yitS

yitS
168

fatty acid binding protein

Locus
BSU_11110
Molecular weight
31.12 kDa
Isoelectric point
5.63
Protein length
283 aaSequenceBlast
Gene length
852 bpSequenceBlast
Function
phosphorylation of fatty acids
Product
fatty acid binding protein
Essential
no
Synonyms
yitS
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1307 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,187,700 → 1,188,551
The protein
Catalyzed reaction/ biological activity
binds and presents fatty acids for phosphorylation to FakA (based on findings for homologous proteins from Staphylococcus aureus) PubMed
Domains
DegV domain (aa 3-282) (according to UniProt)
Structure
1PZX (PDB) (Geobacillus stearothermophilus)
P70945 (AlphaFold)
Paralogous protein(s)
DegV
Expression and Regulation
Operons
Genes
yitS
Description
PubMed
Open in new tab

yitS

2025-03-23 12:28:50

Jstuelk

70

812504db80ea64f4355e7f50b0d765470f5e248f

9F51780C04B3A95B15B52D0F3CD52618F287D277

Biological materials
Mutant
GP3756 (ΔyitS::aphA3), available in Jörg Stülke's lab
MGNA-B196 (yitS::erm), available at the NBRP B. subtilis, Japan
BKE11110 (ΔyitS::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTGCTTCCCTTCTTTC,  downstream forward: _UP4_TGAGCAAACAAAAACAGTCA
BKK11110 (ΔyitS::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTGCTTCCCTTCTTTC,  downstream forward: _UP4_TGAGCAAACAAAAACAGTCA
Expression vectors
pGP3626 (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMOadapt), available in Jörg Stülke's lab
References
Research papers
Loading

C13F1870B014F8B2C16D9B169F40747DE5B51B85

Page visits: 2973

Time of last update: 2025-04-01 18:50:09

Author of last update: Dennis.wicke