BE900E06F2A

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Time of last update: 2025-04-01 02:56:09

Author of last update: Jstuelk